Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells
Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine a...
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| Veröffentlicht in: | Life sciences (1973) 2000-11, Vol.67 (24), p.2983-2989 |
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| container_end_page | 2989 |
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| container_issue | 24 |
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| container_title | Life sciences (1973) |
| container_volume | 67 |
| creator | Li, Zhiyuan Niwa, Yasuharu Sakamoto, Sadaichi Shono, Masayuki Chen, Xiu Nakaya, Yutaka |
| description | Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N
ω-Nitro-L-arginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca
2+ concentration. |
| doi_str_mv | 10.1016/S0024-3205(00)00880-8 |
| format | Article |
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ω-Nitro-L-arginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca
2+ concentration.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(00)00880-8</identifier><identifier>PMID: 11133010</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Aorta ; Arginine - pharmacology ; Ca 2+ influx ; Calcium - metabolism ; Cells, Cultured ; Drug Combinations ; Endothelial cell ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - enzymology ; Enzyme Induction ; Enzyme Inhibitors - pharmacology ; Ginsenosides ; Immunohistochemistry ; Nitric oxide production ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitric Oxide Synthase - biosynthesis ; Nitric Oxide Synthase Type II ; omega-N-Methylarginine - pharmacology ; Saponins - pharmacology ; Swine</subject><ispartof>Life sciences (1973), 2000-11, Vol.67 (24), p.2983-2989</ispartof><rights>2000 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-50745ff4649f5495d36baed51f15deb0f2e8d4a1723696e3fd8431b9f456a4e73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320500008808$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11133010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhiyuan</creatorcontrib><creatorcontrib>Niwa, Yasuharu</creatorcontrib><creatorcontrib>Sakamoto, Sadaichi</creatorcontrib><creatorcontrib>Shono, Masayuki</creatorcontrib><creatorcontrib>Chen, Xiu</creatorcontrib><creatorcontrib>Nakaya, Yutaka</creatorcontrib><title>Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N
ω-Nitro-L-arginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca
2+ concentration.</description><subject>Animals</subject><subject>Aorta</subject><subject>Arginine - pharmacology</subject><subject>Ca 2+ influx</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Drug Combinations</subject><subject>Endothelial cell</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - enzymology</subject><subject>Enzyme Induction</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Ginsenosides</subject><subject>Immunohistochemistry</subject><subject>Nitric oxide production</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Nitric Oxide Synthase Type II</subject><subject>omega-N-Methylarginine - pharmacology</subject><subject>Saponins - pharmacology</subject><subject>Swine</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMo7vr4CUpOoofqpEn6OImILxA8qOfQJhM30k3WpBX339t1Fz16Gmb45vURcsTgnAErLp4BcpHxHOQpwBlAVUFWbZEpq8o6g4KzbTL9RSZkL6V3AJCy5LtkwhjjHBhMSfvgzaB7FzwNlrpV4toOqXd9dJqGL2eQpqXvZ01C2i7pm_MJfUhjPY081UPXDxENXYSonUeK3oR-hp1rOqqx69IB2bFNl_BwE_fJ6-3Ny_V99vh093B99ZhpkZd9JqEU0lpRiNpKUUvDi7ZBI5ll0mALNsfKiIaVOS_qArk1leCsra2QRSOw5PvkZD13EcPHgKlXc5dWFzQew5BUmcucMQEjKNegjiGliFYtops3cakYqJVc9SNXrcwpAPUjV1Vj3_FmwdDO0fx1bWyOwOUawPHNT4dRJe3QazQuou6VCe6fFd9jvIpY</recordid><startdate>20001103</startdate><enddate>20001103</enddate><creator>Li, Zhiyuan</creator><creator>Niwa, Yasuharu</creator><creator>Sakamoto, Sadaichi</creator><creator>Shono, Masayuki</creator><creator>Chen, Xiu</creator><creator>Nakaya, Yutaka</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001103</creationdate><title>Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells</title><author>Li, Zhiyuan ; Niwa, Yasuharu ; Sakamoto, Sadaichi ; Shono, Masayuki ; Chen, Xiu ; Nakaya, Yutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-50745ff4649f5495d36baed51f15deb0f2e8d4a1723696e3fd8431b9f456a4e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Aorta</topic><topic>Arginine - pharmacology</topic><topic>Ca 2+ influx</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Drug Combinations</topic><topic>Endothelial cell</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - enzymology</topic><topic>Enzyme Induction</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Ginsenosides</topic><topic>Immunohistochemistry</topic><topic>Nitric oxide production</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Nitric Oxide Synthase Type II</topic><topic>omega-N-Methylarginine - pharmacology</topic><topic>Saponins - pharmacology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhiyuan</creatorcontrib><creatorcontrib>Niwa, Yasuharu</creatorcontrib><creatorcontrib>Sakamoto, Sadaichi</creatorcontrib><creatorcontrib>Shono, Masayuki</creatorcontrib><creatorcontrib>Chen, Xiu</creatorcontrib><creatorcontrib>Nakaya, Yutaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhiyuan</au><au>Niwa, Yasuharu</au><au>Sakamoto, Sadaichi</au><au>Shono, Masayuki</au><au>Chen, Xiu</au><au>Nakaya, Yutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2000-11-03</date><risdate>2000</risdate><volume>67</volume><issue>24</issue><spage>2983</spage><epage>2989</epage><pages>2983-2989</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N
ω-Nitro-L-arginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca
2+ concentration.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>11133010</pmid><doi>10.1016/S0024-3205(00)00880-8</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Aorta Arginine - pharmacology Ca 2+ influx Calcium - metabolism Cells, Cultured Drug Combinations Endothelial cell Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - enzymology Enzyme Induction Enzyme Inhibitors - pharmacology Ginsenosides Immunohistochemistry Nitric oxide production Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase - biosynthesis Nitric Oxide Synthase Type II omega-N-Methylarginine - pharmacology Saponins - pharmacology Swine |
| title | Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells |
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