A novel calcitonin carboxyl-terminal peptide produced in medullary thyroid carcinoma by alternative RNA processing of the calcitonin/calcitonin gene-related peptide gene
The calcitonin/calcitonin gene-related peptide gene expresses two different mRNAs by tissue-specific alternative processing. The calcitonin mRNA is produced in thyroid C cells by splicing of the first three exons to the fourth polyadenylated exon. It encodes a protein precursor containing an amino-t...
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| Veröffentlicht in: | The Journal of biological chemistry 1991-12, Vol.266 (36), p.24627-24631 |
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| creator | MINVIELLE, S GISCARD-DARTEVELLE, S COHEN, R TABOULET, J LABYE, F JULIENNE, A RIVAILLE, P MILHAUD, G MOUKHTAR, M. S.K LASMOLES, F |
| description | The calcitonin/calcitonin gene-related peptide gene expresses two different mRNAs by tissue-specific alternative processing.
The calcitonin mRNA is produced in thyroid C cells by splicing of the first three exons to the fourth polyadenylated exon.
It encodes a protein precursor containing an amino-terminal peptide, calcitonin, and a carboxyl-terminal peptide (CCP I).
Calcitonin gene-related peptide (CGRP) mRNA is produced in neuronal cells by splicing of the three common exons to the fifth
exon and the polyadenylated sixth exon, leading to the production of a CGRP precursor. Our studies concerning the expression
of the calcitonin/CGRP gene in human medullary thyroid carcinoma revealed the presence of a new RNA transcript. Amplification
by polymerase chain reaction and direct sequencing showed that the novel transcript is composed of exons 1, 2, and 3, part
of exon 4, exon 5, and a polyadenylated exon 6. This transcript contains an open reading frame coding for the known amino-terminal
and calcitonin peptides, as well as for a novel calcitonin carboxyl-terminal peptide, CCP II. This third alternative pathway
utilizes an internal donor site within the exon coding for calcitonin. The presence of CCP II was demonstrated in plasma and
thyroidal tissues of medullary thyroid carcinoma patients, implying that this novel mRNA is actively translated in medullary
thyroid carcinoma. |
| doi_str_mv | 10.1016/S0021-9258(18)54275-7 |
| format | Article |
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The calcitonin mRNA is produced in thyroid C cells by splicing of the first three exons to the fourth polyadenylated exon.
It encodes a protein precursor containing an amino-terminal peptide, calcitonin, and a carboxyl-terminal peptide (CCP I).
Calcitonin gene-related peptide (CGRP) mRNA is produced in neuronal cells by splicing of the three common exons to the fifth
exon and the polyadenylated sixth exon, leading to the production of a CGRP precursor. Our studies concerning the expression
of the calcitonin/CGRP gene in human medullary thyroid carcinoma revealed the presence of a new RNA transcript. Amplification
by polymerase chain reaction and direct sequencing showed that the novel transcript is composed of exons 1, 2, and 3, part
of exon 4, exon 5, and a polyadenylated exon 6. This transcript contains an open reading frame coding for the known amino-terminal
and calcitonin peptides, as well as for a novel calcitonin carboxyl-terminal peptide, CCP II. This third alternative pathway
utilizes an internal donor site within the exon coding for calcitonin. The presence of CCP II was demonstrated in plasma and
thyroidal tissues of medullary thyroid carcinoma patients, implying that this novel mRNA is actively translated in medullary
thyroid carcinoma.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)54275-7</identifier><identifier>PMID: 1761559</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Calcitonin - biosynthesis ; Calcitonin - blood ; Calcitonin - metabolism ; calcitonin gene-related peptide ; Calcitonin Gene-Related Peptide - genetics ; Chromatography, Affinity ; DNA, Neoplasm ; Exons ; Fundamental and applied biological sciences. Psychology ; genes ; Humans ; Immunohistochemistry ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Peptide Fragments - biosynthesis ; Peptide Fragments - blood ; Peptide Fragments - metabolism ; Polymerase Chain Reaction ; Protein Biosynthesis ; RNA ; RNA Splicing ; RNA, Messenger - metabolism ; RNA, Neoplasm - metabolism ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - metabolism ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>The Journal of biological chemistry, 1991-12, Vol.266 (36), p.24627-24631</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-b131b2ae3ca8a90398e599dbd62ea1d09717fcf8fd03363882af62f1751c52433</citedby><cites>FETCH-LOGICAL-c440t-b131b2ae3ca8a90398e599dbd62ea1d09717fcf8fd03363882af62f1751c52433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5149761$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1761559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MINVIELLE, S</creatorcontrib><creatorcontrib>GISCARD-DARTEVELLE, S</creatorcontrib><creatorcontrib>COHEN, R</creatorcontrib><creatorcontrib>TABOULET, J</creatorcontrib><creatorcontrib>LABYE, F</creatorcontrib><creatorcontrib>JULIENNE, A</creatorcontrib><creatorcontrib>RIVAILLE, P</creatorcontrib><creatorcontrib>MILHAUD, G</creatorcontrib><creatorcontrib>MOUKHTAR, M. S.K</creatorcontrib><creatorcontrib>LASMOLES, F</creatorcontrib><title>A novel calcitonin carboxyl-terminal peptide produced in medullary thyroid carcinoma by alternative RNA processing of the calcitonin/calcitonin gene-related peptide gene</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The calcitonin/calcitonin gene-related peptide gene expresses two different mRNAs by tissue-specific alternative processing.
The calcitonin mRNA is produced in thyroid C cells by splicing of the first three exons to the fourth polyadenylated exon.
It encodes a protein precursor containing an amino-terminal peptide, calcitonin, and a carboxyl-terminal peptide (CCP I).
Calcitonin gene-related peptide (CGRP) mRNA is produced in neuronal cells by splicing of the three common exons to the fifth
exon and the polyadenylated sixth exon, leading to the production of a CGRP precursor. Our studies concerning the expression
of the calcitonin/CGRP gene in human medullary thyroid carcinoma revealed the presence of a new RNA transcript. Amplification
by polymerase chain reaction and direct sequencing showed that the novel transcript is composed of exons 1, 2, and 3, part
of exon 4, exon 5, and a polyadenylated exon 6. This transcript contains an open reading frame coding for the known amino-terminal
and calcitonin peptides, as well as for a novel calcitonin carboxyl-terminal peptide, CCP II. This third alternative pathway
utilizes an internal donor site within the exon coding for calcitonin. The presence of CCP II was demonstrated in plasma and
thyroidal tissues of medullary thyroid carcinoma patients, implying that this novel mRNA is actively translated in medullary
thyroid carcinoma.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Calcitonin - biosynthesis</subject><subject>Calcitonin - blood</subject><subject>Calcitonin - metabolism</subject><subject>calcitonin gene-related peptide</subject><subject>Calcitonin Gene-Related Peptide - genetics</subject><subject>Chromatography, Affinity</subject><subject>DNA, Neoplasm</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Peptide Fragments - biosynthesis</subject><subject>Peptide Fragments - blood</subject><subject>Peptide Fragments - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Biosynthesis</subject><subject>RNA</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Neoplasm - metabolism</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EKkPhESp5gRAsQn2Jb8tRRQGpAomLxM5ynJMZo8QZ7KQwj9S3xOkMbXd4Y8vn-89vnx-hM0reUkLl-VdCGK0ME_o11W9EzZSo1CO0okTzigv64zFa3SFP0bOcf5KyakNP0AlVkgphVuhmjeN4DT32rvdhGmOI5Zia8c--ryZIQ4iuxzvYTaEFvEtjO3tocaEGaOe-d2mPp-0-jaFddD7EcXC42WPXF3V0U7gG_OXTepF6yDnEDR67IoEHjucPzDcQoUrQu6nY_PNdLp-jJ53rM7w47qfo--W7bxcfqqvP7z9erK8qX9dkqhrKacMccO-0M4QbDcKYtmklA0dbYhRVne901xLOJdeauU6yjipBvWA156fo1aFvefGvGfJkh5A9lK9GGOdsFRPKGMb-C1JJNFFSF1AcQJ_GnBN0dpfCUCZnKbFLlvY2S7sEZam2t1laVXRnR4O5KdO-Vx3CK_WXx7rLZYJdctGHfIcJWptC3mPbsNn-DglsE0a_hcEyKS2XltWSKf4XDbO2lw</recordid><startdate>19911225</startdate><enddate>19911225</enddate><creator>MINVIELLE, S</creator><creator>GISCARD-DARTEVELLE, S</creator><creator>COHEN, R</creator><creator>TABOULET, J</creator><creator>LABYE, F</creator><creator>JULIENNE, A</creator><creator>RIVAILLE, P</creator><creator>MILHAUD, G</creator><creator>MOUKHTAR, M. S.K</creator><creator>LASMOLES, F</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19911225</creationdate><title>A novel calcitonin carboxyl-terminal peptide produced in medullary thyroid carcinoma by alternative RNA processing of the calcitonin/calcitonin gene-related peptide gene</title><author>MINVIELLE, S ; GISCARD-DARTEVELLE, S ; COHEN, R ; TABOULET, J ; LABYE, F ; JULIENNE, A ; RIVAILLE, P ; MILHAUD, G ; MOUKHTAR, M. S.K ; LASMOLES, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-b131b2ae3ca8a90398e599dbd62ea1d09717fcf8fd03363882af62f1751c52433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Calcitonin - biosynthesis</topic><topic>Calcitonin - blood</topic><topic>Calcitonin - metabolism</topic><topic>calcitonin gene-related peptide</topic><topic>Calcitonin Gene-Related Peptide - genetics</topic><topic>Chromatography, Affinity</topic><topic>DNA, Neoplasm</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Peptide Fragments - biosynthesis</topic><topic>Peptide Fragments - blood</topic><topic>Peptide Fragments - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Biosynthesis</topic><topic>RNA</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Neoplasm - metabolism</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MINVIELLE, S</creatorcontrib><creatorcontrib>GISCARD-DARTEVELLE, S</creatorcontrib><creatorcontrib>COHEN, R</creatorcontrib><creatorcontrib>TABOULET, J</creatorcontrib><creatorcontrib>LABYE, F</creatorcontrib><creatorcontrib>JULIENNE, A</creatorcontrib><creatorcontrib>RIVAILLE, P</creatorcontrib><creatorcontrib>MILHAUD, G</creatorcontrib><creatorcontrib>MOUKHTAR, M. S.K</creatorcontrib><creatorcontrib>LASMOLES, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MINVIELLE, S</au><au>GISCARD-DARTEVELLE, S</au><au>COHEN, R</au><au>TABOULET, J</au><au>LABYE, F</au><au>JULIENNE, A</au><au>RIVAILLE, P</au><au>MILHAUD, G</au><au>MOUKHTAR, M. S.K</au><au>LASMOLES, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel calcitonin carboxyl-terminal peptide produced in medullary thyroid carcinoma by alternative RNA processing of the calcitonin/calcitonin gene-related peptide gene</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-12-25</date><risdate>1991</risdate><volume>266</volume><issue>36</issue><spage>24627</spage><epage>24631</epage><pages>24627-24631</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The calcitonin/calcitonin gene-related peptide gene expresses two different mRNAs by tissue-specific alternative processing.
The calcitonin mRNA is produced in thyroid C cells by splicing of the first three exons to the fourth polyadenylated exon.
It encodes a protein precursor containing an amino-terminal peptide, calcitonin, and a carboxyl-terminal peptide (CCP I).
Calcitonin gene-related peptide (CGRP) mRNA is produced in neuronal cells by splicing of the three common exons to the fifth
exon and the polyadenylated sixth exon, leading to the production of a CGRP precursor. Our studies concerning the expression
of the calcitonin/CGRP gene in human medullary thyroid carcinoma revealed the presence of a new RNA transcript. Amplification
by polymerase chain reaction and direct sequencing showed that the novel transcript is composed of exons 1, 2, and 3, part
of exon 4, exon 5, and a polyadenylated exon 6. This transcript contains an open reading frame coding for the known amino-terminal
and calcitonin peptides, as well as for a novel calcitonin carboxyl-terminal peptide, CCP II. This third alternative pathway
utilizes an internal donor site within the exon coding for calcitonin. The presence of CCP II was demonstrated in plasma and
thyroidal tissues of medullary thyroid carcinoma patients, implying that this novel mRNA is actively translated in medullary
thyroid carcinoma.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1761559</pmid><doi>10.1016/S0021-9258(18)54275-7</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | The Journal of biological chemistry, 1991-12, Vol.266 (36), p.24627-24631 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72579922 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Blotting, Northern Calcitonin - biosynthesis Calcitonin - blood Calcitonin - metabolism calcitonin gene-related peptide Calcitonin Gene-Related Peptide - genetics Chromatography, Affinity DNA, Neoplasm Exons Fundamental and applied biological sciences. Psychology genes Humans Immunohistochemistry Molecular and cellular biology Molecular genetics Molecular Sequence Data Peptide Fragments - biosynthesis Peptide Fragments - blood Peptide Fragments - metabolism Polymerase Chain Reaction Protein Biosynthesis RNA RNA Splicing RNA, Messenger - metabolism RNA, Neoplasm - metabolism Thyroid Neoplasms - genetics Thyroid Neoplasms - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | A novel calcitonin carboxyl-terminal peptide produced in medullary thyroid carcinoma by alternative RNA processing of the calcitonin/calcitonin gene-related peptide gene |
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