Application of monoliths for downstream processing of clotting factor IX
In this paper, the application of monolithic columns for downstream processing of different clotting factor IX concentrates is shown. Determination of basic chromatographic conditions as well as investigations on the regeneration of disk- and tube-shaped monolithic columns using human serum albumin...
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| Veröffentlicht in: | Journal of Chromatography A 2000-12, Vol.903 (1), p.21-32 |
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| container_title | Journal of Chromatography A |
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| creator | Branović, K Buchacher, A Barut, M Strancar, A Josić, Dj |
| description | In this paper, the application of monolithic columns for downstream processing of different clotting factor IX concentrates is shown. Determination of basic chromatographic conditions as well as investigations on the regeneration of disk- and tube-shaped monolithic columns using human serum albumin as a model protein, were performed. Separation of factor IX and vitronectin, a possible impurity in commercial factor IX concentrates was accomplished using disk-shaped monolithic columns. These same applications were also carried out with identical results on up-scaled tube-shaped monolithic columns. Since these media allow very fast separations, this method can be successfully applied not only to an in-process control of the purification of factor IX but also to other biopolymers from human plasma. Besides, the same application on the up-scaled tube-shaped monolithic column was successfully carried out. |
| doi_str_mv | 10.1016/S0021-9673(00)00899-2 |
| format | Article |
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Determination of basic chromatographic conditions as well as investigations on the regeneration of disk- and tube-shaped monolithic columns using human serum albumin as a model protein, were performed. Separation of factor IX and vitronectin, a possible impurity in commercial factor IX concentrates was accomplished using disk-shaped monolithic columns. These same applications were also carried out with identical results on up-scaled tube-shaped monolithic columns. Since these media allow very fast separations, this method can be successfully applied not only to an in-process control of the purification of factor IX but also to other biopolymers from human plasma. Besides, the same application on the up-scaled tube-shaped monolithic column was successfully carried out.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(00)00899-2</identifier><identifier>PMID: 11153943</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biopolymers ; Biotechnology ; Blood ; Chromatography, DEAE-Cellulose ; Coagulation factors ; Electrophoresis, Polyacrylamide Gel ; Factor IX ; Factor IX - chemistry ; Factor IX - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Humans ; Industrial applications and implications. 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Determination of basic chromatographic conditions as well as investigations on the regeneration of disk- and tube-shaped monolithic columns using human serum albumin as a model protein, were performed. Separation of factor IX and vitronectin, a possible impurity in commercial factor IX concentrates was accomplished using disk-shaped monolithic columns. These same applications were also carried out with identical results on up-scaled tube-shaped monolithic columns. Since these media allow very fast separations, this method can be successfully applied not only to an in-process control of the purification of factor IX but also to other biopolymers from human plasma. 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Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Production of active biomolecules</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Branović, K</creatorcontrib><creatorcontrib>Buchacher, A</creatorcontrib><creatorcontrib>Barut, M</creatorcontrib><creatorcontrib>Strancar, A</creatorcontrib><creatorcontrib>Josić, Dj</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Branović, K</au><au>Buchacher, A</au><au>Barut, M</au><au>Strancar, A</au><au>Josić, Dj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of monoliths for downstream processing of clotting factor IX</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2000-12</date><risdate>2000</risdate><volume>903</volume><issue>1</issue><spage>21</spage><epage>32</epage><pages>21-32</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>In this paper, the application of monolithic columns for downstream processing of different clotting factor IX concentrates is shown. 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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biopolymers Biotechnology Blood Chromatography, DEAE-Cellulose Coagulation factors Electrophoresis, Polyacrylamide Gel Factor IX Factor IX - chemistry Factor IX - isolation & purification Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Production of active biomolecules Reproducibility of Results |
| title | Application of monoliths for downstream processing of clotting factor IX |
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