Genetic variability in the Iberian imperial eagle (Aquila adalberti) demonstrated by RAPD analysis
RAPD analysis was used to estimate the genetic diversity in an Iberian imperial eagle (Aquila adalberti) population, one of the most threatened bird species in the world. Forty-five of 60 arbitrarily designed primers amplified 614 loci in 25 individual eagles, 59.7% of which were polymorphic. In con...
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Veröffentlicht in: | The Journal of heredity 2000-11, Vol.91 (6), p.495-499 |
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creator | Padilla, J A Martínez-Trancón, M Rabasco, A Parejo, J C Sansinforiano, M E Guijo, M I |
description | RAPD analysis was used to estimate the genetic diversity in an Iberian imperial eagle (Aquila adalberti) population, one of the most threatened bird species in the world. Forty-five of 60 arbitrarily designed primers amplified 614 loci in 25 individual eagles, 59.7% of which were polymorphic. In contrast to the traditional allozyme analysis performed in a previous study, the RAPD method has revealed a high level of heterozygosity in this species (H = 0.267+/-0.008). The genetic distances estimated between 25 eagles can serve to establish more adequate mating in order to preserve genetic variability. Conservation efforts being carried out in Spain in this species might be successful based on the results obtained in the present work. |
doi_str_mv | 10.1093/jhered/91.6.495 |
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Forty-five of 60 arbitrarily designed primers amplified 614 loci in 25 individual eagles, 59.7% of which were polymorphic. In contrast to the traditional allozyme analysis performed in a previous study, the RAPD method has revealed a high level of heterozygosity in this species (H = 0.267+/-0.008). The genetic distances estimated between 25 eagles can serve to establish more adequate mating in order to preserve genetic variability. Conservation efforts being carried out in Spain in this species might be successful based on the results obtained in the present work.</description><identifier>ISSN: 0022-1503</identifier><identifier>EISSN: 1465-7333</identifier><identifier>DOI: 10.1093/jhered/91.6.495</identifier><identifier>PMID: 11218090</identifier><identifier>CODEN: JOHEA8</identifier><language>eng</language><publisher>United States: Oxford Publishing Limited (England)</publisher><subject>Animals ; Birds ; Deoxyribonucleic acid ; DNA ; Eagles - classification ; Eagles - genetics ; Female ; Genetic Variation ; Genetics ; Male ; Random Amplified Polymorphic DNA Technique</subject><ispartof>The Journal of heredity, 2000-11, Vol.91 (6), p.495-499</ispartof><rights>Copyright Oxford University Press(England) Nov/Dec 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11218090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Padilla, J A</creatorcontrib><creatorcontrib>Martínez-Trancón, M</creatorcontrib><creatorcontrib>Rabasco, A</creatorcontrib><creatorcontrib>Parejo, J C</creatorcontrib><creatorcontrib>Sansinforiano, M E</creatorcontrib><creatorcontrib>Guijo, M I</creatorcontrib><title>Genetic variability in the Iberian imperial eagle (Aquila adalberti) demonstrated by RAPD analysis</title><title>The Journal of heredity</title><addtitle>J Hered</addtitle><description>RAPD analysis was used to estimate the genetic diversity in an Iberian imperial eagle (Aquila adalberti) population, one of the most threatened bird species in the world. Forty-five of 60 arbitrarily designed primers amplified 614 loci in 25 individual eagles, 59.7% of which were polymorphic. In contrast to the traditional allozyme analysis performed in a previous study, the RAPD method has revealed a high level of heterozygosity in this species (H = 0.267+/-0.008). The genetic distances estimated between 25 eagles can serve to establish more adequate mating in order to preserve genetic variability. Conservation efforts being carried out in Spain in this species might be successful based on the results obtained in the present work.</description><subject>Animals</subject><subject>Birds</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Eagles - classification</subject><subject>Eagles - genetics</subject><subject>Female</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Male</subject><subject>Random Amplified Polymorphic DNA Technique</subject><issn>0022-1503</issn><issn>1465-7333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0MFLwzAUBvAgipvTszcJHkQP3fKSpk2OY-ocDBTRc0mWV5eRdlvTCvvvragXT-_x8ePx8Qi5BDYGpsVks8YG3UTDOBunWh6RIaSZTHIhxDEZMsZ5ApKJATmLccMYA6nZKRkAcFBMsyGxc6yx9Sv6aRpvrA--PVBf03aNdGGxz2rqq933Eiiaj4D0drrvfDDUOBN60fo76rDa1rFtTIuO2gN9nb7cU1ObcIg-npOT0oSIF79zRN4fH95mT8nyeb6YTZfJjou0TWyJXHMNMkUwXDhpMl0KC6kTUuRipXiuV6Uqc6ek5MAMMGaETRVakJmTYkRufu7umu2-w9gWlY8rDMHUuO1ikXOZqkxlPbz-BzfbrunbxgK0gkykivXo6hd1tkJX7BpfmeZQ_L1OfAHRNG_i</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Padilla, J A</creator><creator>Martínez-Trancón, M</creator><creator>Rabasco, A</creator><creator>Parejo, J C</creator><creator>Sansinforiano, M E</creator><creator>Guijo, M I</creator><general>Oxford Publishing Limited (England)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7SN</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20001101</creationdate><title>Genetic variability in the Iberian imperial eagle (Aquila adalberti) demonstrated by RAPD analysis</title><author>Padilla, J A ; Martínez-Trancón, M ; Rabasco, A ; Parejo, J C ; Sansinforiano, M E ; Guijo, M I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p234t-bfe2929154e1a23d5a69f3b14d35373c8279cf8f7d855210a100a3b48eb156d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Birds</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Eagles - classification</topic><topic>Eagles - genetics</topic><topic>Female</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Male</topic><topic>Random Amplified Polymorphic DNA Technique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Padilla, J A</creatorcontrib><creatorcontrib>Martínez-Trancón, M</creatorcontrib><creatorcontrib>Rabasco, A</creatorcontrib><creatorcontrib>Parejo, J C</creatorcontrib><creatorcontrib>Sansinforiano, M E</creatorcontrib><creatorcontrib>Guijo, M I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of heredity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padilla, J A</au><au>Martínez-Trancón, M</au><au>Rabasco, A</au><au>Parejo, J C</au><au>Sansinforiano, M E</au><au>Guijo, M I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variability in the Iberian imperial eagle (Aquila adalberti) demonstrated by RAPD analysis</atitle><jtitle>The Journal of heredity</jtitle><addtitle>J Hered</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>91</volume><issue>6</issue><spage>495</spage><epage>499</epage><pages>495-499</pages><issn>0022-1503</issn><eissn>1465-7333</eissn><coden>JOHEA8</coden><abstract>RAPD analysis was used to estimate the genetic diversity in an Iberian imperial eagle (Aquila adalberti) population, one of the most threatened bird species in the world. 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source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
subjects | Animals Birds Deoxyribonucleic acid DNA Eagles - classification Eagles - genetics Female Genetic Variation Genetics Male Random Amplified Polymorphic DNA Technique |
title | Genetic variability in the Iberian imperial eagle (Aquila adalberti) demonstrated by RAPD analysis |
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