Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa
To assess if testicular sperm cryopreservation is a valid alternative to repetition of testicular sperm retrieval techniques, results of a cryopreservation technique in pills have been retrospectively analyzed. Enough motile spermatozoa for ICSI were obtained in 172 from 190 (90.5%) frozen-thawed te...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2000-11, Vol.169 (1), p.15-19 |
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| creator | Gil-Salom, M Romero, J Rubio, C Ruiz, A Remohı́, J Pellicer, A |
| description | To assess if testicular sperm cryopreservation is a valid alternative to repetition of testicular sperm retrieval techniques, results of a cryopreservation technique in pills have been retrospectively analyzed. Enough motile spermatozoa for ICSI were obtained in 172 from 190 (90.5%) frozen-thawed testicular sperm samples. Overall, 249 couples underwent 390 ICSI cycles, 156 using fresh and 234 using cryopreserved testicular sperm. Mean two-pronuclear fertilization rates per cycle were not significantly different after ICSI with fresh (62.0%) or with cryopreserved (63.2%) spermatozoa. Mean embryo cleavage rate per cycle was higher in the fresh (90.6%) than in the cryopreserved (84.6%) group (
P=0.016). However, clinical pregnancy rates per cycle (28.2% with fresh vs 27.8% with cryopreserved), implantation rates (12.2% vs 13.1%) and ongoing pregnancy rates per cycle (22.4% vs 21.8%) were not significantly different. Cryopreservation of testicular spermatozoa is an effective technique that can be used both in obstructive and in non-obstructive azoospermia. |
| doi_str_mv | 10.1016/S0303-7207(00)00345-2 |
| format | Article |
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P=0.016). However, clinical pregnancy rates per cycle (28.2% with fresh vs 27.8% with cryopreserved), implantation rates (12.2% vs 13.1%) and ongoing pregnancy rates per cycle (22.4% vs 21.8%) were not significantly different. Cryopreservation of testicular spermatozoa is an effective technique that can be used both in obstructive and in non-obstructive azoospermia.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/S0303-7207(00)00345-2</identifier><identifier>PMID: 11155947</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adolescent ; Adult ; Azoospermia ; Cleavage Stage, Ovum ; Cryopreservation ; Cryopreservation - standards ; Female ; Fertilization ; Humans ; Intracytoplasmic sperm injection ; Male ; Male infertility ; Pregnancy ; Pregnancy Rate ; Retrospective Studies ; Semen Preservation - standards ; Sperm Injections, Intracytoplasmic - methods ; Sperm Injections, Intracytoplasmic - standards ; Spermatozoa ; Spermatozoa - cytology ; Spermatozoa - physiology ; Testis ; Testis - cytology</subject><ispartof>Molecular and cellular endocrinology, 2000-11, Vol.169 (1), p.15-19</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-42fe27745ca2a4fce78f29ee14039972bf71bd7ddd75a45878dd96c5e6ed6d3b3</citedby><cites>FETCH-LOGICAL-c361t-42fe27745ca2a4fce78f29ee14039972bf71bd7ddd75a45878dd96c5e6ed6d3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0303-7207(00)00345-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11155947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gil-Salom, M</creatorcontrib><creatorcontrib>Romero, J</creatorcontrib><creatorcontrib>Rubio, C</creatorcontrib><creatorcontrib>Ruiz, A</creatorcontrib><creatorcontrib>Remohı́, J</creatorcontrib><creatorcontrib>Pellicer, A</creatorcontrib><title>Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>To assess if testicular sperm cryopreservation is a valid alternative to repetition of testicular sperm retrieval techniques, results of a cryopreservation technique in pills have been retrospectively analyzed. Enough motile spermatozoa for ICSI were obtained in 172 from 190 (90.5%) frozen-thawed testicular sperm samples. Overall, 249 couples underwent 390 ICSI cycles, 156 using fresh and 234 using cryopreserved testicular sperm. Mean two-pronuclear fertilization rates per cycle were not significantly different after ICSI with fresh (62.0%) or with cryopreserved (63.2%) spermatozoa. Mean embryo cleavage rate per cycle was higher in the fresh (90.6%) than in the cryopreserved (84.6%) group (
P=0.016). However, clinical pregnancy rates per cycle (28.2% with fresh vs 27.8% with cryopreserved), implantation rates (12.2% vs 13.1%) and ongoing pregnancy rates per cycle (22.4% vs 21.8%) were not significantly different. Cryopreservation of testicular spermatozoa is an effective technique that can be used both in obstructive and in non-obstructive azoospermia.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Azoospermia</subject><subject>Cleavage Stage, Ovum</subject><subject>Cryopreservation</subject><subject>Cryopreservation - standards</subject><subject>Female</subject><subject>Fertilization</subject><subject>Humans</subject><subject>Intracytoplasmic sperm injection</subject><subject>Male</subject><subject>Male infertility</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Retrospective Studies</subject><subject>Semen Preservation - standards</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>Sperm Injections, Intracytoplasmic - standards</subject><subject>Spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><subject>Testis</subject><subject>Testis - cytology</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqXwCaCsECwCYyeOkxVCFU9VYgGsLdeeCFdJHOykqHw96UOwZDWbc-fOHEJOKVxRoNn1KySQxIKBuAC4BEhSHrM9Mqa5YHEOXOyT8S8yIkchLABAcJYfkhGllPMiFWPy_NR0XulV59pKhdrqKLTo68g2C9SddU30ZbuPSPuVaz0G9Es0UYehs7qvlN_SqnPfTh2Tg1JVAU92c0Le7-_epo_x7OXhaXo7i3WS0S5OWYlMiJRrxVRaahR5yQpEmkJSFILNS0HnRhhjBFcpz0VuTJFpjhmazCTzZELOt3tb7z774RRZ26CxqlSDrg9SMM4KTtMB5FtQexeCx1K23tbKryQFuZYoNxLl2pAEkBuJkg25s11BP6_R_KV21gbgZgvg8ObSopdBW2w0GusHa9I4-0_FD6mKg1w</recordid><startdate>20001127</startdate><enddate>20001127</enddate><creator>Gil-Salom, M</creator><creator>Romero, J</creator><creator>Rubio, C</creator><creator>Ruiz, A</creator><creator>Remohı́, J</creator><creator>Pellicer, A</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001127</creationdate><title>Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa</title><author>Gil-Salom, M ; Romero, J ; Rubio, C ; Ruiz, A ; Remohı́, J ; Pellicer, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-42fe27745ca2a4fce78f29ee14039972bf71bd7ddd75a45878dd96c5e6ed6d3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Azoospermia</topic><topic>Cleavage Stage, Ovum</topic><topic>Cryopreservation</topic><topic>Cryopreservation - standards</topic><topic>Female</topic><topic>Fertilization</topic><topic>Humans</topic><topic>Intracytoplasmic sperm injection</topic><topic>Male</topic><topic>Male infertility</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Retrospective Studies</topic><topic>Semen Preservation - standards</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>Sperm Injections, Intracytoplasmic - standards</topic><topic>Spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - physiology</topic><topic>Testis</topic><topic>Testis - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gil-Salom, M</creatorcontrib><creatorcontrib>Romero, J</creatorcontrib><creatorcontrib>Rubio, C</creatorcontrib><creatorcontrib>Ruiz, A</creatorcontrib><creatorcontrib>Remohı́, J</creatorcontrib><creatorcontrib>Pellicer, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gil-Salom, M</au><au>Romero, J</au><au>Rubio, C</au><au>Ruiz, A</au><au>Remohı́, J</au><au>Pellicer, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2000-11-27</date><risdate>2000</risdate><volume>169</volume><issue>1</issue><spage>15</spage><epage>19</epage><pages>15-19</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>To assess if testicular sperm cryopreservation is a valid alternative to repetition of testicular sperm retrieval techniques, results of a cryopreservation technique in pills have been retrospectively analyzed. Enough motile spermatozoa for ICSI were obtained in 172 from 190 (90.5%) frozen-thawed testicular sperm samples. Overall, 249 couples underwent 390 ICSI cycles, 156 using fresh and 234 using cryopreserved testicular sperm. Mean two-pronuclear fertilization rates per cycle were not significantly different after ICSI with fresh (62.0%) or with cryopreserved (63.2%) spermatozoa. Mean embryo cleavage rate per cycle was higher in the fresh (90.6%) than in the cryopreserved (84.6%) group (
P=0.016). However, clinical pregnancy rates per cycle (28.2% with fresh vs 27.8% with cryopreserved), implantation rates (12.2% vs 13.1%) and ongoing pregnancy rates per cycle (22.4% vs 21.8%) were not significantly different. Cryopreservation of testicular spermatozoa is an effective technique that can be used both in obstructive and in non-obstructive azoospermia.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>11155947</pmid><doi>10.1016/S0303-7207(00)00345-2</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adolescent Adult Azoospermia Cleavage Stage, Ovum Cryopreservation Cryopreservation - standards Female Fertilization Humans Intracytoplasmic sperm injection Male Male infertility Pregnancy Pregnancy Rate Retrospective Studies Semen Preservation - standards Sperm Injections, Intracytoplasmic - methods Sperm Injections, Intracytoplasmic - standards Spermatozoa Spermatozoa - cytology Spermatozoa - physiology Testis Testis - cytology |
| title | Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa |
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