Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13

Incorporation of numerous copies of a heterologous protein (bovine pancreatic trypsin inhibitor; BPTI) fused to the mature major coat protein (gene VII product; VIII) of bacteriophage M13 has been demonstrated. Optimization of the promoter, signal peptide and host bacterial strain allowed for the co...

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Veröffentlicht in:	Gene 1991-12, Vol.109 (1), p.13-19
Hauptverfasser:	Markland, W., Roberts, B.L., Saxena, M.J., Guterman, S.K., Ladner, R.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Aprotinin - genetics Base Sequence Biological and medical sciences Biotechnology Bovine pancreatic trypsin inhibitor-VIII protein fusion Capsid - genetics Coliphages - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Genetic engineering Genetic technics Genetic Vectors - genetics Methods. Procedures. Technologies Molecular Sequence Data Promoter Regions, Genetic - genetics Protein Processing, Post-Translational Protein Sorting Signals - genetics recombinant DNA Recombinant Fusion Proteins - genetics single-stranded DNA phage Vectors (cloning, transfer, expression). Insertion sequences and transposons
Online-Zugang:	Volltext
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