Reconstitution and properties of homologous and chimeric HIV-1.HIV-2 p66.p51 reverse transcriptase

Metal chelate affinity chromatography has been used to follow reconstitution of the 66- and 51-kDa human immunodeficiency (HIV)-1 and HIV-2 reverse transcriptase (RT) subunits into heterodimer, as well as chimeric enzymes comprised of heterologous subunits. By adding a small N-terminal polyhistidine...

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Veröffentlicht in:	The Journal of biological chemistry 1991-12, Vol.266 (34), p.23003-23009
Hauptverfasser:	HOWARD, K. J, FRANK, K. B, SIM, I. S, LE GRICE, S. F. J
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Analytical, structural and metabolic biochemistry Biological and medical sciences Chimera Chromatography, Gel Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Escherichia coli - genetics Fundamental and applied biological sciences. Psychology HIV Reverse Transcriptase HIV-1 - enzymology HIV-2 - enzymology Protein Multimerization Reverse Transcriptase Inhibitors RNA-Directed DNA Polymerase - isolation & purification RNA-Directed DNA Polymerase - metabolism Transferases
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