Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes
The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA–EF) and the lethal toxin (PA–LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor‐mediated endocytosis and passage through acidic vesicles. We inv...
Gespeichert in:
| Veröffentlicht in: | Cellular microbiology 2000-06, Vol.2 (3), p.259-264 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 264 |
|---|---|
| container_issue | 3 |
| container_start_page | 259 |
| container_title | Cellular microbiology |
| container_volume | 2 |
| creator | Guidi‐Rontani, Chantal Weber‐Levy, Martine Mock, Michèle Cabiaux, Véronique |
| description | The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA–EF) and the lethal toxin (PA–LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor‐mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta‐toxin, we observed free full‐size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles. |
| doi_str_mv | 10.1046/j.1462-5822.2000.00057.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72575433</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72575433</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4137-f2d598af2a1b3aec1b8425ff956bde4ebd1336fdc217dcaef5fb04eec94671c43</originalsourceid><addsrcrecordid>eNqNkE1PwyAYx4nRuDn9CoaTt1ag0HbxpIsvS2a8zHgkFB6yLrTM0sXt28teMq8eCG-_58_DDyFMSUoJz--XKeU5S0TJWMoIIWkcokg3Z2h4ujg_rSkfoKsQloTQvKD0Eg0oZaSI0BB9zTvVBue16mvfYm_xk9K1c-uAVdsvurgJ2EG_UC4eGOzBQKOwVbr3XWR050PA0BoffAO4gaaKgRCu0YVVLsDNcR6hz5fn-eQtmX28TiePs0RzmhWJZUaMS2WZolWmQNOq5ExYOxZ5ZYBDZWiW5dZoRgujFVhhK8IB9JjHr2iejdDdIXfV-e81hF42ddDgXGzCr4MsmCgEz7IIlgdw33EHVq66ulHdVlIid1LlUu58yZ07uZMq91LlJpbeHt9YVw2Yv8KjxQg8HICf2sH238Fy8j4VRfYLieyHOg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72575433</pqid></control><display><type>article</type><title>Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Guidi‐Rontani, Chantal ; Weber‐Levy, Martine ; Mock, Michèle ; Cabiaux, Véronique</creator><creatorcontrib>Guidi‐Rontani, Chantal ; Weber‐Levy, Martine ; Mock, Michèle ; Cabiaux, Véronique</creatorcontrib><description>The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA–EF) and the lethal toxin (PA–LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor‐mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta‐toxin, we observed free full‐size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles.</description><identifier>ISSN: 1462-5814</identifier><identifier>EISSN: 1462-5822</identifier><identifier>DOI: 10.1046/j.1462-5822.2000.00057.x</identifier><identifier>PMID: 11207582</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Antigens, Bacterial ; Bacillus anthracis - metabolism ; Bacterial Toxins - metabolism ; Carrier Proteins - physiology ; Cell Line ; Cell Membrane - physiology ; Cytosol - metabolism ; Endosomes - physiology ; Macrophages - microbiology ; Mice</subject><ispartof>Cellular microbiology, 2000-06, Vol.2 (3), p.259-264</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4137-f2d598af2a1b3aec1b8425ff956bde4ebd1336fdc217dcaef5fb04eec94671c43</citedby><cites>FETCH-LOGICAL-c4137-f2d598af2a1b3aec1b8425ff956bde4ebd1336fdc217dcaef5fb04eec94671c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1462-5822.2000.00057.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1462-5822.2000.00057.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11207582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guidi‐Rontani, Chantal</creatorcontrib><creatorcontrib>Weber‐Levy, Martine</creatorcontrib><creatorcontrib>Mock, Michèle</creatorcontrib><creatorcontrib>Cabiaux, Véronique</creatorcontrib><title>Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes</title><title>Cellular microbiology</title><addtitle>Cell Microbiol</addtitle><description>The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA–EF) and the lethal toxin (PA–LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor‐mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta‐toxin, we observed free full‐size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles.</description><subject>Animals</subject><subject>Antigens, Bacterial</subject><subject>Bacillus anthracis - metabolism</subject><subject>Bacterial Toxins - metabolism</subject><subject>Carrier Proteins - physiology</subject><subject>Cell Line</subject><subject>Cell Membrane - physiology</subject><subject>Cytosol - metabolism</subject><subject>Endosomes - physiology</subject><subject>Macrophages - microbiology</subject><subject>Mice</subject><issn>1462-5814</issn><issn>1462-5822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PwyAYx4nRuDn9CoaTt1ag0HbxpIsvS2a8zHgkFB6yLrTM0sXt28teMq8eCG-_58_DDyFMSUoJz--XKeU5S0TJWMoIIWkcokg3Z2h4ujg_rSkfoKsQloTQvKD0Eg0oZaSI0BB9zTvVBue16mvfYm_xk9K1c-uAVdsvurgJ2EG_UC4eGOzBQKOwVbr3XWR050PA0BoffAO4gaaKgRCu0YVVLsDNcR6hz5fn-eQtmX28TiePs0RzmhWJZUaMS2WZolWmQNOq5ExYOxZ5ZYBDZWiW5dZoRgujFVhhK8IB9JjHr2iejdDdIXfV-e81hF42ddDgXGzCr4MsmCgEz7IIlgdw33EHVq66ulHdVlIid1LlUu58yZ07uZMq91LlJpbeHt9YVw2Yv8KjxQg8HICf2sH238Fy8j4VRfYLieyHOg</recordid><startdate>200006</startdate><enddate>200006</enddate><creator>Guidi‐Rontani, Chantal</creator><creator>Weber‐Levy, Martine</creator><creator>Mock, Michèle</creator><creator>Cabiaux, Véronique</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200006</creationdate><title>Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes</title><author>Guidi‐Rontani, Chantal ; Weber‐Levy, Martine ; Mock, Michèle ; Cabiaux, Véronique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4137-f2d598af2a1b3aec1b8425ff956bde4ebd1336fdc217dcaef5fb04eec94671c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antigens, Bacterial</topic><topic>Bacillus anthracis - metabolism</topic><topic>Bacterial Toxins - metabolism</topic><topic>Carrier Proteins - physiology</topic><topic>Cell Line</topic><topic>Cell Membrane - physiology</topic><topic>Cytosol - metabolism</topic><topic>Endosomes - physiology</topic><topic>Macrophages - microbiology</topic><topic>Mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guidi‐Rontani, Chantal</creatorcontrib><creatorcontrib>Weber‐Levy, Martine</creatorcontrib><creatorcontrib>Mock, Michèle</creatorcontrib><creatorcontrib>Cabiaux, Véronique</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guidi‐Rontani, Chantal</au><au>Weber‐Levy, Martine</au><au>Mock, Michèle</au><au>Cabiaux, Véronique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes</atitle><jtitle>Cellular microbiology</jtitle><addtitle>Cell Microbiol</addtitle><date>2000-06</date><risdate>2000</risdate><volume>2</volume><issue>3</issue><spage>259</spage><epage>264</epage><pages>259-264</pages><issn>1462-5814</issn><eissn>1462-5822</eissn><abstract>The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA–EF) and the lethal toxin (PA–LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor‐mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta‐toxin, we observed free full‐size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11207582</pmid><doi>10.1046/j.1462-5822.2000.00057.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1462-5814 |
| ispartof | Cellular microbiology, 2000-06, Vol.2 (3), p.259-264 |
| issn | 1462-5814 1462-5822 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72575433 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antigens, Bacterial Bacillus anthracis - metabolism Bacterial Toxins - metabolism Carrier Proteins - physiology Cell Line Cell Membrane - physiology Cytosol - metabolism Endosomes - physiology Macrophages - microbiology Mice |
| title | Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T14%3A40%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Translocation%20of%20Bacillus%20anthracis%20lethal%20and%20oedema%20factors%20across%20endosome%20membranes&rft.jtitle=Cellular%20microbiology&rft.au=Guidi%E2%80%90Rontani,%20Chantal&rft.date=2000-06&rft.volume=2&rft.issue=3&rft.spage=259&rft.epage=264&rft.pages=259-264&rft.issn=1462-5814&rft.eissn=1462-5822&rft_id=info:doi/10.1046/j.1462-5822.2000.00057.x&rft_dat=%3Cproquest_cross%3E72575433%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72575433&rft_id=info:pmid/11207582&rfr_iscdi=true |