Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911
The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of M r 11,500, ORFA, is synthesized from an upstream reading frame. A l...
Gespeichert in:
Veröffentlicht in: | Journal of molecular biology 1991-12, Vol.222 (3), p.465-477 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 477 |
---|---|
container_issue | 3 |
container_start_page | 465 |
container_title | Journal of molecular biology |
container_volume | 222 |
creator | Polard, P. Prère, M.F. Chandler, M. Fayet, O. |
description | The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of
M
r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between
orfA and a downstream frame,
orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The
orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the
orfB phase whereas the second appears to initiate in the
orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation
in vivo. The
orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase. |
doi_str_mv | 10.1016/0022-2836(91)90490-W |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72562740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>002228369190490W</els_id><sourcerecordid>72562740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-650ec3d68d1baa861cc228bd11936a73383b656cb53f1efd6c53ee090338a1763</originalsourceid><addsrcrecordid>eNp9kF1rFDEUhoModVv9Bwq5EKkXo_nYySQ3QilqC4UKuvQyZJIza2Q2s-ZkRW_87c3sLu2dVyHnfc6b8BDyirP3nHH1gTEhGqGlOjf8nWFLw5q7J2TBmTaNVlI_JYsH5Dk5RfzJGGvlUp-QE64UM0IuyL-veVpnt9lAoCW7hKMrcUpupEOdAv6IQ4lpTV0KNKZY4j6mrtQJvVitqJ9CvcdE15CAwp9tBsQZmQbaO18gx1oWE0LebyL82kHyQK-_Gc5fkGeDGxFeHs8zsvr86fvlVXNz--X68uKm8VKr0qiWgZdB6cB757Ti3guh-8C5kcp1UmrZq1b5vpUDhyEo30oAZlhNHO-UPCNvD73bPNX3sdhNRA_j6BJMO7SdaJXolqyCywPo84SYYbDbHDcu_7Wc2Vm7nZ3a2ak13O6127u69vrYv-uryselg-eavznmDr0bq9vkIz5gLa99oqvYxwMG1cXvCNmij7OtEDP4YsMU__-PewVBn5M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72562740</pqid></control><display><type>article</type><title>Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Polard, P. ; Prère, M.F. ; Chandler, M. ; Fayet, O.</creator><creatorcontrib>Polard, P. ; Prère, M.F. ; Chandler, M. ; Fayet, O.</creatorcontrib><description>The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of
M
r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between
orfA and a downstream frame,
orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The
orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the
orfB phase whereas the second appears to initiate in the
orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation
in vivo. The
orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(91)90490-W</identifier><identifier>PMID: 1660923</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; AUU initiation ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Codon - genetics ; DNA Transposable Elements - genetics ; Enterobacteriaceae - genetics ; Escherichia coli - genetics ; Frameshift Mutation - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Genetic Complementation Test ; insertion sequence ; IS3 family ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Conformation ; Peptide Chain Initiation, Translational - genetics ; Protein Biosynthesis - genetics ; Reading Frames - genetics ; Repetitive Sequences, Nucleic Acid - genetics ; Shigella dysenteriae - genetics ; Translation. Translation factors. Protein processing ; translational frameshifting ; transposition</subject><ispartof>Journal of molecular biology, 1991-12, Vol.222 (3), p.465-477</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-650ec3d68d1baa861cc228bd11936a73383b656cb53f1efd6c53ee090338a1763</citedby><cites>FETCH-LOGICAL-c386t-650ec3d68d1baa861cc228bd11936a73383b656cb53f1efd6c53ee090338a1763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2836(91)90490-W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5183627$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1660923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Polard, P.</creatorcontrib><creatorcontrib>Prère, M.F.</creatorcontrib><creatorcontrib>Chandler, M.</creatorcontrib><creatorcontrib>Fayet, O.</creatorcontrib><title>Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of
M
r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between
orfA and a downstream frame,
orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The
orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the
orfB phase whereas the second appears to initiate in the
orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation
in vivo. The
orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.</description><subject>Amino Acid Sequence</subject><subject>AUU initiation</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Codon - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>Enterobacteriaceae - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Frameshift Mutation - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Genetic Complementation Test</subject><subject>insertion sequence</subject><subject>IS3 family</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Peptide Chain Initiation, Translational - genetics</subject><subject>Protein Biosynthesis - genetics</subject><subject>Reading Frames - genetics</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Shigella dysenteriae - genetics</subject><subject>Translation. Translation factors. Protein processing</subject><subject>translational frameshifting</subject><subject>transposition</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoModVv9Bwq5EKkXo_nYySQ3QilqC4UKuvQyZJIza2Q2s-ZkRW_87c3sLu2dVyHnfc6b8BDyirP3nHH1gTEhGqGlOjf8nWFLw5q7J2TBmTaNVlI_JYsH5Dk5RfzJGGvlUp-QE64UM0IuyL-veVpnt9lAoCW7hKMrcUpupEOdAv6IQ4lpTV0KNKZY4j6mrtQJvVitqJ9CvcdE15CAwp9tBsQZmQbaO18gx1oWE0LebyL82kHyQK-_Gc5fkGeDGxFeHs8zsvr86fvlVXNz--X68uKm8VKr0qiWgZdB6cB757Ti3guh-8C5kcp1UmrZq1b5vpUDhyEo30oAZlhNHO-UPCNvD73bPNX3sdhNRA_j6BJMO7SdaJXolqyCywPo84SYYbDbHDcu_7Wc2Vm7nZ3a2ak13O6127u69vrYv-uryselg-eavznmDr0bq9vkIz5gLa99oqvYxwMG1cXvCNmij7OtEDP4YsMU__-PewVBn5M</recordid><startdate>19911205</startdate><enddate>19911205</enddate><creator>Polard, P.</creator><creator>Prère, M.F.</creator><creator>Chandler, M.</creator><creator>Fayet, O.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911205</creationdate><title>Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911</title><author>Polard, P. ; Prère, M.F. ; Chandler, M. ; Fayet, O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-650ec3d68d1baa861cc228bd11936a73383b656cb53f1efd6c53ee090338a1763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>AUU initiation</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Codon - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>Enterobacteriaceae - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Frameshift Mutation - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>Genetic Complementation Test</topic><topic>insertion sequence</topic><topic>IS3 family</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Peptide Chain Initiation, Translational - genetics</topic><topic>Protein Biosynthesis - genetics</topic><topic>Reading Frames - genetics</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Shigella dysenteriae - genetics</topic><topic>Translation. Translation factors. Protein processing</topic><topic>translational frameshifting</topic><topic>transposition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polard, P.</creatorcontrib><creatorcontrib>Prère, M.F.</creatorcontrib><creatorcontrib>Chandler, M.</creatorcontrib><creatorcontrib>Fayet, O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polard, P.</au><au>Prère, M.F.</au><au>Chandler, M.</au><au>Fayet, O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1991-12-05</date><risdate>1991</risdate><volume>222</volume><issue>3</issue><spage>465</spage><epage>477</epage><pages>465-477</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of
M
r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between
orfA and a downstream frame,
orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The
orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the
orfB phase whereas the second appears to initiate in the
orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation
in vivo. The
orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1660923</pmid><doi>10.1016/0022-2836(91)90490-W</doi><tpages>13</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0022-2836 |
ispartof | Journal of molecular biology, 1991-12, Vol.222 (3), p.465-477 |
issn | 0022-2836 1089-8638 |
language | eng |
recordid | cdi_proquest_miscellaneous_72562740 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Amino Acid Sequence AUU initiation Base Sequence Biological and medical sciences Cloning, Molecular Codon - genetics DNA Transposable Elements - genetics Enterobacteriaceae - genetics Escherichia coli - genetics Frameshift Mutation - genetics Fundamental and applied biological sciences. Psychology Gene Expression - genetics Genetic Complementation Test insertion sequence IS3 family Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation Peptide Chain Initiation, Translational - genetics Protein Biosynthesis - genetics Reading Frames - genetics Repetitive Sequences, Nucleic Acid - genetics Shigella dysenteriae - genetics Translation. Translation factors. Protein processing translational frameshifting transposition |
title | Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911 |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T02%3A33%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Programmed%20translational%20frameshifting%20and%20initiation%20at%20an%20AUU%20codon%20in%20gene%20expression%20of%20bacterial%20insertion%20sequence%20IS911&rft.jtitle=Journal%20of%20molecular%20biology&rft.au=Polard,%20P.&rft.date=1991-12-05&rft.volume=222&rft.issue=3&rft.spage=465&rft.epage=477&rft.pages=465-477&rft.issn=0022-2836&rft.eissn=1089-8638&rft.coden=JMOBAK&rft_id=info:doi/10.1016/0022-2836(91)90490-W&rft_dat=%3Cproquest_cross%3E72562740%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72562740&rft_id=info:pmid/1660923&rft_els_id=002228369190490W&rfr_iscdi=true |