Stability study of azure B, eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography
The stability of Azure B and Eosin Y in stock solutions of the individual compounds as well as in mixtures of the two dyes was studied. The purpose of the study of these two essential constituents of the Romanowsky Giemsa stain, commonly used in cytology and histology, was to select a stable mixture...
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| Veröffentlicht in: | The Histochemical journal 1991-04, Vol.23 (4), p.189-195 |
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| container_end_page | 195 |
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| container_issue | 4 |
| container_start_page | 189 |
| container_title | The Histochemical journal |
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| creator | VAN LIEDEKERKE, B. M NELIS, H. J WITTEKIND, D. H DE LEENHEER, A. P |
| description | The stability of Azure B and Eosin Y in stock solutions of the individual compounds as well as in mixtures of the two dyes was studied. The purpose of the study of these two essential constituents of the Romanowsky Giemsa stain, commonly used in cytology and histology, was to select a stable mixture as a definitive stock solution. Two specific high performance liquid chromatographic methods were used to monitor qualitative and quantitative changes in solutions. Several parameters influencing the stability of Azure B were examined e.g. the type of counter ion, the presence of Eosin Y and the type of solvent used. The second part focused on the stability of Eosin Y in mixtures with different cationic dyes submitted to high temperatures. In conclusion, an Azure B SCN-Eosin Y acid mixture in dimethylsulphoxide (concentrations 0.75% and 0.12%, respectively) was selected as being the most appropriate composition of a stock solution for the Romanowsky Giemsa stain. |
| doi_str_mv | 10.1007/BF01046590 |
| format | Article |
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In conclusion, an Azure B SCN-Eosin Y acid mixture in dimethylsulphoxide (concentrations 0.75% and 0.12%, respectively) was selected as being the most appropriate composition of a stock solution for the Romanowsky Giemsa stain.</description><identifier>ISSN: 0018-2214</identifier><identifier>EISSN: 1573-6865</identifier><identifier>DOI: 10.1007/BF01046590</identifier><identifier>PMID: 1721046</identifier><identifier>CODEN: HISJAE</identifier><language>eng</language><publisher>London: Kluwer</publisher><subject>Azure Stains - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Diverse techniques ; Drug Stability ; Eosine Yellowish-(YS) - chemistry ; Fundamental and applied biological sciences. 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Several parameters influencing the stability of Azure B were examined e.g. the type of counter ion, the presence of Eosin Y and the type of solvent used. The second part focused on the stability of Eosin Y in mixtures with different cationic dyes submitted to high temperatures. In conclusion, an Azure B SCN-Eosin Y acid mixture in dimethylsulphoxide (concentrations 0.75% and 0.12%, respectively) was selected as being the most appropriate composition of a stock solution for the Romanowsky Giemsa stain.</description><subject>Azure Stains - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diverse techniques</subject><subject>Drug Stability</subject><subject>Eosine Yellowish-(YS) - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Solutions</subject><subject>Solvents</subject><subject>Staining and Labeling</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtv2zAURomigeskXboX4NIOQZXwIT48NkGcFggQIE2GTMIVRdqsJdEhJQTqln9eGjbg6Q73nDN8CH2h5JISoq6ul4SSUooF-YDmVCheSC3FRzQnhOqCMVp-Qqcp_SWELJSSMzSjiu2MOXr_M0DtWz9MOA1jM-HgMPwbo8XXP7ANyff4BUPfYBO6zkbjocWPoYM-vKXNhO-87RJkNZgNTqEdBx_6hMfsrfDar9Z4a6MLMQvG4ta_jj6n1jEXhrCKsF1P5-jEQZvs58M9Q8_L26ebX8X9w93vm5_3heGUDYV1tZUajAQiiAPpqG5s7bg0pVQ1p7UjRjtoSqqFYwKodkpztxDMOgZa8TP0fd_dxvA62jRUnU_Gti30NoypUkwIrgTL4MUeNDGkFK2rttF3EKeKkmq3d3XcO8NfD9Wx7mxzRPcD5_-3wx-SgdbFPIRPR2whBS-p5P8B7TCKVw</recordid><startdate>19910401</startdate><enddate>19910401</enddate><creator>VAN LIEDEKERKE, B. M</creator><creator>NELIS, H. J</creator><creator>WITTEKIND, D. 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P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability study of azure B, eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography</atitle><jtitle>The Histochemical journal</jtitle><addtitle>Histochem J</addtitle><date>1991-04-01</date><risdate>1991</risdate><volume>23</volume><issue>4</issue><spage>189</spage><epage>195</epage><pages>189-195</pages><issn>0018-2214</issn><eissn>1573-6865</eissn><coden>HISJAE</coden><abstract>The stability of Azure B and Eosin Y in stock solutions of the individual compounds as well as in mixtures of the two dyes was studied. The purpose of the study of these two essential constituents of the Romanowsky Giemsa stain, commonly used in cytology and histology, was to select a stable mixture as a definitive stock solution. Two specific high performance liquid chromatographic methods were used to monitor qualitative and quantitative changes in solutions. Several parameters influencing the stability of Azure B were examined e.g. the type of counter ion, the presence of Eosin Y and the type of solvent used. The second part focused on the stability of Eosin Y in mixtures with different cationic dyes submitted to high temperatures. In conclusion, an Azure B SCN-Eosin Y acid mixture in dimethylsulphoxide (concentrations 0.75% and 0.12%, respectively) was selected as being the most appropriate composition of a stock solution for the Romanowsky Giemsa stain.</abstract><cop>London</cop><pub>Kluwer</pub><pmid>1721046</pmid><doi>10.1007/BF01046590</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Histochemical journal, 1991-04, Vol.23 (4), p.189-195 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Azure Stains - chemistry Biological and medical sciences Chromatography, High Pressure Liquid Diverse techniques Drug Stability Eosine Yellowish-(YS) - chemistry Fundamental and applied biological sciences. Psychology Molecular and cellular biology Solutions Solvents Staining and Labeling |
| title | Stability study of azure B, eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography |
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