Sialic acids of young and old red blood cells in healthy subjects
A quite simple method for obtaining young and old erythrocytes is presented. Mean corpuscular volume, glutamic oxaloacetic transaminase activity which is one of the best age parameters, and contents of total sialic acids in erythrocyte membranes were determined in whole erythrocyte population and in...
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| Veröffentlicht in: | Medical science monitor 2000-09, Vol.6 (5), p.871-874 |
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| creator | Jakubowska-Solarska, B Solski, J |
| description | A quite simple method for obtaining young and old erythrocytes is presented. Mean corpuscular volume, glutamic oxaloacetic transaminase activity which is one of the best age parameters, and contents of total sialic acids in erythrocyte membranes were determined in whole erythrocyte population and in low (old) and high (young) density fractions separated by centrifugation of packed cells obtained from 23 healthy subjects. Glutamic oxaloacetic transaminase (GOT) activity, and contents of total sialic acids in erythrocyte membranes were significantly higher in young cells then in old ones, or in the whole population of erythrocytes. The presented results suggest that this method is simple for obtaining two different fractions of erythrocytes and that erythrocytes lose intact sialic acids together with their membrane during erythrocytes senescence. |
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Mean corpuscular volume, glutamic oxaloacetic transaminase activity which is one of the best age parameters, and contents of total sialic acids in erythrocyte membranes were determined in whole erythrocyte population and in low (old) and high (young) density fractions separated by centrifugation of packed cells obtained from 23 healthy subjects. Glutamic oxaloacetic transaminase (GOT) activity, and contents of total sialic acids in erythrocyte membranes were significantly higher in young cells then in old ones, or in the whole population of erythrocytes. 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Mean corpuscular volume, glutamic oxaloacetic transaminase activity which is one of the best age parameters, and contents of total sialic acids in erythrocyte membranes were determined in whole erythrocyte population and in low (old) and high (young) density fractions separated by centrifugation of packed cells obtained from 23 healthy subjects. Glutamic oxaloacetic transaminase (GOT) activity, and contents of total sialic acids in erythrocyte membranes were significantly higher in young cells then in old ones, or in the whole population of erythrocytes. The presented results suggest that this method is simple for obtaining two different fractions of erythrocytes and that erythrocytes lose intact sialic acids together with their membrane during erythrocytes senescence.</description><subject>Adult</subject><subject>Aspartate Aminotransferases - blood</subject><subject>Centrifugation, Density Gradient</subject><subject>Erythrocyte Aging - physiology</subject><subject>Erythrocyte Indices</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Erythrocytes - cytology</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Sialic Acids - blood</subject><issn>1234-1010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jz1PwzAYhD2AaCn8BeSJLZL9vk7sjlXFl1SJAZgjf4W6cuIQJ0P-PUG00-l0j053V2TNAUXBGWcrcpvziTFQFStvyIpzYEqAWJPdR9AxWKptcJmmhs5p6r6p7hxN0dHBO2piSo5aH2OmoaNHr-N4nGmezMnbMd-R60bH7O_PuiFfz0-f-9fi8P7ytt8dip4DjIWUiFtEK3RVltiAsMIZyZFjgxKNB6gqpszilghZszAOYKsUE45Z73BDHv97-yH9TD6PdRvy3yrd-TTlWkKpUCEs4MMZnEzrXd0PodXDXF9O4y-6j1AQ</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Jakubowska-Solarska, B</creator><creator>Solski, J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200009</creationdate><title>Sialic acids of young and old red blood cells in healthy subjects</title><author>Jakubowska-Solarska, B ; Solski, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-7733933c4a6553f24c4db71313f373be226608b3f324c30f53fd2298804d0ced3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Centrifugation, Density Gradient</topic><topic>Erythrocyte Aging - physiology</topic><topic>Erythrocyte Indices</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Erythrocytes - cytology</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Sialic Acids - blood</topic><toplevel>online_resources</toplevel><creatorcontrib>Jakubowska-Solarska, B</creatorcontrib><creatorcontrib>Solski, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Medical science monitor</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jakubowska-Solarska, B</au><au>Solski, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sialic acids of young and old red blood cells in healthy subjects</atitle><jtitle>Medical science monitor</jtitle><addtitle>Med Sci Monit</addtitle><date>2000-09</date><risdate>2000</risdate><volume>6</volume><issue>5</issue><spage>871</spage><epage>874</epage><pages>871-874</pages><issn>1234-1010</issn><abstract>A quite simple method for obtaining young and old erythrocytes is presented. Mean corpuscular volume, glutamic oxaloacetic transaminase activity which is one of the best age parameters, and contents of total sialic acids in erythrocyte membranes were determined in whole erythrocyte population and in low (old) and high (young) density fractions separated by centrifugation of packed cells obtained from 23 healthy subjects. Glutamic oxaloacetic transaminase (GOT) activity, and contents of total sialic acids in erythrocyte membranes were significantly higher in young cells then in old ones, or in the whole population of erythrocytes. The presented results suggest that this method is simple for obtaining two different fractions of erythrocytes and that erythrocytes lose intact sialic acids together with their membrane during erythrocytes senescence.</abstract><cop>United States</cop><pmid>11208424</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Medical science monitor, 2000-09, Vol.6 (5), p.871-874 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aspartate Aminotransferases - blood Centrifugation, Density Gradient Erythrocyte Aging - physiology Erythrocyte Indices Erythrocyte Membrane - metabolism Erythrocytes - cytology Erythrocytes - metabolism Female Humans Male Middle Aged Sialic Acids - blood |
| title | Sialic acids of young and old red blood cells in healthy subjects |
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