Detection of small selenium-containing proteins in tissues of the rat
The important role of selenium in the mammalian organism has been manifested by the detection of several selenoenzymes, and there are still numerous selenium-containing proteins to be identified. After in vivo labeling of rats with [75Se]-selenite, gel electrophoretic separation of the proteins in t...
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| Veröffentlicht in: | Journal of trace elements in medicine and biology 2000-10, Vol.14 (3), p.179-183 |
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| container_title | Journal of trace elements in medicine and biology |
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| creator | Kyriakopoulos, A. Röthlein, D. Pfeifer, H. Bertelsmann, H. Kappler, S. Behne, D. |
| description | The important role of selenium in the mammalian organism has been manifested by the detection of several selenoenzymes, and there are still numerous selenium-containing proteins to be identified. After in vivo labeling of rats with [75Se]-selenite, gel electrophoretic separation of the proteins in tissue homogenates and autoradiography of the labeled bands, information on the selenium-containing proteins present in the different tissues was obtained. In the separation by SDS-PAGE and two-dimensional IEF/SDS-PAGE a large number of selenium-containing proteins or protein subunits with apparent molecular masses in the range from 116 to 8 kDa could be distinguished. This range was extended by applying a modified Tricine-SDS-PAGE, which allows the determination of smaller proteins. Using this method in the separation of the homogenates of the adrenal, brain, diaphragm, epididymis, heart, kidney, liver, lung, pituitary, prostate, skeletal muscle, spleen, thymus and thyroid, four additional selenium-containing proteins with molecular masses of approximately 7 kDa, 5kDa, 4 kDa and 3kDa were detected. The 5 kDa protein and the 7 kDa protein were identified as selenocysteine-containing selenoproteins. |
| doi_str_mv | 10.1016/S0946-672X(00)80008-5 |
| format | Article |
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After in vivo labeling of rats with [75Se]-selenite, gel electrophoretic separation of the proteins in tissue homogenates and autoradiography of the labeled bands, information on the selenium-containing proteins present in the different tissues was obtained. In the separation by SDS-PAGE and two-dimensional IEF/SDS-PAGE a large number of selenium-containing proteins or protein subunits with apparent molecular masses in the range from 116 to 8 kDa could be distinguished. This range was extended by applying a modified Tricine-SDS-PAGE, which allows the determination of smaller proteins. Using this method in the separation of the homogenates of the adrenal, brain, diaphragm, epididymis, heart, kidney, liver, lung, pituitary, prostate, skeletal muscle, spleen, thymus and thyroid, four additional selenium-containing proteins with molecular masses of approximately 7 kDa, 5kDa, 4 kDa and 3kDa were detected. The 5 kDa protein and the 7 kDa protein were identified as selenocysteine-containing selenoproteins.</description><identifier>ISSN: 0946-672X</identifier><identifier>EISSN: 1878-3252</identifier><identifier>DOI: 10.1016/S0946-672X(00)80008-5</identifier><identifier>PMID: 11130856</identifier><language>eng</language><publisher>Stuttgart: Elsevier GmbH</publisher><subject>2-D-electrophoresis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Electrophoresis, Gel, Two-Dimensional - methods ; Electrophoresis, Polyacrylamide Gel - methods ; Fundamental and applied biological sciences. Psychology ; Glycine - analogs & derivatives ; Isoelectric Focusing - methods ; Male ; Miscellaneous ; Molecular Weight ; Proteins ; Proteins - analysis ; Proteins - isolation & purification ; rat ; Rats ; Rats, Wistar ; Selenium ; Selenium - analysis ; Selenium Radioisotopes - analysis ; Selenoproteins ; Sodium Dodecyl Sulfate ; Tissue Distribution ; tricine-SDS-PAGE</subject><ispartof>Journal of trace elements in medicine and biology, 2000-10, Vol.14 (3), p.179-183</ispartof><rights>2000 Urban & Fischer Verlag</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-42805b05898af95417caa89995aa39c96791c7407b005bab830db01d78fd6a183</citedby><cites>FETCH-LOGICAL-c389t-42805b05898af95417caa89995aa39c96791c7407b005bab830db01d78fd6a183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0946672X00800085$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=814180$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11130856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kyriakopoulos, A.</creatorcontrib><creatorcontrib>Röthlein, D.</creatorcontrib><creatorcontrib>Pfeifer, H.</creatorcontrib><creatorcontrib>Bertelsmann, H.</creatorcontrib><creatorcontrib>Kappler, S.</creatorcontrib><creatorcontrib>Behne, D.</creatorcontrib><title>Detection of small selenium-containing proteins in tissues of the rat</title><title>Journal of trace elements in medicine and biology</title><addtitle>J Trace Elem Med Biol</addtitle><description>The important role of selenium in the mammalian organism has been manifested by the detection of several selenoenzymes, and there are still numerous selenium-containing proteins to be identified. After in vivo labeling of rats with [75Se]-selenite, gel electrophoretic separation of the proteins in tissue homogenates and autoradiography of the labeled bands, information on the selenium-containing proteins present in the different tissues was obtained. In the separation by SDS-PAGE and two-dimensional IEF/SDS-PAGE a large number of selenium-containing proteins or protein subunits with apparent molecular masses in the range from 116 to 8 kDa could be distinguished. This range was extended by applying a modified Tricine-SDS-PAGE, which allows the determination of smaller proteins. Using this method in the separation of the homogenates of the adrenal, brain, diaphragm, epididymis, heart, kidney, liver, lung, pituitary, prostate, skeletal muscle, spleen, thymus and thyroid, four additional selenium-containing proteins with molecular masses of approximately 7 kDa, 5kDa, 4 kDa and 3kDa were detected. The 5 kDa protein and the 7 kDa protein were identified as selenocysteine-containing selenoproteins.</description><subject>2-D-electrophoresis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Glycine - analogs & derivatives</topic><topic>Isoelectric Focusing - methods</topic><topic>Male</topic><topic>Miscellaneous</topic><topic>Molecular Weight</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteins - isolation & purification</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Selenium</topic><topic>Selenium - analysis</topic><topic>Selenium Radioisotopes - analysis</topic><topic>Selenoproteins</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Tissue Distribution</topic><topic>tricine-SDS-PAGE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kyriakopoulos, A.</creatorcontrib><creatorcontrib>Röthlein, D.</creatorcontrib><creatorcontrib>Pfeifer, H.</creatorcontrib><creatorcontrib>Bertelsmann, H.</creatorcontrib><creatorcontrib>Kappler, S.</creatorcontrib><creatorcontrib>Behne, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of trace elements in medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kyriakopoulos, A.</au><au>Röthlein, D.</au><au>Pfeifer, H.</au><au>Bertelsmann, H.</au><au>Kappler, S.</au><au>Behne, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of small selenium-containing proteins in tissues of the rat</atitle><jtitle>Journal of trace elements in medicine and biology</jtitle><addtitle>J Trace Elem Med Biol</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>14</volume><issue>3</issue><spage>179</spage><epage>183</epage><pages>179-183</pages><issn>0946-672X</issn><eissn>1878-3252</eissn><abstract>The important role of selenium in the mammalian organism has been manifested by the detection of several selenoenzymes, and there are still numerous selenium-containing proteins to be identified. After in vivo labeling of rats with [75Se]-selenite, gel electrophoretic separation of the proteins in tissue homogenates and autoradiography of the labeled bands, information on the selenium-containing proteins present in the different tissues was obtained. In the separation by SDS-PAGE and two-dimensional IEF/SDS-PAGE a large number of selenium-containing proteins or protein subunits with apparent molecular masses in the range from 116 to 8 kDa could be distinguished. This range was extended by applying a modified Tricine-SDS-PAGE, which allows the determination of smaller proteins. Using this method in the separation of the homogenates of the adrenal, brain, diaphragm, epididymis, heart, kidney, liver, lung, pituitary, prostate, skeletal muscle, spleen, thymus and thyroid, four additional selenium-containing proteins with molecular masses of approximately 7 kDa, 5kDa, 4 kDa and 3kDa were detected. 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| subjects | 2-D-electrophoresis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Glycine - analogs & derivatives Isoelectric Focusing - methods Male Miscellaneous Molecular Weight Proteins Proteins - analysis Proteins - isolation & purification rat Rats Rats, Wistar Selenium Selenium - analysis Selenium Radioisotopes - analysis Selenoproteins Sodium Dodecyl Sulfate Tissue Distribution tricine-SDS-PAGE |
| title | Detection of small selenium-containing proteins in tissues of the rat |
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