Identification of two human glutaminase loci and tissue-specific expression of the two related genes
Glutaminolysis is initiated by either of two isoforms, K- and L-types, of the enzyme phosphate-activated glutaminase. The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results...
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| Veröffentlicht in: | Mammalian genome 2000-12, Vol.11 (12), p.1107-1110 |
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| creator | Aledo, J C Gómez-Fabre, P M Olalla, L Márquez, J |
| description | Glutaminolysis is initiated by either of two isoforms, K- and L-types, of the enzyme phosphate-activated glutaminase. The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results obtained from radiation hybrid mapping experiments assigned the K-glutaminase gene to human Chromosome (Chr) 2, and a second locus for L-glutaminase in Chr 12 was identified. Southern blot analysis with the L-cDNA probe showed hybridization to a single restriction fragment, while four to seven fragments were found to hybridize to the K-cDNA probe. The distribution of human glutaminase expression was also investigated: the L-cDNA probe detected a single band of 2.4 kb in liver, brain, and pancreas, whereas a single transcript of approximately 4.4 kb was detected in kidney, brain, heart, placenta, lung, and pancreas by using the K-cDNA probe. This work provides evidence that the human liver and kidney glutaminase isozymes are encoded by separate genes located on different chromosomes; furthermore, the expression pattern in human tissues revealed for both isoenzymes differs notably from the paradigm based upon the isoenzyme distribution in rats. |
| doi_str_mv | 10.1007/s003350010190 |
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The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results obtained from radiation hybrid mapping experiments assigned the K-glutaminase gene to human Chromosome (Chr) 2, and a second locus for L-glutaminase in Chr 12 was identified. Southern blot analysis with the L-cDNA probe showed hybridization to a single restriction fragment, while four to seven fragments were found to hybridize to the K-cDNA probe. The distribution of human glutaminase expression was also investigated: the L-cDNA probe detected a single band of 2.4 kb in liver, brain, and pancreas, whereas a single transcript of approximately 4.4 kb was detected in kidney, brain, heart, placenta, lung, and pancreas by using the K-cDNA probe. This work provides evidence that the human liver and kidney glutaminase isozymes are encoded by separate genes located on different chromosomes; furthermore, the expression pattern in human tissues revealed for both isoenzymes differs notably from the paradigm based upon the isoenzyme distribution in rats.</description><identifier>ISSN: 0938-8990</identifier><identifier>EISSN: 1432-1777</identifier><identifier>DOI: 10.1007/s003350010190</identifier><identifier>PMID: 11130979</identifier><language>eng</language><publisher>United States: Springer-Verlag</publisher><subject>Amino Acid Sequence ; Base Sequence ; chromosome 2 ; Chromosomes ; DNA Primers ; DNA Probes ; Gene Expression ; Genes ; Glutaminase - chemistry ; Glutaminase - genetics ; glutaminolysis ; Humans ; K-glutaminase ; L-glutaminase ; Molecular Sequence Data ; Proteins ; radiation hybrid mapping ; Sequence Homology, Amino Acid ; Tumor Cells, Cultured</subject><ispartof>Mammalian genome, 2000-12, Vol.11 (12), p.1107-1110</ispartof><rights>Springer-Verlag New York Inc. 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-8a5fda49978c7c08e5ad7a8206e7ef1dd0454395a44f739541fe76ac6fba8bb43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11130979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aledo, J C</creatorcontrib><creatorcontrib>Gómez-Fabre, P M</creatorcontrib><creatorcontrib>Olalla, L</creatorcontrib><creatorcontrib>Márquez, J</creatorcontrib><title>Identification of two human glutaminase loci and tissue-specific expression of the two related genes</title><title>Mammalian genome</title><addtitle>Mamm Genome</addtitle><description>Glutaminolysis is initiated by either of two isoforms, K- and L-types, of the enzyme phosphate-activated glutaminase. The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results obtained from radiation hybrid mapping experiments assigned the K-glutaminase gene to human Chromosome (Chr) 2, and a second locus for L-glutaminase in Chr 12 was identified. Southern blot analysis with the L-cDNA probe showed hybridization to a single restriction fragment, while four to seven fragments were found to hybridize to the K-cDNA probe. The distribution of human glutaminase expression was also investigated: the L-cDNA probe detected a single band of 2.4 kb in liver, brain, and pancreas, whereas a single transcript of approximately 4.4 kb was detected in kidney, brain, heart, placenta, lung, and pancreas by using the K-cDNA probe. 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The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results obtained from radiation hybrid mapping experiments assigned the K-glutaminase gene to human Chromosome (Chr) 2, and a second locus for L-glutaminase in Chr 12 was identified. Southern blot analysis with the L-cDNA probe showed hybridization to a single restriction fragment, while four to seven fragments were found to hybridize to the K-cDNA probe. The distribution of human glutaminase expression was also investigated: the L-cDNA probe detected a single band of 2.4 kb in liver, brain, and pancreas, whereas a single transcript of approximately 4.4 kb was detected in kidney, brain, heart, placenta, lung, and pancreas by using the K-cDNA probe. 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| subjects | Amino Acid Sequence Base Sequence chromosome 2 Chromosomes DNA Primers DNA Probes Gene Expression Genes Glutaminase - chemistry Glutaminase - genetics glutaminolysis Humans K-glutaminase L-glutaminase Molecular Sequence Data Proteins radiation hybrid mapping Sequence Homology, Amino Acid Tumor Cells, Cultured |
| title | Identification of two human glutaminase loci and tissue-specific expression of the two related genes |
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