Recovering DNA and Optimizing PCR Conditions from Microdissected Formalin-Fixed and Paraffin-Embedded Materials
Microdissection is a powerful technique in molecular pathology and genetic investigations. To detect genetic alterations such as gene mutation or deletion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level...
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| Veröffentlicht in: | Pathobiology (Basel) 2000-01, Vol.68 (4-5), p.215-217 |
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| creator | Ren, Zhi-Ping Sällström, Jan Sundström, Christer Nistér, Monica Olsson, Yngve |
| description | Microdissection is a powerful technique in molecular pathology and genetic investigations. To detect genetic alterations such as gene mutation or deletion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level of the abnormality. The major obstacle in clinical research is to obtain sufficient and qualified DNA from a small amount of formalin-fixed and paraffin-embedded materials. We have successfully modified our previous protocols and overcome the difficulties of recovery of DNA. After these modifications, almost every single formalin-fixed and paraffin-embedded specimen has been successfully amplified in the required DNA region. |
| doi_str_mv | 10.1159/000055926 |
| format | Article |
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To detect genetic alterations such as gene mutation or deletion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level of the abnormality. The major obstacle in clinical research is to obtain sufficient and qualified DNA from a small amount of formalin-fixed and paraffin-embedded materials. We have successfully modified our previous protocols and overcome the difficulties of recovery of DNA. 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To detect genetic alterations such as gene mutation or deletion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level of the abnormality. The major obstacle in clinical research is to obtain sufficient and qualified DNA from a small amount of formalin-fixed and paraffin-embedded materials. We have successfully modified our previous protocols and overcome the difficulties of recovery of DNA. After these modifications, almost every single formalin-fixed and paraffin-embedded specimen has been successfully amplified in the required DNA region.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>11279349</pmid><doi>10.1159/000055926</doi><tpages>3</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Karger Journals |
| subjects | Deoxyribonucleic acid Dissection - methods DNA DNA - isolation & purification Fixatives Formaldehyde Humans Paraffin Embedding - methods Polymerase Chain Reaction - methods Tissue Fixation - methods |
| title | Recovering DNA and Optimizing PCR Conditions from Microdissected Formalin-Fixed and Paraffin-Embedded Materials |
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