A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue
A quantitative colorimetric assay using the oxidation–reduction indicator Alamar Blue® was developed to measure cytotoxicity of compounds against the protozoan parasite Leishmania. Absorbance increased linearly with the plating density of promastigotes of L. major MRHO/IR/76 vaccine strain up to at...
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| Veröffentlicht in: | Parasitology international 2000, Vol.48 (3), p.265-269 |
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| container_title | Parasitology international |
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| creator | Mikus, Judith Steverding, Dietmar |
| description | A quantitative colorimetric assay using the oxidation–reduction indicator Alamar Blue® was developed to measure cytotoxicity of compounds against the protozoan parasite
Leishmania. Absorbance increased linearly with the plating density of promastigotes of
L. major MRHO/IR/76 vaccine strain up to at least 2.5×10
6 cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue®. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue® assay permits a simple, reproducible and reliable method for screening antileishmanial drugs. |
| doi_str_mv | 10.1016/S1383-5769(99)00020-3 |
| format | Article |
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Leishmania. Absorbance increased linearly with the plating density of promastigotes of
L. major MRHO/IR/76 vaccine strain up to at least 2.5×10
6 cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue®. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue® assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.</description><identifier>ISSN: 1383-5769</identifier><identifier>EISSN: 1873-0329</identifier><identifier>DOI: 10.1016/S1383-5769(99)00020-3</identifier><identifier>PMID: 11227767</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Alamar Blue ; Amphotericin B - pharmacology ; Animals ; Anti-Bacterial Agents - pharmacology ; Antimony Sodium Gluconate - pharmacology ; Antiprotozoal Agents - pharmacology ; Colorimetry ; Colorimetry - methods ; Coloring Agents - chemistry ; Drug sensitivity ; In vitro assay ; Leishmania major ; Leishmania major - chemistry ; Leishmania major - drug effects ; Leishmaniasis, Cutaneous - drug therapy ; Leishmaniasis, Cutaneous - parasitology ; Oxazines ; Oxidation-Reduction ; Parasitic Sensitivity Tests - methods ; Paromomycin - pharmacology ; Reproducibility of Results ; Xanthenes</subject><ispartof>Parasitology international, 2000, Vol.48 (3), p.265-269</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-8131d2a40304a8701b5fa7ae679871cb6b1c10520e90084114e0c5e9f39e563d3</citedby><cites>FETCH-LOGICAL-c419t-8131d2a40304a8701b5fa7ae679871cb6b1c10520e90084114e0c5e9f39e563d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1383576999000203$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11227767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mikus, Judith</creatorcontrib><creatorcontrib>Steverding, Dietmar</creatorcontrib><title>A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue</title><title>Parasitology international</title><addtitle>Parasitol Int</addtitle><description>A quantitative colorimetric assay using the oxidation–reduction indicator Alamar Blue® was developed to measure cytotoxicity of compounds against the protozoan parasite
Leishmania. Absorbance increased linearly with the plating density of promastigotes of
L. major MRHO/IR/76 vaccine strain up to at least 2.5×10
6 cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue®. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue® assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.</description><subject>Alamar Blue</subject><subject>Amphotericin B - pharmacology</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimony Sodium Gluconate - pharmacology</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Colorimetry</subject><subject>Colorimetry - methods</subject><subject>Coloring Agents - chemistry</subject><subject>Drug sensitivity</subject><subject>In vitro assay</subject><subject>Leishmania major</subject><subject>Leishmania major - chemistry</subject><subject>Leishmania major - drug effects</subject><subject>Leishmaniasis, Cutaneous - drug therapy</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Oxazines</subject><subject>Oxidation-Reduction</subject><subject>Parasitic Sensitivity Tests - methods</subject><subject>Paromomycin - pharmacology</subject><subject>Reproducibility of Results</subject><subject>Xanthenes</subject><issn>1383-5769</issn><issn>1873-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu2zAQRYmiQZy4-YQWXBXJQsmMKIniqnCCvAADWbRdEzQ1tllIoktSQfz3kR9Bl13NLM7MxT2MfUW4RsDq5ieKWmSlrNSlUlcAkEMmPrEzrKXIQOTq87h_IBN2HuMfACylxFM2QcxzKSt5xmjGo-s2LXHrWx9cRyk4y8ex9g1PnkcbiHrehGHF7Tb55N-cdWnLzcq4PiY-JxfXnemd4UN0_YqnNfFmS3zWms4EftsO9IWdLE0b6eI4p-z3w_2vu6ds_vL4fDebZ7ZAlbIaBTa5KUBAYWoJuCiXRhqqpKol2kW1QItQ5kAKoC4QCwJbkloKRWUlGjFl3w9_N8H_HSgm3bloqW1NT36IWuZlVUulRrA8gDb4GAMt9WbsbsJWI-idX733q3fytFJ671eL8e7bMWBYdNT8uzoKHYEfB4DGmq-Ogo7WUW-pcYFs0o13_4l4ByXYinQ</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Mikus, Judith</creator><creator>Steverding, Dietmar</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue</title><author>Mikus, Judith ; Steverding, Dietmar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-8131d2a40304a8701b5fa7ae679871cb6b1c10520e90084114e0c5e9f39e563d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alamar Blue</topic><topic>Amphotericin B - pharmacology</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimony Sodium Gluconate - pharmacology</topic><topic>Antiprotozoal Agents - pharmacology</topic><topic>Colorimetry</topic><topic>Colorimetry - methods</topic><topic>Coloring Agents - chemistry</topic><topic>Drug sensitivity</topic><topic>In vitro assay</topic><topic>Leishmania major</topic><topic>Leishmania major - chemistry</topic><topic>Leishmania major - drug effects</topic><topic>Leishmaniasis, Cutaneous - drug therapy</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Oxazines</topic><topic>Oxidation-Reduction</topic><topic>Parasitic Sensitivity Tests - methods</topic><topic>Paromomycin - pharmacology</topic><topic>Reproducibility of Results</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mikus, Judith</creatorcontrib><creatorcontrib>Steverding, Dietmar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mikus, Judith</au><au>Steverding, Dietmar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue</atitle><jtitle>Parasitology international</jtitle><addtitle>Parasitol Int</addtitle><date>2000</date><risdate>2000</risdate><volume>48</volume><issue>3</issue><spage>265</spage><epage>269</epage><pages>265-269</pages><issn>1383-5769</issn><eissn>1873-0329</eissn><abstract>A quantitative colorimetric assay using the oxidation–reduction indicator Alamar Blue® was developed to measure cytotoxicity of compounds against the protozoan parasite
Leishmania. Absorbance increased linearly with the plating density of promastigotes of
L. major MRHO/IR/76 vaccine strain up to at least 2.5×10
6 cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue®. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue® assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>11227767</pmid><doi>10.1016/S1383-5769(99)00020-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1383-5769 |
| ispartof | Parasitology international, 2000, Vol.48 (3), p.265-269 |
| issn | 1383-5769 1873-0329 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alamar Blue Amphotericin B - pharmacology Animals Anti-Bacterial Agents - pharmacology Antimony Sodium Gluconate - pharmacology Antiprotozoal Agents - pharmacology Colorimetry Colorimetry - methods Coloring Agents - chemistry Drug sensitivity In vitro assay Leishmania major Leishmania major - chemistry Leishmania major - drug effects Leishmaniasis, Cutaneous - drug therapy Leishmaniasis, Cutaneous - parasitology Oxazines Oxidation-Reduction Parasitic Sensitivity Tests - methods Paromomycin - pharmacology Reproducibility of Results Xanthenes |
| title | A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue |
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