Lack of evidence for a role for the lipoxygenase pathway in increases in cytosolic calcium evoked by ADP and arachidonic acid in human platelets
We have investigated the possibility that metabolism of arachidonic acid by the lipoxygenase pathway contributes to ADP-evoked rises in [Ca 2+], in human platelets. 30μM BW A4C did not affect ADP-evoked Ca 2+ signals, but inhibited 12-lipoxygenase activity in platelet homogenates. Another lipoxygena...
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| Veröffentlicht in: | FEBS letters 1991-11, Vol.292 (1), p.196-200 |
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| description | We have investigated the possibility that metabolism of arachidonic acid by the lipoxygenase pathway contributes to ADP-evoked rises in [Ca
2+], in human platelets. 30μM BW A4C did not affect ADP-evoked Ca
2+ signals, but inhibited 12-lipoxygenase activity in platelet homogenates. Another lipoxygenase inhibitor, MK 866 was similary without effect on ADP-evoked Ca
2+ signals. ADP was found to liberate little arachidonic acid, and formation of the lipoxygenase product 12-HETE was not detectable. The rise in [Ca
2+]
i evoked by arachidonic acid was completely inhibited by the cyclooxygenase inhibitors aspirin or indomethacin. These results indicate that lipoxy genase products do not play an essential role in mediating rises in [Ca
2+]
i evoked by ADP, or by arachidonic acid. |
| doi_str_mv | 10.1016/0014-5793(91)80866-2 |
| format | Article |
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2+], in human platelets. 30μM BW A4C did not affect ADP-evoked Ca
2+ signals, but inhibited 12-lipoxygenase activity in platelet homogenates. Another lipoxygenase inhibitor, MK 866 was similary without effect on ADP-evoked Ca
2+ signals. ADP was found to liberate little arachidonic acid, and formation of the lipoxygenase product 12-HETE was not detectable. The rise in [Ca
2+]
i evoked by arachidonic acid was completely inhibited by the cyclooxygenase inhibitors aspirin or indomethacin. These results indicate that lipoxy genase products do not play an essential role in mediating rises in [Ca
2+]
i evoked by ADP, or by arachidonic acid.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)80866-2</identifier><identifier>PMID: 1959606</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>12- s-hydroxy-5,8- cis-10- trans- 14- cis-eicosotetraenoic acid ; 12-HETE ; 3[3-(4-chlorobenzyl)-3- t-butyl-thio-5-isopropylindol-2-yl]2,2-dimethyl-proanoic acid ; [Ca 2+] i ; Adenosine Diphosphate - metabolism ; ADP ; Affinity Labels ; Analytical, structural and metabolic biochemistry ; Arachidonic acid ; Arachidonic Acid - metabolism ; Biological and medical sciences ; Blood Platelets - drug effects ; Blood Platelets - enzymology ; Blood Platelets - metabolism ; BW A4C ; Calcium ; Calcium - metabolism ; Cyclooxygenase Inhibitors ; Cytosol - metabolism ; cytosolic calcium concentration ; Egtazic Acid - analogs & derivatives ; Egtazic Acid - chemistry ; Enzymes and enzyme inhibitors ; Fluorescent indicator ; Fundamental and applied biological sciences. Psychology ; Fura-2 - chemistry ; Humans ; Lipoxygenase - metabolism ; Lipoxygenase inhibitor ; Lipoxygenase Inhibitors ; MK 866 ; N-(4-benzyloxybenzyl)-acetohydroxamic acid ; NDGA ; nordihydroguaiaretic acid ; Oxidoreductases ; Platelet</subject><ispartof>FEBS letters, 1991-11, Vol.292 (1), p.196-200</ispartof><rights>1991 Federation of European Biochemical Societies</rights><rights>FEBS Letters 292 (1991) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4952-c6a9bfbbf3e7922b6dd1daaf3b9f2ad68b9b88a33ba1548c86b9ba5d3365ff13</citedby><cites>FETCH-LOGICAL-c4952-c6a9bfbbf3e7922b6dd1daaf3b9f2ad68b9b88a33ba1548c86b9ba5d3365ff13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(91)80866-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5011505$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1959606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vindlacheruvu, Raghu R.</creatorcontrib><creatorcontrib>Rink, Timothy J.</creatorcontrib><creatorcontrib>Sage, Stewart O.</creatorcontrib><title>Lack of evidence for a role for the lipoxygenase pathway in increases in cytosolic calcium evoked by ADP and arachidonic acid in human platelets</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We have investigated the possibility that metabolism of arachidonic acid by the lipoxygenase pathway contributes to ADP-evoked rises in [Ca
2+], in human platelets. 30μM BW A4C did not affect ADP-evoked Ca
2+ signals, but inhibited 12-lipoxygenase activity in platelet homogenates. Another lipoxygenase inhibitor, MK 866 was similary without effect on ADP-evoked Ca
2+ signals. ADP was found to liberate little arachidonic acid, and formation of the lipoxygenase product 12-HETE was not detectable. The rise in [Ca
2+]
i evoked by arachidonic acid was completely inhibited by the cyclooxygenase inhibitors aspirin or indomethacin. These results indicate that lipoxy genase products do not play an essential role in mediating rises in [Ca
2+]
i evoked by ADP, or by arachidonic acid.</description><subject>12- s-hydroxy-5,8- cis-10- trans- 14- cis-eicosotetraenoic acid</subject><subject>12-HETE</subject><subject>3[3-(4-chlorobenzyl)-3- t-butyl-thio-5-isopropylindol-2-yl]2,2-dimethyl-proanoic acid</subject><subject>[Ca 2+] i</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>ADP</subject><subject>Affinity Labels</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arachidonic acid</subject><subject>Arachidonic Acid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - enzymology</subject><subject>Blood Platelets - metabolism</subject><subject>BW A4C</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Cyclooxygenase Inhibitors</subject><subject>Cytosol - metabolism</subject><subject>cytosolic calcium concentration</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Egtazic Acid - chemistry</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fluorescent indicator</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fura-2 - chemistry</subject><subject>Humans</subject><subject>Lipoxygenase - metabolism</subject><subject>Lipoxygenase inhibitor</subject><subject>Lipoxygenase Inhibitors</subject><subject>MK 866</subject><subject>N-(4-benzyloxybenzyl)-acetohydroxamic acid</subject><subject>NDGA</subject><subject>nordihydroguaiaretic acid</subject><subject>Oxidoreductases</subject><subject>Platelet</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcFu1DAUjBCoLIU_AMkHhOghYCexE1-QSukWpJXg0Lv1Yj-zpkm82ElL_oJPxmlW5YaQLNlv3szYnpdlLxl9xygT7yllVc5rWb6V7KyhjRB58SjbsKYu87ISzeNs80B5mj2L8QdNdcPkSXbCJJeCik32ewf6hnhL8NYZHDQS6wMBEny3Hsc9ks4d_K_5Ow4QkRxg3N_BTNyQlg6YsLgUeh599J3TREOn3dQnS3-DhrQzOf_0jcBgCATQe2f8kFignVl0-6mHgRw6GLHDMT7PnljoIr447qfZ9fby-uJzvvt69eXifJfrSvIi1wJka9vWlljLomiFMcwA2LKVtgAjmla2TQNl2QLjVaMbkQDgpiwFt5aVp9mb1fYQ_M8J46h6FzV2HQzop6jqgtOa8yoRq5Wog48xoFWH4HoIs2JULXNQS8hqCVlJpu7noIoke3X0n9oezV_RGnzqvz72Iaa8bIBBu_hA45QxTnmibVfanetw_q-r1fbyY7E0Flyye3R5z4fVCFOmtw6Ditot8zYuoB6V8e7fH_oDfuW50g</recordid><startdate>19911104</startdate><enddate>19911104</enddate><creator>Vindlacheruvu, Raghu R.</creator><creator>Rink, Timothy J.</creator><creator>Sage, Stewart O.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911104</creationdate><title>Lack of evidence for a role for the lipoxygenase pathway in increases in cytosolic calcium evoked by ADP and arachidonic acid in human platelets</title><author>Vindlacheruvu, Raghu R. ; Rink, Timothy J. ; Sage, Stewart O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4952-c6a9bfbbf3e7922b6dd1daaf3b9f2ad68b9b88a33ba1548c86b9ba5d3365ff13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>12- s-hydroxy-5,8- cis-10- trans- 14- cis-eicosotetraenoic acid</topic><topic>12-HETE</topic><topic>3[3-(4-chlorobenzyl)-3- t-butyl-thio-5-isopropylindol-2-yl]2,2-dimethyl-proanoic acid</topic><topic>[Ca 2+] i</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>ADP</topic><topic>Affinity Labels</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arachidonic acid</topic><topic>Arachidonic Acid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - enzymology</topic><topic>Blood Platelets - metabolism</topic><topic>BW A4C</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Cyclooxygenase Inhibitors</topic><topic>Cytosol - metabolism</topic><topic>cytosolic calcium concentration</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Egtazic Acid - chemistry</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fluorescent indicator</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fura-2 - chemistry</topic><topic>Humans</topic><topic>Lipoxygenase - metabolism</topic><topic>Lipoxygenase inhibitor</topic><topic>Lipoxygenase Inhibitors</topic><topic>MK 866</topic><topic>N-(4-benzyloxybenzyl)-acetohydroxamic acid</topic><topic>NDGA</topic><topic>nordihydroguaiaretic acid</topic><topic>Oxidoreductases</topic><topic>Platelet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vindlacheruvu, Raghu R.</creatorcontrib><creatorcontrib>Rink, Timothy J.</creatorcontrib><creatorcontrib>Sage, Stewart O.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vindlacheruvu, Raghu R.</au><au>Rink, Timothy J.</au><au>Sage, Stewart O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of evidence for a role for the lipoxygenase pathway in increases in cytosolic calcium evoked by ADP and arachidonic acid in human platelets</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-11-04</date><risdate>1991</risdate><volume>292</volume><issue>1</issue><spage>196</spage><epage>200</epage><pages>196-200</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>We have investigated the possibility that metabolism of arachidonic acid by the lipoxygenase pathway contributes to ADP-evoked rises in [Ca
2+], in human platelets. 30μM BW A4C did not affect ADP-evoked Ca
2+ signals, but inhibited 12-lipoxygenase activity in platelet homogenates. Another lipoxygenase inhibitor, MK 866 was similary without effect on ADP-evoked Ca
2+ signals. ADP was found to liberate little arachidonic acid, and formation of the lipoxygenase product 12-HETE was not detectable. The rise in [Ca
2+]
i evoked by arachidonic acid was completely inhibited by the cyclooxygenase inhibitors aspirin or indomethacin. These results indicate that lipoxy genase products do not play an essential role in mediating rises in [Ca
2+]
i evoked by ADP, or by arachidonic acid.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1959606</pmid><doi>10.1016/0014-5793(91)80866-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1991-11, Vol.292 (1), p.196-200 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72507554 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 12- s-hydroxy-5,8- cis-10- trans- 14- cis-eicosotetraenoic acid 12-HETE 3[3-(4-chlorobenzyl)-3- t-butyl-thio-5-isopropylindol-2-yl]2,2-dimethyl-proanoic acid [Ca 2+] i Adenosine Diphosphate - metabolism ADP Affinity Labels Analytical, structural and metabolic biochemistry Arachidonic acid Arachidonic Acid - metabolism Biological and medical sciences Blood Platelets - drug effects Blood Platelets - enzymology Blood Platelets - metabolism BW A4C Calcium Calcium - metabolism Cyclooxygenase Inhibitors Cytosol - metabolism cytosolic calcium concentration Egtazic Acid - analogs & derivatives Egtazic Acid - chemistry Enzymes and enzyme inhibitors Fluorescent indicator Fundamental and applied biological sciences. Psychology Fura-2 - chemistry Humans Lipoxygenase - metabolism Lipoxygenase inhibitor Lipoxygenase Inhibitors MK 866 N-(4-benzyloxybenzyl)-acetohydroxamic acid NDGA nordihydroguaiaretic acid Oxidoreductases Platelet |
| title | Lack of evidence for a role for the lipoxygenase pathway in increases in cytosolic calcium evoked by ADP and arachidonic acid in human platelets |
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