Homologous functional expression of cryptic phaG from Pseudomonas oleovorans establishes the transacylase-mediated polyhydroxyalkanoate biosynthetic pathway

Various pseudomonads are capable of the synthesis of polyhydroxyalkanoate (PHA), composed of medium chain length (MCL) 3-hydroxy fatty acids (C6-C14), when grown on simple carbon sources such as, for example, gluconate or acetate. In Pseudomonas putida, the fatty acid de novo synthesis and PHA synth...

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Veröffentlicht in:	Applied microbiology and biotechnology 2000-11, Vol.54 (5), p.665-670
Hauptverfasser:	HOFFMANN, N, STEINBÜCHEL, A, REHM, B. H. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferases - chemistry Acyltransferases - genetics Acyltransferases - metabolism Amino Acid Sequence Amino acids Bacteria Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Blotting, Southern Carbon Carbon sources Cloning, Molecular Deoxyribonucleic acid DNA DNA, Bacterial - genetics Enzymes Fatty acids Fatty Acids - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Gene Transfer, Horizontal Genes, Bacterial Genetics Hybridization Hydroxy Acids - metabolism lac gene Microbiology Mission oriented research Molecular Sequence Data phaG gene phaGPo gene Plasmids Polyhydroxyalkanoates polyhydroxyalkanoic acid Pseudomonas - genetics Pseudomonas - metabolism Pseudomonas oleovorans Pseudomonas putida Pseudomonas putida - genetics Pseudomonas putida - metabolism Reintroduction Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA transacylase Transcription, Genetic
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