Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911

The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of M r 11,500, ORFA, is synthesized from an upstream reading frame. A l...

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Veröffentlicht in:Journal of molecular biology 1991-12, Vol.222 (3), p.465-477
Hauptverfasser: Polard, P., Prère, M.F., Chandler, M., Fayet, O.
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container_end_page 477
container_issue 3
container_start_page 465
container_title Journal of molecular biology
container_volume 222
creator Polard, P.
Prère, M.F.
Chandler, M.
Fayet, O.
description The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of M r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between orfA and a downstream frame, orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the orfB phase whereas the second appears to initiate in the orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation in vivo. The orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.
doi_str_mv 10.1016/0022-2836(91)90490-W
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Psychology ; Gene Expression - genetics ; Genetic Complementation Test ; insertion sequence ; IS3 family ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Conformation ; Peptide Chain Initiation, Translational - genetics ; Protein Biosynthesis - genetics ; Reading Frames - genetics ; Repetitive Sequences, Nucleic Acid - genetics ; Shigella dysenteriae - genetics ; Translation. Translation factors. 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The results indicate that three major species are produced from two consecutive reading frames. A protein of M r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between orfA and a downstream frame, orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the orfB phase whereas the second appears to initiate in the orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation in vivo. The orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.</description><subject>Amino Acid Sequence</subject><subject>AUU initiation</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Codon - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>Enterobacteriaceae - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Frameshift Mutation - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Genetic Complementation Test</subject><subject>insertion sequence</subject><subject>IS3 family</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Peptide Chain Initiation, Translational - genetics</subject><subject>Protein Biosynthesis - genetics</subject><subject>Reading Frames - genetics</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Shigella dysenteriae - genetics</subject><subject>Translation. Translation factors. 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Psychology</topic><topic>Gene Expression - genetics</topic><topic>Genetic Complementation Test</topic><topic>insertion sequence</topic><topic>IS3 family</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Peptide Chain Initiation, Translational - genetics</topic><topic>Protein Biosynthesis - genetics</topic><topic>Reading Frames - genetics</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Shigella dysenteriae - genetics</topic><topic>Translation. Translation factors. Protein processing</topic><topic>translational frameshifting</topic><topic>transposition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polard, P.</creatorcontrib><creatorcontrib>Prère, M.F.</creatorcontrib><creatorcontrib>Chandler, M.</creatorcontrib><creatorcontrib>Fayet, O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polard, P.</au><au>Prère, M.F.</au><au>Chandler, M.</au><au>Fayet, O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1991-12-05</date><risdate>1991</risdate><volume>222</volume><issue>3</issue><spage>465</spage><epage>477</epage><pages>465-477</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>The proteins expressed by insertion sequence IS911, a member of the widespread IS3 family of elements, have been analyzed. The results indicate that three major species are produced from two consecutive reading frames. A protein of M r 11,500, ORFA, is synthesized from an upstream reading frame. A larger protein, ORFAB, uses the same initiation codon and is produced by a −1 programmed translational frameshift between orfA and a downstream frame, orfB, whose amino acid sequence shows significant homology with retroviral integrase proteins. The orfB frame is also expressed independently in two alternative forms: the first uses a rare AUU initiation codon in the orfB phase whereas the second appears to initiate in the orfA phase and is produced by a −1 frameshift mechanism similar to that used in ORFAB expression. A specific IS911 integration reaction using a minimal active junction composed of 51 base-pairs of the right inverted repeat and a flanking phage λ sequence resembling a second end in inverted orientation has been developed to analyze the functions of these proteins by transcomplementation in vivo. The orfA and orfB frames are shown to be essential and production of ORFAB is shown to stimulate integration in this system, suggesting that this fusion protein is the IS911 transposase.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1660923</pmid><doi>10.1016/0022-2836(91)90490-W</doi><tpages>13</tpages></addata></record>
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subjects Amino Acid Sequence
AUU initiation
Base Sequence
Biological and medical sciences
Cloning, Molecular
Codon - genetics
DNA Transposable Elements - genetics
Enterobacteriaceae - genetics
Escherichia coli - genetics
Frameshift Mutation - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression - genetics
Genetic Complementation Test
insertion sequence
IS3 family
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
Peptide Chain Initiation, Translational - genetics
Protein Biosynthesis - genetics
Reading Frames - genetics
Repetitive Sequences, Nucleic Acid - genetics
Shigella dysenteriae - genetics
Translation. Translation factors. Protein processing
translational frameshifting
transposition
title Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911
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