Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis
Specific antigen of G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel...
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| Veröffentlicht in: | International journal for parasitology 1991-10, Vol.21 (6), p.677-687 |
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| container_title | International journal for parasitology |
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| creator | Nopparatana, Chamnong Setasuban, Prasert Chaicumpa, Wanpen Tapchaisri, Pramuan |
| description | Specific antigen of
G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and staining, Western blot analysis and isoelectric focussing. The specific antigen which has a pI of 8.5 was used as antigen in the indirect enzyme-linked immunosorbent assay (ELISA) to detect specific antibody in four groups of individuals, namely five parasitologically diagnosed gnathostomiasis patients (group 1); 15 clinically diagnosed gnathostomiasis patients (group 2); 136 patients with other parasitic infections (group 3); and 25 normal healthy parasite-free controls. Sensitivity, specificity and predictive values (positive and negative) of the assay were 100%. |
| doi_str_mv | 10.1016/0020-7519(91)90079-M |
| format | Article |
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G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and staining, Western blot analysis and isoelectric focussing. The specific antigen which has a pI of 8.5 was used as antigen in the indirect enzyme-linked immunosorbent assay (ELISA) to detect specific antibody in four groups of individuals, namely five parasitologically diagnosed gnathostomiasis patients (group 1); 15 clinically diagnosed gnathostomiasis patients (group 2); 136 patients with other parasitic infections (group 3); and 25 normal healthy parasite-free controls. 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G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and staining, Western blot analysis and isoelectric focussing. The specific antigen which has a pI of 8.5 was used as antigen in the indirect enzyme-linked immunosorbent assay (ELISA) to detect specific antibody in four groups of individuals, namely five parasitologically diagnosed gnathostomiasis patients (group 1); 15 clinically diagnosed gnathostomiasis patients (group 2); 136 patients with other parasitic infections (group 3); and 25 normal healthy parasite-free controls. Sensitivity, specificity and predictive values (positive and negative) of the assay were 100%.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1757195</pmid><doi>10.1016/0020-7519(91)90079-M</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Antigens, Helminth - isolation & purification Biological and medical sciences Diseases caused by nematodes Enzyme-Linked Immunosorbent Assay Gnathostoma - immunology Helminthic diseases Humans Infectious diseases Medical sciences Miscellaneous Nematode Infections - diagnosis Parasitic diseases Predictive Value of Tests Tropical medicine |
| title | Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis |
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