DNA electrophoresis on a flat surface
We report a new approach for performing DNA electrophoresis. Using experimental studies and molecular dynamics simulations, we show that a perfectly flat silicon wafer, without any surface features, can be used to fractionate DNA in free solution. We determine that the ability of a flat surface to s...
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| Veröffentlicht in: | Physical review letters 2000-12, Vol.85 (26 Pt 1), p.5651-5654 |
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| container_end_page | 5654 |
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| container_issue | 26 Pt 1 |
| container_start_page | 5651 |
| container_title | Physical review letters |
| container_volume | 85 |
| creator | Pernodet, N Samuilov, V Shin, K Sokolov, J Rafailovich, M H Gersappe, D Chu, B |
| description | We report a new approach for performing DNA electrophoresis. Using experimental studies and molecular dynamics simulations, we show that a perfectly flat silicon wafer, without any surface features, can be used to fractionate DNA in free solution. We determine that the ability of a flat surface to separate DNA molecules results from the local friction between the surface and the adsorbed DNA segments. We control this friction by coating the Si surface with silane monolayer films and show that it is possible to systematically change the size range of DNA that can be separated. |
| doi_str_mv | 10.1103/PhysRevLett.85.5651 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0031-9007 |
| ispartof | Physical review letters, 2000-12, Vol.85 (26 Pt 1), p.5651-5654 |
| issn | 0031-9007 1079-7114 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72529404 |
| source | MEDLINE; American Physical Society Journals |
| subjects | Column chromatography Computer simulation DNA - chemistry DNA - isolation & purification Electrophoresis Entropy Fractionation Interfacial energy Microscopy, Atomic Force Molecular dynamics Molecules Silicon wafers Spectrometry, Fluorescence Surface Properties |
| title | DNA electrophoresis on a flat surface |
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