Expression and Catalytic Activity of Mouse Leukotriene B4 ω-Hydroxylase, CYP4F14

We have isolated a cDNA for a mouse leukotriene B4 ω-hydroxylase, CYP4F14. The cDNA encoded a protein with 524 amino acids, whose sequence similarity is 95% that of rat CYP4F1. The microsomes from yeast cells transfected with CYP4F14 expression vector showed 0.1 nmol P450/mg protein and catalyzed ω-...

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Veröffentlicht in:	Archives of biochemistry and biophysics 2000-11, Vol.383 (2), p.225-232
Hauptverfasser:	Kikuta, Yasushi, Kasyu, Hideaki, Kusunose, Emi, Kusunose, Masamichi
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Untranslated Regions 6-trans-leukotriene B4 Alternative Splicing Amino Acid Sequence Animals Arachidonic Acid - metabolism Base Sequence Blotting, Northern Brain - metabolism Catalysis cDNA cloning Clofibrate - pharmacology CYP4F14 Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism DNA, Complementary - metabolism Exons Hydroxyeicosatetraenoic Acids - metabolism Introns Laurates - metabolism leukotriene B4 Leukotriene B4 - metabolism Lipoxins Liver - metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Microsomes, Liver - enzymology Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Models, Genetic Molecular Sequence Data mouse liver Prostaglandins A - metabolism Rats Recombinant Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Homology, Amino Acid Substrate Specificity Tissue Distribution Transcription, Genetic Transfection ω-hydroxylase
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