Cellular uptake and cytotoxicity of solid lipid nanospheres (SLN) incorporating doxorubicin or paclitaxel
Solid Lipid Nanospheres (SLN) are colloidal therapeutic systems proposed for several administration routes and obtained by dispersing warm microemulsions in cold water. SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytot...
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| Veröffentlicht in: | International journal of pharmaceutics 2000-12, Vol.210 (1), p.61-67 |
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| creator | Miglietta, Antonella Cavalli, Roberta Bocca, Claudia Gabriel, Ludovica Rosa Gasco, Maria |
| description | Solid Lipid Nanospheres (SLN) are colloidal therapeutic systems proposed for several administration routes and obtained by dispersing warm microemulsions in cold water. SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytotoxicity of doxorubicin and paclitaxel incorporated into SLN were investigated on two cell-lines, human promyelocytic leukemia (HL60) and human breast carcinoma (MCF-7). Cellular uptake of SLN was determined by incorporating 6-coumarin as fluorescent marker. The cellular uptake of fluorescent SLN was clearly evidenced by fluorescence microscopy. The cytotoxicity of doxorubicin incorporated in SLN was higher compared to the conventional doxorubicin solution, even at the lower concentrations. Paclitaxel in SLN was about 100-fold more effective than free paclitaxel on MCF-7 cells, while on HL60 cells a lower sensitivity was achieved with paclitaxel in SLN. Unloaded SLN had no cytotoxic effect on HL60 and MCF-7 cells. |
| doi_str_mv | 10.1016/S0378-5173(00)00562-7 |
| format | Article |
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SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytotoxicity of doxorubicin and paclitaxel incorporated into SLN were investigated on two cell-lines, human promyelocytic leukemia (HL60) and human breast carcinoma (MCF-7). Cellular uptake of SLN was determined by incorporating 6-coumarin as fluorescent marker. The cellular uptake of fluorescent SLN was clearly evidenced by fluorescence microscopy. The cytotoxicity of doxorubicin incorporated in SLN was higher compared to the conventional doxorubicin solution, even at the lower concentrations. Paclitaxel in SLN was about 100-fold more effective than free paclitaxel on MCF-7 cells, while on HL60 cells a lower sensitivity was achieved with paclitaxel in SLN. Unloaded SLN had no cytotoxic effect on HL60 and MCF-7 cells.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/S0378-5173(00)00562-7</identifier><identifier>PMID: 11163988</identifier><identifier>CODEN: IJPHDE</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Antibiotics, Antineoplastic - metabolism ; Antibiotics, Antineoplastic - pharmacology ; Antineoplastic agents ; Antineoplastic Agents, Phytogenic - metabolism ; Antineoplastic Agents, Phytogenic - pharmacology ; Biological and medical sciences ; Cell Survival - drug effects ; Cellular uptake ; Chemistry, Pharmaceutical ; Chemotherapy ; Cytotoxicity ; Doxorubicin - metabolism ; Doxorubicin - pharmacology ; Doxorubicin-loaded solid lipid nanospheres ; Fluorescent Dyes ; General pharmacology ; HL-60 Cells ; Humans ; Medical sciences ; Microscopy, Fluorescence ; Microspheres ; Paclitaxel - metabolism ; Paclitaxel - pharmacology ; Paclitaxel-loaded solid lipid nanospheres ; Pharmaceutical technology. 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SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytotoxicity of doxorubicin and paclitaxel incorporated into SLN were investigated on two cell-lines, human promyelocytic leukemia (HL60) and human breast carcinoma (MCF-7). Cellular uptake of SLN was determined by incorporating 6-coumarin as fluorescent marker. The cellular uptake of fluorescent SLN was clearly evidenced by fluorescence microscopy. The cytotoxicity of doxorubicin incorporated in SLN was higher compared to the conventional doxorubicin solution, even at the lower concentrations. Paclitaxel in SLN was about 100-fold more effective than free paclitaxel on MCF-7 cells, while on HL60 cells a lower sensitivity was achieved with paclitaxel in SLN. Unloaded SLN had no cytotoxic effect on HL60 and MCF-7 cells.</description><subject>Antibiotics, Antineoplastic - metabolism</subject><subject>Antibiotics, Antineoplastic - pharmacology</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents, Phytogenic - metabolism</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Cellular uptake</subject><subject>Chemistry, Pharmaceutical</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Doxorubicin - metabolism</subject><subject>Doxorubicin - pharmacology</subject><subject>Doxorubicin-loaded solid lipid nanospheres</subject><subject>Fluorescent Dyes</subject><subject>General pharmacology</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Microspheres</subject><subject>Paclitaxel - metabolism</subject><subject>Paclitaxel - pharmacology</subject><subject>Paclitaxel-loaded solid lipid nanospheres</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. 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Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miglietta, Antonella</creatorcontrib><creatorcontrib>Cavalli, Roberta</creatorcontrib><creatorcontrib>Bocca, Claudia</creatorcontrib><creatorcontrib>Gabriel, Ludovica</creatorcontrib><creatorcontrib>Rosa Gasco, Maria</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miglietta, Antonella</au><au>Cavalli, Roberta</au><au>Bocca, Claudia</au><au>Gabriel, Ludovica</au><au>Rosa Gasco, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular uptake and cytotoxicity of solid lipid nanospheres (SLN) incorporating doxorubicin or paclitaxel</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2000-12-04</date><risdate>2000</risdate><volume>210</volume><issue>1</issue><spage>61</spage><epage>67</epage><pages>61-67</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><coden>IJPHDE</coden><abstract>Solid Lipid Nanospheres (SLN) are colloidal therapeutic systems proposed for several administration routes and obtained by dispersing warm microemulsions in cold water. SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytotoxicity of doxorubicin and paclitaxel incorporated into SLN were investigated on two cell-lines, human promyelocytic leukemia (HL60) and human breast carcinoma (MCF-7). Cellular uptake of SLN was determined by incorporating 6-coumarin as fluorescent marker. The cellular uptake of fluorescent SLN was clearly evidenced by fluorescence microscopy. The cytotoxicity of doxorubicin incorporated in SLN was higher compared to the conventional doxorubicin solution, even at the lower concentrations. Paclitaxel in SLN was about 100-fold more effective than free paclitaxel on MCF-7 cells, while on HL60 cells a lower sensitivity was achieved with paclitaxel in SLN. Unloaded SLN had no cytotoxic effect on HL60 and MCF-7 cells.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11163988</pmid><doi>10.1016/S0378-5173(00)00562-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Antibiotics, Antineoplastic - metabolism Antibiotics, Antineoplastic - pharmacology Antineoplastic agents Antineoplastic Agents, Phytogenic - metabolism Antineoplastic Agents, Phytogenic - pharmacology Biological and medical sciences Cell Survival - drug effects Cellular uptake Chemistry, Pharmaceutical Chemotherapy Cytotoxicity Doxorubicin - metabolism Doxorubicin - pharmacology Doxorubicin-loaded solid lipid nanospheres Fluorescent Dyes General pharmacology HL-60 Cells Humans Medical sciences Microscopy, Fluorescence Microspheres Paclitaxel - metabolism Paclitaxel - pharmacology Paclitaxel-loaded solid lipid nanospheres Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Tumor Cells, Cultured |
| title | Cellular uptake and cytotoxicity of solid lipid nanospheres (SLN) incorporating doxorubicin or paclitaxel |
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