The use of RNA/DNA ratio measurements to assess rifampicin-induced growth inhibition of Escherichia coli
Growth rates and cellular levels of RNA, DNA and protein were studied in Escherichia coli during and following exposure to rifampicin, and in the transition from stationary to exponential phase growth in drug-free medium. At rifampicin concentrations of up to twice the MIC, significant changes in gr...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 1991-09, Vol.28 (3), p.347-355 |
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| container_end_page | 355 |
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| container_issue | 3 |
| container_start_page | 347 |
| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 28 |
| creator | ELLARD, G. A |
| description | Growth rates and cellular levels of RNA, DNA and protein were studied in Escherichia coli during and following exposure to rifampicin, and in the transition from stationary to exponential phase growth in drug-free medium. At rifampicin concentrations of up to twice the MIC, significant changes in growth rates were only apparent after several generations. At higher rifampicin concentrations growth was terminated much more rapidly. In every case changes in growth rates were closely paralleled by corresponding changes in RNA/DNA ratios. These findings suggest the possibility of using RNA/DNA ratio measurements to monitor the drug-induced growth inhibition of other bacteria. |
| doi_str_mv | 10.1093/jac/28.3.347 |
| format | Article |
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These findings suggest the possibility of using RNA/DNA ratio measurements to monitor the drug-induced growth inhibition of other bacteria.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/28.3.347</identifier><identifier>PMID: 1720430</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacterial Proteins - analysis ; Bacterial Proteins - biosynthesis ; Biological and medical sciences ; DNA, Bacterial - analysis ; DNA, Bacterial - biosynthesis ; Dose-Response Relationship, Drug ; Escherichia coli - drug effects ; Escherichia coli - growth & development ; Medical sciences ; Pharmacology. 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A</creatorcontrib><title>The use of RNA/DNA ratio measurements to assess rifampicin-induced growth inhibition of Escherichia coli</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Growth rates and cellular levels of RNA, DNA and protein were studied in Escherichia coli during and following exposure to rifampicin, and in the transition from stationary to exponential phase growth in drug-free medium. At rifampicin concentrations of up to twice the MIC, significant changes in growth rates were only apparent after several generations. At higher rifampicin concentrations growth was terminated much more rapidly. In every case changes in growth rates were closely paralleled by corresponding changes in RNA/DNA ratios. These findings suggest the possibility of using RNA/DNA ratio measurements to monitor the drug-induced growth inhibition of other bacteria.</description><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - growth & development</subject><subject>Medical sciences</subject><subject>Pharmacology. 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Antiparasitic agents</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - growth & development</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Rifampin - pharmacology</topic><topic>RNA, Bacterial - analysis</topic><topic>RNA, Bacterial - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ELLARD, G. 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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of RNA/DNA ratio measurements to assess rifampicin-induced growth inhibition of Escherichia coli</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>28</volume><issue>3</issue><spage>347</spage><epage>355</epage><pages>347-355</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Growth rates and cellular levels of RNA, DNA and protein were studied in Escherichia coli during and following exposure to rifampicin, and in the transition from stationary to exponential phase growth in drug-free medium. At rifampicin concentrations of up to twice the MIC, significant changes in growth rates were only apparent after several generations. At higher rifampicin concentrations growth was terminated much more rapidly. In every case changes in growth rates were closely paralleled by corresponding changes in RNA/DNA ratios. These findings suggest the possibility of using RNA/DNA ratio measurements to monitor the drug-induced growth inhibition of other bacteria.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1720430</pmid><doi>10.1093/jac/28.3.347</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of antimicrobial chemotherapy, 1991-09, Vol.28 (3), p.347-355 |
| issn | 0305-7453 1460-2091 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Proteins - analysis Bacterial Proteins - biosynthesis Biological and medical sciences DNA, Bacterial - analysis DNA, Bacterial - biosynthesis Dose-Response Relationship, Drug Escherichia coli - drug effects Escherichia coli - growth & development Medical sciences Pharmacology. Drug treatments Rifampin - pharmacology RNA, Bacterial - analysis RNA, Bacterial - biosynthesis |
| title | The use of RNA/DNA ratio measurements to assess rifampicin-induced growth inhibition of Escherichia coli |
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