Coatomer Vesicles are not Required for Inhibition of Golgi Transport by G‐Protein Activators

The G‐protein activators guanosine 5′‐O‐(3‐thiodiphosphate) (GTPΓS) and aluminum fluoride (AlF) are thought to inhibit transport between Golgi cisternae by causing the accumulation of nonfunctional coatomer‐coated transport vesicles on the Golgi. Although GTPΓS and AlF inhibit transport in cell‐free intra‐Golgi transport systems, blocking coatomer vesicle formation does not. We therefore determined whether inhibition of in vitro Golgi transport by these agents requires coatomer vesicle formation. Depletion of coatomer was found to completely block coated vesicle formation on Golgi cisternae without affecting inhibition of in vitro transport by either GTPΓS or AlF. Depletion of ADP‐ribosylation factor (ARF) prevented inhibition of transport by GTPΓS, but not by AlF, suggesting that the AlF‐sensitive component in transport may not be a GTP‐binding protein. Surprisingly, depletion of cytosolic ARF did not prevent the GTPΓS‐induced formation of Golgi‐coated vesicles, whereas ARF was required for AlF‐induced vesicle formation. Although ARF or coatomer depletion caused an increase in the fenestration of cisternae, no other utrastructural changes were observed that might explain the inhibition of transport by GTPΓS or AlF. These findings suggest that ARF‐GTPΓS and AlF act by distinct and coatomer‐independent mechanisms to inhibit membrane fusion in cell‐free intra‐Golgi transport.