Cryopreservation of isolated hepatocytes: Intracellular ice formation under various chemical and physical conditions

Kinetics of intracellular ice formation (IIF) for isolated rat hepatocytes was studied using a cryomicroscopy system. The effect of the cooling rate on IIF was investigated between 20 and 400 °C/min in isotonic solution. At 50 °C/min and below, none of the hepatocytes underwent IIF; whereas at 150 °...

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Veröffentlicht in:	Cryobiology 1991-10, Vol.28 (5), p.436-444
Hauptverfasser:	Harris, Cheryl L., Toner, Mehmet, Hubel, Allison, Cravalho, Ernest G., Yarmush, Martin L., Tompkins, Ronald G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biological material (laboratory animals, preparation of biological tracers, etc.) Cryopreservation - methods Dimethyl Sulfoxide Female Fundamental and applied biological sciences. Psychology General aspects Ice In Vitro Techniques Kinetics Liver - cytology Rats Rats, Inbred Lew
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