Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas
The cellular deoxyribonucleic acid content of formalin-fixed, paraffin-embedded tissue from nine renal oncocytomas was determined using an Epics-C flow cytometer. The tissues were all from surgical specimens, and all neoplasms met stringent light and electron microscopic criteria for the diagnosis o...
Gespeichert in:
| Veröffentlicht in: | Urology (Ridgewood, N.J.) N.J.), 1991-10, Vol.38 (4), p.310-313 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 313 |
|---|---|
| container_issue | 4 |
| container_start_page | 310 |
| container_title | Urology (Ridgewood, N.J.) |
| container_volume | 38 |
| creator | Psihramis, Kostantinos E Goldberg, Susan D |
| description | The cellular deoxyribonucleic acid content of formalin-fixed, paraffin-embedded tissue from nine renal oncocytomas was determined using an Epics-C flow cytometer. The tissues were all from surgical specimens, and all neoplasms met stringent light and electron microscopic criteria for the diagnosis of oncocytoma. Thirteen samples of pure tumor, 5 of tumor and normal kidney, and 3 of normal kidney alone were analyzed. All oncocytomas had single, sharp GI peaks with no evidence of aneuploidy, and this peak was similar to that of normal renal parenchyma. This was true even for three oncocytomas that had multiple major chromosomal anomalies on karyotyping. These data support the concept that renal oncocytomas are euploid neoplasms, consistent with their benign biologic behavior. The apparent discrepancy between the results of flow cytometry and karyotyping is discussed. |
| doi_str_mv | 10.1016/0090-4295(91)80141-S |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72560835</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>009042959180141S</els_id><sourcerecordid>72560835</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-e1bc7f1a1c3ee4b63983d7c4261d028725dd4db17a0a654fd63e1c9cd667dca33</originalsourceid><addsrcrecordid>eNp9kcFO3DAQhq0KRBfaN2ilHFAFh1BPnDjxBalCUJCQOEBvlSxnPJFcZW2wk9J9exx2BbeefJjv_zX-hrEvwM-Ag_zOueJlXanmRMFpx6GG8v4DW0FTtaVSqtljqzfkIztM6Q_nXErZHrADaJsGhFyx31djeC5wM4U1TdFhYbwZN8mlIgwF0jjOo4mFpfBvE10f_IwjLRQ6W2DwE_lpIb3zVETK2SJ4DK99Jn1i-4MZE33evUfs19Xlw8V1eXv38-bix22JgsNUEvTYDmAABVHdS6E6YVusKwmWV11bNdbWtofWcCOberBSEKBCm_9i0QhxxL5tex9jeJopTXrt0rK88RTmpHOD5J1oMlhvQYwhpUiDfoxubeJGA9eLVL0Y04sxrUC_StX3OfZ11z_3a7Lvoa3FPD_ezU1CMw7ReHTpDcsHUdBBxs63GGUXfx1FndCRR7IuEk7aBvf_PV4AT8WUxg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72560835</pqid></control><display><type>article</type><title>Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Psihramis, Kostantinos E ; Goldberg, Susan D</creator><creatorcontrib>Psihramis, Kostantinos E ; Goldberg, Susan D</creatorcontrib><description>The cellular deoxyribonucleic acid content of formalin-fixed, paraffin-embedded tissue from nine renal oncocytomas was determined using an Epics-C flow cytometer. The tissues were all from surgical specimens, and all neoplasms met stringent light and electron microscopic criteria for the diagnosis of oncocytoma. Thirteen samples of pure tumor, 5 of tumor and normal kidney, and 3 of normal kidney alone were analyzed. All oncocytomas had single, sharp GI peaks with no evidence of aneuploidy, and this peak was similar to that of normal renal parenchyma. This was true even for three oncocytomas that had multiple major chromosomal anomalies on karyotyping. These data support the concept that renal oncocytomas are euploid neoplasms, consistent with their benign biologic behavior. The apparent discrepancy between the results of flow cytometry and karyotyping is discussed.</description><identifier>ISSN: 0090-4295</identifier><identifier>EISSN: 1527-9995</identifier><identifier>DOI: 10.1016/0090-4295(91)80141-S</identifier><identifier>PMID: 1755136</identifier><identifier>CODEN: URGYAZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adenoma - genetics ; Aged ; Aged, 80 and over ; Biological and medical sciences ; DNA, Neoplasm - analysis ; Female ; Flow Cytometry ; Humans ; Karyotyping ; Kidney Neoplasms - genetics ; Kidneys ; Male ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Ploidies ; Tumors of the urinary system</subject><ispartof>Urology (Ridgewood, N.J.), 1991-10, Vol.38 (4), p.310-313</ispartof><rights>1991 Cahners Publishing Company</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-e1bc7f1a1c3ee4b63983d7c4261d028725dd4db17a0a654fd63e1c9cd667dca33</citedby><cites>FETCH-LOGICAL-c301t-e1bc7f1a1c3ee4b63983d7c4261d028725dd4db17a0a654fd63e1c9cd667dca33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0090-4295(91)80141-S$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5279181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1755136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Psihramis, Kostantinos E</creatorcontrib><creatorcontrib>Goldberg, Susan D</creatorcontrib><title>Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas</title><title>Urology (Ridgewood, N.J.)</title><addtitle>Urology</addtitle><description>The cellular deoxyribonucleic acid content of formalin-fixed, paraffin-embedded tissue from nine renal oncocytomas was determined using an Epics-C flow cytometer. The tissues were all from surgical specimens, and all neoplasms met stringent light and electron microscopic criteria for the diagnosis of oncocytoma. Thirteen samples of pure tumor, 5 of tumor and normal kidney, and 3 of normal kidney alone were analyzed. All oncocytomas had single, sharp GI peaks with no evidence of aneuploidy, and this peak was similar to that of normal renal parenchyma. This was true even for three oncocytomas that had multiple major chromosomal anomalies on karyotyping. These data support the concept that renal oncocytomas are euploid neoplasms, consistent with their benign biologic behavior. The apparent discrepancy between the results of flow cytometry and karyotyping is discussed.</description><subject>Adenoma - genetics</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>DNA, Neoplasm - analysis</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidneys</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Ploidies</subject><subject>Tumors of the urinary system</subject><issn>0090-4295</issn><issn>1527-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFO3DAQhq0KRBfaN2ilHFAFh1BPnDjxBalCUJCQOEBvlSxnPJFcZW2wk9J9exx2BbeefJjv_zX-hrEvwM-Ag_zOueJlXanmRMFpx6GG8v4DW0FTtaVSqtljqzfkIztM6Q_nXErZHrADaJsGhFyx31djeC5wM4U1TdFhYbwZN8mlIgwF0jjOo4mFpfBvE10f_IwjLRQ6W2DwE_lpIb3zVETK2SJ4DK99Jn1i-4MZE33evUfs19Xlw8V1eXv38-bix22JgsNUEvTYDmAABVHdS6E6YVusKwmWV11bNdbWtofWcCOberBSEKBCm_9i0QhxxL5tex9jeJopTXrt0rK88RTmpHOD5J1oMlhvQYwhpUiDfoxubeJGA9eLVL0Y04sxrUC_StX3OfZ11z_3a7Lvoa3FPD_ezU1CMw7ReHTpDcsHUdBBxs63GGUXfx1FndCRR7IuEk7aBvf_PV4AT8WUxg</recordid><startdate>199110</startdate><enddate>199110</enddate><creator>Psihramis, Kostantinos E</creator><creator>Goldberg, Susan D</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199110</creationdate><title>Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas</title><author>Psihramis, Kostantinos E ; Goldberg, Susan D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-e1bc7f1a1c3ee4b63983d7c4261d028725dd4db17a0a654fd63e1c9cd667dca33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adenoma - genetics</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>DNA, Neoplasm - analysis</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Kidney Neoplasms - genetics</topic><topic>Kidneys</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Ploidies</topic><topic>Tumors of the urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Psihramis, Kostantinos E</creatorcontrib><creatorcontrib>Goldberg, Susan D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Urology (Ridgewood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Psihramis, Kostantinos E</au><au>Goldberg, Susan D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas</atitle><jtitle>Urology (Ridgewood, N.J.)</jtitle><addtitle>Urology</addtitle><date>1991-10</date><risdate>1991</risdate><volume>38</volume><issue>4</issue><spage>310</spage><epage>313</epage><pages>310-313</pages><issn>0090-4295</issn><eissn>1527-9995</eissn><coden>URGYAZ</coden><abstract>The cellular deoxyribonucleic acid content of formalin-fixed, paraffin-embedded tissue from nine renal oncocytomas was determined using an Epics-C flow cytometer. The tissues were all from surgical specimens, and all neoplasms met stringent light and electron microscopic criteria for the diagnosis of oncocytoma. Thirteen samples of pure tumor, 5 of tumor and normal kidney, and 3 of normal kidney alone were analyzed. All oncocytomas had single, sharp GI peaks with no evidence of aneuploidy, and this peak was similar to that of normal renal parenchyma. This was true even for three oncocytomas that had multiple major chromosomal anomalies on karyotyping. These data support the concept that renal oncocytomas are euploid neoplasms, consistent with their benign biologic behavior. The apparent discrepancy between the results of flow cytometry and karyotyping is discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>1755136</pmid><doi>10.1016/0090-4295(91)80141-S</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-4295 |
| ispartof | Urology (Ridgewood, N.J.), 1991-10, Vol.38 (4), p.310-313 |
| issn | 0090-4295 1527-9995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72560835 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Adenoma - genetics Aged Aged, 80 and over Biological and medical sciences DNA, Neoplasm - analysis Female Flow Cytometry Humans Karyotyping Kidney Neoplasms - genetics Kidneys Male Medical sciences Middle Aged Nephrology. Urinary tract diseases Ploidies Tumors of the urinary system |
| title | Flow cytometric analysis of cellular deoxyribonucleic acid content of nine renal oncocytomas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T08%3A37%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Flow%20cytometric%20analysis%20of%20cellular%20deoxyribonucleic%20acid%20content%20of%20nine%20renal%20oncocytomas&rft.jtitle=Urology%20(Ridgewood,%20N.J.)&rft.au=Psihramis,%20Kostantinos%20E&rft.date=1991-10&rft.volume=38&rft.issue=4&rft.spage=310&rft.epage=313&rft.pages=310-313&rft.issn=0090-4295&rft.eissn=1527-9995&rft.coden=URGYAZ&rft_id=info:doi/10.1016/0090-4295(91)80141-S&rft_dat=%3Cproquest_cross%3E72560835%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72560835&rft_id=info:pmid/1755136&rft_els_id=009042959180141S&rfr_iscdi=true |