Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats
In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), and activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when ra...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1991-12, Vol.181 (3), p.1028-1034 |
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| creator | Morotomi, Masami LoGerfo, Paul Weinstein, I.Bernard |
| description | In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), and activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when rats were administered [
14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [
14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function. |
| doi_str_mv | 10.1016/0006-291X(91)92040-Q |
| format | Article |
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14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [
14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(91)92040-Q</identifier><identifier>PMID: 1764053</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Biological Transport ; Carbon Radioisotopes ; Colon - metabolism ; diacylglycerol ; Digestive System - metabolism ; Diglycerides - analysis ; Diglycerides - metabolism ; Fatty acids ; Feces - chemistry ; Food Analysis ; Fundamental and applied biological sciences. Psychology ; Intestinal Absorption ; Intestinal Mucosa - metabolism ; Kinetics ; Lipids ; Male ; mucosa ; Other biological molecules ; Rats ; Rats, Inbred F344 ; Time Factors</subject><ispartof>Biochemical and biophysical research communications, 1991-12, Vol.181 (3), p.1028-1034</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-e57daa1deb09c13fd5b4e4ecca7728db9e49f68b402863df3ba4781e6352a53a3</citedby><cites>FETCH-LOGICAL-c529t-e57daa1deb09c13fd5b4e4ecca7728db9e49f68b402863df3ba4781e6352a53a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X9192040Q$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5106214$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1764053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morotomi, Masami</creatorcontrib><creatorcontrib>LoGerfo, Paul</creatorcontrib><creatorcontrib>Weinstein, I.Bernard</creatorcontrib><title>Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), and activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when rats were administered [
14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [
14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Carbon Radioisotopes</subject><subject>Colon - metabolism</subject><subject>diacylglycerol</subject><subject>Digestive System - metabolism</subject><subject>Diglycerides - analysis</subject><subject>Diglycerides - metabolism</subject><subject>Fatty acids</subject><subject>Feces - chemistry</subject><subject>Food Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intestinal Absorption</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Kinetics</subject><subject>Lipids</subject><subject>Male</subject><subject>mucosa</subject><subject>Other biological molecules</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo67j6DxRyEFGwNZWvnlwEWVwVFnRBwVuoTqol2t0Zk25x_r09zrDelDrUoZ73pXgYewjiBQiwL4UQtpEOvjx18MxJoUVzfYttQDjRSBD6NtvcIHfZvVq_CQGgrTtjZ9BaLYzasI-XFHDg9CsUmlOenvNlN-N34jhFPtKMXR5SHXm35yEPeeLjEnJFnnseE4b98HXYByp54GniBed6n93pcaj04LTP2efLN58u3jVXH96-v3h91QQj3dyQaSMiROqEC6D6aDpNmkLAtpXb2DnSrrfbTgu5tSr2qkPdboGsMhKNQnXOnhx7dyX_WKjOfkw10DDgRHmpvpWmNUq1_wXBrqO0W0F9BEPJtRbq_a6kEcveg_AH4_6g0x90egf-j3F_vcYenfqXbqT4N3RUvN4fn-5YV9V9wSmkeoMZEFaCXrFXR4xWaT8TFV9DoilQTIXC7GNO__7jNxUDnSI</recordid><startdate>19911231</startdate><enddate>19911231</enddate><creator>Morotomi, Masami</creator><creator>LoGerfo, Paul</creator><creator>Weinstein, I.Bernard</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911231</creationdate><title>Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats</title><author>Morotomi, Masami ; LoGerfo, Paul ; Weinstein, I.Bernard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-e57daa1deb09c13fd5b4e4ecca7728db9e49f68b402863df3ba4781e6352a53a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Carbon Radioisotopes</topic><topic>Colon - metabolism</topic><topic>diacylglycerol</topic><topic>Digestive System - metabolism</topic><topic>Diglycerides - analysis</topic><topic>Diglycerides - metabolism</topic><topic>Fatty acids</topic><topic>Feces - chemistry</topic><topic>Food Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intestinal Absorption</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Kinetics</topic><topic>Lipids</topic><topic>Male</topic><topic>mucosa</topic><topic>Other biological molecules</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morotomi, Masami</creatorcontrib><creatorcontrib>LoGerfo, Paul</creatorcontrib><creatorcontrib>Weinstein, I.Bernard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morotomi, Masami</au><au>LoGerfo, Paul</au><au>Weinstein, I.Bernard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-12-31</date><risdate>1991</risdate><volume>181</volume><issue>3</issue><spage>1028</spage><epage>1034</epage><pages>1028-1034</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), and activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when rats were administered [
14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [
14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1764053</pmid><doi>10.1016/0006-291X(91)92040-Q</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1991-12, Vol.181 (3), p.1028-1034 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biological Transport Carbon Radioisotopes Colon - metabolism diacylglycerol Digestive System - metabolism Diglycerides - analysis Diglycerides - metabolism Fatty acids Feces - chemistry Food Analysis Fundamental and applied biological sciences. Psychology Intestinal Absorption Intestinal Mucosa - metabolism Kinetics Lipids Male mucosa Other biological molecules Rats Rats, Inbred F344 Time Factors |
| title | Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats |
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