Comparison of the Galleria baiting technique and a direct extraction method for recovering Steinernema (Nematoda: Rhabditida) infective-stage juveniles from soil
Forty soil samples from forests and other biotopes in Germany and the Czech Republic were studied for the presence of entomopathogenic nematodes using the Galleria bait method at the same time as a sieving-decanting method for direct extraction of infective-stage juveniles. Five Steinernema species...
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Veröffentlicht in: | Folia parasitologica 2000-01, Vol.47 (4), p.315-318 |
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description | Forty soil samples from forests and other biotopes in Germany and the Czech Republic were studied for the presence of entomopathogenic nematodes using the Galleria bait method at the same time as a sieving-decanting method for direct extraction of infective-stage juveniles. Five Steinernema species were recovered from the samples from Germany and four species from the samples from Czechia. All five species were recovered with both methods, but the baiting technique was generally less effective and mixtures of species were frequently undetected. The direct extraction method provided quantitative estimates of infective-stage juvenile density but no information on their infectivity or on morphological characters of adults, and nematode cultures could not be established. |
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Five Steinernema species were recovered from the samples from Germany and four species from the samples from Czechia. All five species were recovered with both methods, but the baiting technique was generally less effective and mixtures of species were frequently undetected. 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Five Steinernema species were recovered from the samples from Germany and four species from the samples from Czechia. All five species were recovered with both methods, but the baiting technique was generally less effective and mixtures of species were frequently undetected. The direct extraction method provided quantitative estimates of infective-stage juvenile density but no information on their infectivity or on morphological characters of adults, and nematode cultures could not be established.</description><subject>Animals</subject><subject>Czech Republic</subject><subject>Germany</subject><subject>Life Cycle Stages</subject><subject>Parasitology - methods</subject><subject>Rhabditida - growth & development</subject><subject>Rhabditida - isolation & purification</subject><subject>Soil - parasitology</subject><subject>Trees</subject><issn>0015-5683</issn><issn>1803-6465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkcFu1DAQhi1ERZfCjTPyCYFEFjuxnYQbWkFBqloJeo_G9rjrKrGD7V3B4_RNMXQlLp6Dv_-TZn5CXnG25UJw_sGt25YxtmVSPiEbPrCuUULJp2TDGJeNVEN3Tp7nfM-YkD1jz8g551zyUfYb8rCLywrJ5xhodLTskV7CPGPyQDX44sMdLWj2wf88IIVgKVDrE5pC8VdJYIqvyQXLPlrqYqL1Kx5rvOZ-FPQBU8AF6Nvr-pZo4SP9vgdtq9nCO-qDqyp_xCYXuEN6fzhi8DNm6lJcaI5-fkHOHMwZX57mBbn98vl297W5urn8tvt01ZiulaWBQYIC5doesNfctA6xRSEsWieMkkqP0upecO2Y7kbeCaVMP6Jwg9TD2F2QN4_aNcW6ai7T4rPBeYaA8ZCnvq0X47Kv4PtH0KSYc0I3rckvkH5PnE3_GpncOv1tZKqNVPz1yXvQC9r_8KmC7g8S4oqY</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Sturhan, D</creator><creator>Mrácek, Z</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>Comparison of the Galleria baiting technique and a direct extraction method for recovering Steinernema (Nematoda: Rhabditida) infective-stage juveniles from soil</title><author>Sturhan, D ; Mrácek, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-a85a6a6f27ae7b1c2fee2e44dedf4c656b95db741bf0b3913466c79e4f85b893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Czech Republic</topic><topic>Germany</topic><topic>Life Cycle Stages</topic><topic>Parasitology - methods</topic><topic>Rhabditida - growth & development</topic><topic>Rhabditida - isolation & purification</topic><topic>Soil - parasitology</topic><topic>Trees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sturhan, D</creatorcontrib><creatorcontrib>Mrácek, Z</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Folia parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sturhan, D</au><au>Mrácek, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the Galleria baiting technique and a direct extraction method for recovering Steinernema (Nematoda: Rhabditida) infective-stage juveniles from soil</atitle><jtitle>Folia parasitologica</jtitle><addtitle>Folia Parasitol (Praha)</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>47</volume><issue>4</issue><spage>315</spage><epage>318</epage><pages>315-318</pages><issn>0015-5683</issn><eissn>1803-6465</eissn><abstract>Forty soil samples from forests and other biotopes in Germany and the Czech Republic were studied for the presence of entomopathogenic nematodes using the Galleria bait method at the same time as a sieving-decanting method for direct extraction of infective-stage juveniles. 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subjects | Animals Czech Republic Germany Life Cycle Stages Parasitology - methods Rhabditida - growth & development Rhabditida - isolation & purification Soil - parasitology Trees |
title | Comparison of the Galleria baiting technique and a direct extraction method for recovering Steinernema (Nematoda: Rhabditida) infective-stage juveniles from soil |
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