In situ hybridization in wholemounted lamprey spinal cord: localization of netrin mRNA expression

The lamprey has been used as a model for the study of vertebrate neuronal circuitry and spinal cord regeneration. One of the advantages of this preparation is the ability to view the entire CNS in wholemount, including several identified neurons and neuron groups. However, because of difficulties in...

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Veröffentlicht in:	Journal of neuroscience methods 2000-12, Vol.104 (1), p.19-25
Hauptverfasser:	Shifman, Michael I, Selzer, Michael E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Axon guidance Biological and medical sciences Development. Senescence. Regeneration. Transplantation Fundamental and applied biological sciences. Psychology In Situ Hybridization In Vitro Techniques Lamprey Lampreys - anatomy & histology Lampreys - metabolism Meninx primitiva Nerve Growth Factors - genetics Netrin-1 Neurons - chemistry Neurons - cytology Regeneration RNA Probes - pharmacology RNA, Messenger - analysis Spinal cord Spinal Cord - chemistry Spinal Cord - cytology Tumor Suppressor Proteins Vertebrates: nervous system and sense organs Wholemount in situ hybridization
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