alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure
MSH is a pituitary hormone derived by post-translational processing from POMC and involved in stress and background adaptation. N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH ac...
Gespeichert in:
| Veröffentlicht in: | Journal of endocrinology 2000-08, Vol.166 (2), p.427-435 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 435 |
|---|---|
| container_issue | 2 |
| container_start_page | 427 |
| container_title | Journal of endocrinology |
| container_volume | 166 |
| creator | Arends, RJ Rotllant, J Metz Mancera, JM Wendelaar Bonga, SE Flik, G |
| description | MSH is a pituitary hormone derived by post-translational processing from POMC and involved in stress and background adaptation. N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH acetylation in the sea bream (Sparus aurata L.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. Pituitary homogenates were purified by reversed-phase HPLC (RP-HPLC). The alpha-MSH content of fractions was measured by RIA. Immunoreactive RP-HPLC fractions were further analyzed by electrospray mass spectrometry and the peptide sequence determined as SYSMEHFRWGKPV-NH2. In the pituitary gland of sea bream, des-, mono- and diacetyl alpha-MSH were identified. Then plasma alpha-MSH levels were measured in sea bream adapted to different backgrounds. Surprisingly, we found the highest plasma alpha-MSH levels in white-adapted as compared with black-adapted sea bream with intermediate values for gray-adapted fish. This observation is in contrast with results that have been obtained in eel, trout or terrestrial vertebrates. Next, des-, mono- and diacetyl alpha-MSH forms were measured in homogenates of the pituitary gland and in plasma of sea bream exposed to air, to confinement, or to different backgrounds. Monoacetyl alpha-MSH was the predominant form in all control and experimental groups. The lowest content of monoacetyl alpha-MSH relative to des- and diacetyl alpha-MSH was found in white-adapted fish. Levels of des- and diacetyl alpha-MSH forms were similar under all conditions. We observed that monoacetyl alpha-MSH is the most abundant isoform in the pituitary gland after background adaptation, confinement and air exposure, in sea bream. These data indicate that the physiologically most potent isoform of alpha-MSH may vary from species to species. |
| doi_str_mv | 10.1677/joe.0.1660427 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72532137</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72532137</sourcerecordid><originalsourceid>FETCH-LOGICAL-b512t-8fe5d79bab1aa6b2208dc3cf249c7b4f089764a41782f8dd76477c82e2f0506b3</originalsourceid><addsrcrecordid>eNp9kUFv1DAQhS1ERZfCkSvyASGQmsV24jg5VhVQpEUcCudo7Ix3XZI42I6gv4M_3KRZCYRUTh7b37yx3yPkBWdbXir17sbjdilLVgj1iGx4oeqsrJh8TDaMCZExVctT8jTGG8a45Cp_Qk45q4Uqc7Ehv6EbD5B9vr6iYDDddpCcH6gbaDogHV2aXIJwS_cdDC319v44IlAdEHr65nqEMEUKU4AEdLd9u7QGjKMfItLkaeusxYBDohrM933w09DGc2r8YN2A_XKxKIMLFH-NPk4Bn5ETC13E58f1jHz78P7r5VW2-_Lx0-XFLtOSi5RVFmWrag2aA5RaCFa1JjdWFLVRurCsqlVZQMFVJWzVtvNGKVMJFJZJVur8jLxedcfgf0wYU9O7aLCbv4p-io0SMhc8VzOYraAJPsaAthmD62dbGs6aJYVmTqFZyvsUZv7lUXjSPbZ_0avtM_DqCEA00NkAg3HxDycFV1zOWLFiB7c__HQBG-18NG42zVln4MHx-dr2D_3_R98BNkmztg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72532137</pqid></control><display><type>article</type><title>alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Arends, RJ ; Rotllant, J ; Metz ; Mancera, JM ; Wendelaar Bonga, SE ; Flik, G</creator><creatorcontrib>Arends, RJ ; Rotllant, J ; Metz ; Mancera, JM ; Wendelaar Bonga, SE ; Flik, G ; Metz</creatorcontrib><description>MSH is a pituitary hormone derived by post-translational processing from POMC and involved in stress and background adaptation. N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH acetylation in the sea bream (Sparus aurata L.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. Pituitary homogenates were purified by reversed-phase HPLC (RP-HPLC). The alpha-MSH content of fractions was measured by RIA. Immunoreactive RP-HPLC fractions were further analyzed by electrospray mass spectrometry and the peptide sequence determined as SYSMEHFRWGKPV-NH2. In the pituitary gland of sea bream, des-, mono- and diacetyl alpha-MSH were identified. Then plasma alpha-MSH levels were measured in sea bream adapted to different backgrounds. Surprisingly, we found the highest plasma alpha-MSH levels in white-adapted as compared with black-adapted sea bream with intermediate values for gray-adapted fish. This observation is in contrast with results that have been obtained in eel, trout or terrestrial vertebrates. Next, des-, mono- and diacetyl alpha-MSH forms were measured in homogenates of the pituitary gland and in plasma of sea bream exposed to air, to confinement, or to different backgrounds. Monoacetyl alpha-MSH was the predominant form in all control and experimental groups. The lowest content of monoacetyl alpha-MSH relative to des- and diacetyl alpha-MSH was found in white-adapted fish. Levels of des- and diacetyl alpha-MSH forms were similar under all conditions. We observed that monoacetyl alpha-MSH is the most abundant isoform in the pituitary gland after background adaptation, confinement and air exposure, in sea bream. These data indicate that the physiologically most potent isoform of alpha-MSH may vary from species to species.</description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/joe.0.1660427</identifier><identifier>PMID: 10927632</identifier><identifier>CODEN: JOENAK</identifier><language>eng</language><publisher>Colchester: BioScientifica</publisher><subject>Acetylation ; Adaptation, Physiological ; alpha-MSH - isolation & purification ; alpha-MSH - metabolism ; Analysis of Variance ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Isomerism ; Male ; Mass Spectrometry - methods ; Pituitary Gland - metabolism ; Radioimmunoassay - methods ; Restraint, Physical ; Sea Bream - metabolism ; Sequence Analysis, Protein ; Stress, Psychological ; Vertebrates: endocrinology</subject><ispartof>Journal of endocrinology, 2000-08, Vol.166 (2), p.427-435</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b512t-8fe5d79bab1aa6b2208dc3cf249c7b4f089764a41782f8dd76477c82e2f0506b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1521715$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10927632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arends, RJ</creatorcontrib><creatorcontrib>Rotllant, J</creatorcontrib><creatorcontrib>Metz</creatorcontrib><creatorcontrib>Mancera, JM</creatorcontrib><creatorcontrib>Wendelaar Bonga, SE</creatorcontrib><creatorcontrib>Flik, G</creatorcontrib><creatorcontrib>Metz</creatorcontrib><title>alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description>MSH is a pituitary hormone derived by post-translational processing from POMC and involved in stress and background adaptation. N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH acetylation in the sea bream (Sparus aurata L.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. Pituitary homogenates were purified by reversed-phase HPLC (RP-HPLC). The alpha-MSH content of fractions was measured by RIA. Immunoreactive RP-HPLC fractions were further analyzed by electrospray mass spectrometry and the peptide sequence determined as SYSMEHFRWGKPV-NH2. In the pituitary gland of sea bream, des-, mono- and diacetyl alpha-MSH were identified. Then plasma alpha-MSH levels were measured in sea bream adapted to different backgrounds. Surprisingly, we found the highest plasma alpha-MSH levels in white-adapted as compared with black-adapted sea bream with intermediate values for gray-adapted fish. This observation is in contrast with results that have been obtained in eel, trout or terrestrial vertebrates. Next, des-, mono- and diacetyl alpha-MSH forms were measured in homogenates of the pituitary gland and in plasma of sea bream exposed to air, to confinement, or to different backgrounds. Monoacetyl alpha-MSH was the predominant form in all control and experimental groups. The lowest content of monoacetyl alpha-MSH relative to des- and diacetyl alpha-MSH was found in white-adapted fish. Levels of des- and diacetyl alpha-MSH forms were similar under all conditions. We observed that monoacetyl alpha-MSH is the most abundant isoform in the pituitary gland after background adaptation, confinement and air exposure, in sea bream. These data indicate that the physiologically most potent isoform of alpha-MSH may vary from species to species.</description><subject>Acetylation</subject><subject>Adaptation, Physiological</subject><subject>alpha-MSH - isolation & purification</subject><subject>alpha-MSH - metabolism</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Isomerism</subject><subject>Male</subject><subject>Mass Spectrometry - methods</subject><subject>Pituitary Gland - metabolism</subject><subject>Radioimmunoassay - methods</subject><subject>Restraint, Physical</subject><subject>Sea Bream - metabolism</subject><subject>Sequence Analysis, Protein</subject><subject>Stress, Psychological</subject><subject>Vertebrates: endocrinology</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS1ERZfCkSvyASGQmsV24jg5VhVQpEUcCudo7Ix3XZI42I6gv4M_3KRZCYRUTh7b37yx3yPkBWdbXir17sbjdilLVgj1iGx4oeqsrJh8TDaMCZExVctT8jTGG8a45Cp_Qk45q4Uqc7Ehv6EbD5B9vr6iYDDddpCcH6gbaDogHV2aXIJwS_cdDC319v44IlAdEHr65nqEMEUKU4AEdLd9u7QGjKMfItLkaeusxYBDohrM933w09DGc2r8YN2A_XKxKIMLFH-NPk4Bn5ETC13E58f1jHz78P7r5VW2-_Lx0-XFLtOSi5RVFmWrag2aA5RaCFa1JjdWFLVRurCsqlVZQMFVJWzVtvNGKVMJFJZJVur8jLxedcfgf0wYU9O7aLCbv4p-io0SMhc8VzOYraAJPsaAthmD62dbGs6aJYVmTqFZyvsUZv7lUXjSPbZ_0avtM_DqCEA00NkAg3HxDycFV1zOWLFiB7c__HQBG-18NG42zVln4MHx-dr2D_3_R98BNkmztg</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Arends, RJ</creator><creator>Rotllant, J</creator><creator>Metz</creator><creator>Mancera, JM</creator><creator>Wendelaar Bonga, SE</creator><creator>Flik, G</creator><general>BioScientifica</general><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure</title><author>Arends, RJ ; Rotllant, J ; Metz ; Mancera, JM ; Wendelaar Bonga, SE ; Flik, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b512t-8fe5d79bab1aa6b2208dc3cf249c7b4f089764a41782f8dd76477c82e2f0506b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acetylation</topic><topic>Adaptation, Physiological</topic><topic>alpha-MSH - isolation & purification</topic><topic>alpha-MSH - metabolism</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Isomerism</topic><topic>Male</topic><topic>Mass Spectrometry - methods</topic><topic>Pituitary Gland - metabolism</topic><topic>Radioimmunoassay - methods</topic><topic>Restraint, Physical</topic><topic>Sea Bream - metabolism</topic><topic>Sequence Analysis, Protein</topic><topic>Stress, Psychological</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arends, RJ</creatorcontrib><creatorcontrib>Rotllant, J</creatorcontrib><creatorcontrib>Metz</creatorcontrib><creatorcontrib>Mancera, JM</creatorcontrib><creatorcontrib>Wendelaar Bonga, SE</creatorcontrib><creatorcontrib>Flik, G</creatorcontrib><creatorcontrib>Metz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arends, RJ</au><au>Rotllant, J</au><au>Metz</au><au>Mancera, JM</au><au>Wendelaar Bonga, SE</au><au>Flik, G</au><aucorp>Metz</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>166</volume><issue>2</issue><spage>427</spage><epage>435</epage><pages>427-435</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><coden>JOENAK</coden><abstract>MSH is a pituitary hormone derived by post-translational processing from POMC and involved in stress and background adaptation. N-terminal acetylation of MSH to monoacetyl alpha-MSH or diacetyl alpha-MSH increases the bioactivity of the peptide. The aim of this study was to characterize alpha-MSH acetylation in the sea bream (Sparus aurata L.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. Pituitary homogenates were purified by reversed-phase HPLC (RP-HPLC). The alpha-MSH content of fractions was measured by RIA. Immunoreactive RP-HPLC fractions were further analyzed by electrospray mass spectrometry and the peptide sequence determined as SYSMEHFRWGKPV-NH2. In the pituitary gland of sea bream, des-, mono- and diacetyl alpha-MSH were identified. Then plasma alpha-MSH levels were measured in sea bream adapted to different backgrounds. Surprisingly, we found the highest plasma alpha-MSH levels in white-adapted as compared with black-adapted sea bream with intermediate values for gray-adapted fish. This observation is in contrast with results that have been obtained in eel, trout or terrestrial vertebrates. Next, des-, mono- and diacetyl alpha-MSH forms were measured in homogenates of the pituitary gland and in plasma of sea bream exposed to air, to confinement, or to different backgrounds. Monoacetyl alpha-MSH was the predominant form in all control and experimental groups. The lowest content of monoacetyl alpha-MSH relative to des- and diacetyl alpha-MSH was found in white-adapted fish. Levels of des- and diacetyl alpha-MSH forms were similar under all conditions. We observed that monoacetyl alpha-MSH is the most abundant isoform in the pituitary gland after background adaptation, confinement and air exposure, in sea bream. These data indicate that the physiologically most potent isoform of alpha-MSH may vary from species to species.</abstract><cop>Colchester</cop><pub>BioScientifica</pub><pmid>10927632</pmid><doi>10.1677/joe.0.1660427</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-0795 |
| ispartof | Journal of endocrinology, 2000-08, Vol.166 (2), p.427-435 |
| issn | 0022-0795 1479-6805 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72532137 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Acetylation Adaptation, Physiological alpha-MSH - isolation & purification alpha-MSH - metabolism Analysis of Variance Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Isomerism Male Mass Spectrometry - methods Pituitary Gland - metabolism Radioimmunoassay - methods Restraint, Physical Sea Bream - metabolism Sequence Analysis, Protein Stress, Psychological Vertebrates: endocrinology |
| title | alpha-MSH acetylation in the pituitary gland of the sea bream (Sparus aurata L.) in response to different backgrounds, confinement and air exposure |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T13%3A41%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=alpha-MSH%20acetylation%20in%20the%20pituitary%20gland%20of%20the%20sea%20bream%20(Sparus%20aurata%20L.)%20in%20response%20to%20different%20backgrounds,%20confinement%20and%20air%20exposure&rft.jtitle=Journal%20of%20endocrinology&rft.au=Arends,%20RJ&rft.aucorp=Metz&rft.date=2000-08-01&rft.volume=166&rft.issue=2&rft.spage=427&rft.epage=435&rft.pages=427-435&rft.issn=0022-0795&rft.eissn=1479-6805&rft.coden=JOENAK&rft_id=info:doi/10.1677/joe.0.1660427&rft_dat=%3Cproquest_cross%3E72532137%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72532137&rft_id=info:pmid/10927632&rfr_iscdi=true |