Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;16)
Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence o...
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| Veröffentlicht in: | Oncogene 2000-11, Vol.19 (50), p.5821-5825 |
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| creator | HOSAKA, Taisuke KANOE, Hiroshi NAKAMURA, Takashi TOGUCHIDA, Junya NAKAYAMA, Tomitaka MURAKAMI, Hiroshi YAMAMOTO, Hiroshi NAKAMATA, Takeharu TSUBOYAMA, Tadao OKA, Masanori KASAI, Masataka SASAKI, Masao S |
| description | Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our search for Translin binding motifs in sequences adjacent to breakpoints and investigated whether Translin binds to these sequences in vitro by mobility-shift assay. Computer-assisted search found sequences highly homologous (>70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas with the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding affinity of each motif showed considerable differences. The DNA-protein complex formation was inhibited by non-labeled competitor or anti-Translin antibody, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin binding motif may be one of the important determinants for the location of breakpoints in the TLS and CHOP genes in liposarcomas. |
| doi_str_mv | 10.1038/sj.onc.1203943 |
| format | Article |
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We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our search for Translin binding motifs in sequences adjacent to breakpoints and investigated whether Translin binds to these sequences in vitro by mobility-shift assay. Computer-assisted search found sequences highly homologous (>70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas with the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding affinity of each motif showed considerable differences. The DNA-protein complex formation was inhibited by non-labeled competitor or anti-Translin antibody, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin binding motif may be one of the important determinants for the location of breakpoints in the TLS and CHOP genes in liposarcomas.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1203943</identifier><identifier>PMID: 11126370</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Binding Sites ; Biological and medical sciences ; Bone surgery ; Breakpoints ; CCAAT-Enhancer-Binding Proteins - genetics ; Cell interactions, adhesion ; CHOP gene ; chromosome 12 ; chromosome 16 ; Chromosome Breakage ; Chromosome translocations ; Chromosomes ; Chromosomes, Human, Pair 12 ; Chromosomes, Human, Pair 16 ; Consensus Sequence ; DNA binding proteins ; DNA, Neoplasm - genetics ; DNA, Neoplasm - metabolism ; DNA-Binding Proteins - metabolism ; Electrophoretic mobility ; fibrosarcoma ; Fundamental and applied biological sciences. Psychology ; Fusion protein ; Genes ; Genetic aspects ; HeLa Cells ; Humans ; Leukemia ; liposarcoma ; Liposarcoma, Myxoid - genetics ; Liposarcoma, Myxoid - metabolism ; Molecular and cellular biology ; Oligonucleotides ; Oligonucleotides - genetics ; Oligonucleotides - metabolism ; Oncogene Proteins, Fusion - genetics ; Orthopedics ; Physiological aspects ; Proteins ; Risk factors ; RNA-Binding Protein FUS ; Sarcoma ; Substrate Specificity ; TLS gene ; Transcription Factor CHOP ; translin ; Translocation (Genetics) ; Translocation, Genetic ; TSN protein ; Tumors</subject><ispartof>Oncogene, 2000-11, Vol.19 (50), p.5821-5825</ispartof><rights>2001 INIST-CNRS</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 23, 2000</rights><rights>Macmillan Publishers Limited 2000.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-5948ad9cb7b828ac82e63cb4a7ffbfb0f31cf9d7ee1b93bc7d7cbdc653908b4a3</citedby><cites>FETCH-LOGICAL-c472t-5948ad9cb7b828ac82e63cb4a7ffbfb0f31cf9d7ee1b93bc7d7cbdc653908b4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=851329$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11126370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HOSAKA, Taisuke</creatorcontrib><creatorcontrib>KANOE, Hiroshi</creatorcontrib><creatorcontrib>NAKAMURA, Takashi</creatorcontrib><creatorcontrib>TOGUCHIDA, Junya</creatorcontrib><creatorcontrib>NAKAYAMA, Tomitaka</creatorcontrib><creatorcontrib>MURAKAMI, Hiroshi</creatorcontrib><creatorcontrib>YAMAMOTO, Hiroshi</creatorcontrib><creatorcontrib>NAKAMATA, Takeharu</creatorcontrib><creatorcontrib>TSUBOYAMA, Tadao</creatorcontrib><creatorcontrib>OKA, Masanori</creatorcontrib><creatorcontrib>KASAI, Masataka</creatorcontrib><creatorcontrib>SASAKI, Masao S</creatorcontrib><title>Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;16)</title><title>Oncogene</title><addtitle>Oncogene</addtitle><description>Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our search for Translin binding motifs in sequences adjacent to breakpoints and investigated whether Translin binds to these sequences in vitro by mobility-shift assay. Computer-assisted search found sequences highly homologous (>70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas with the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding affinity of each motif showed considerable differences. The DNA-protein complex formation was inhibited by non-labeled competitor or anti-Translin antibody, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin binding motif may be one of the important determinants for the location of breakpoints in the TLS and CHOP genes in liposarcomas.</description><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Bone surgery</subject><subject>Breakpoints</subject><subject>CCAAT-Enhancer-Binding Proteins - genetics</subject><subject>Cell interactions, adhesion</subject><subject>CHOP gene</subject><subject>chromosome 12</subject><subject>chromosome 16</subject><subject>Chromosome Breakage</subject><subject>Chromosome translocations</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, Pair 12</subject><subject>Chromosomes, Human, Pair 16</subject><subject>Consensus Sequence</subject><subject>DNA binding proteins</subject><subject>DNA, Neoplasm - genetics</subject><subject>DNA, Neoplasm - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoretic mobility</subject><subject>fibrosarcoma</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusion protein</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Leukemia</subject><subject>liposarcoma</subject><subject>Liposarcoma, Myxoid - genetics</subject><subject>Liposarcoma, Myxoid - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Oligonucleotides</subject><subject>Oligonucleotides - genetics</subject><subject>Oligonucleotides - metabolism</subject><subject>Oncogene Proteins, Fusion - genetics</subject><subject>Orthopedics</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Risk factors</subject><subject>RNA-Binding Protein FUS</subject><subject>Sarcoma</subject><subject>Substrate Specificity</subject><subject>TLS gene</subject><subject>Transcription Factor CHOP</subject><subject>translin</subject><subject>Translocation (Genetics)</subject><subject>Translocation, Genetic</subject><subject>TSN protein</subject><subject>Tumors</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks9rFDEUx4Modrt69ShBoehh1rwkM5ngqSxqhYUKrueQZJI240yyTmYRj_7nZutqRSqSQ-Dl8358874IPQGyAsLaV7lfpWhXQAmTnN1DC-Ciqepa8vtoQWRNKkkZPUGnOfeEECEJfYhOAIA2TJAF-r6ddMxDiNiE2GU8JzxfO5zdl72L1mWsu15bF-dfL2Zy-vMuhThnnPxNaLv5iHXs8Pri8gO-crFklXpD2KWsJ5tGnfHXMF_j-aZVsnoOKeL5BdDX0Lx8hB54PWT3-Hgv0ae3b7bri2pz-e79-nxTWS7oXBVFre6kNcK0tNW2pa5h1nAtvDfeEM_AetkJ58BIZqzohDWdbWomSVswtkRnP-vuplTE5VmNIVs3DDq6tM9K0JpADc1_QRCCcyJ4AZ__BfZpP8UiQtGGAwMOLRTq2T8pKhg0krDbUld6cCpEn8pn2UNfdU4J4aQ9bHiJVndQ5XRuDDZF50OJ35Vgp5Tz5LzaTWHU0zcFRB38o3Kvin_U0T8l4elx2L0ZXXeLHw3zh2adrR58WakN-TfX1sCoZD8AkvDLsA</recordid><startdate>20001123</startdate><enddate>20001123</enddate><creator>HOSAKA, Taisuke</creator><creator>KANOE, Hiroshi</creator><creator>NAKAMURA, Takashi</creator><creator>TOGUCHIDA, Junya</creator><creator>NAKAYAMA, Tomitaka</creator><creator>MURAKAMI, Hiroshi</creator><creator>YAMAMOTO, Hiroshi</creator><creator>NAKAMATA, Takeharu</creator><creator>TSUBOYAMA, Tadao</creator><creator>OKA, Masanori</creator><creator>KASAI, Masataka</creator><creator>SASAKI, Masao S</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20001123</creationdate><title>Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;16)</title><author>HOSAKA, Taisuke ; KANOE, Hiroshi ; NAKAMURA, Takashi ; TOGUCHIDA, Junya ; NAKAYAMA, Tomitaka ; MURAKAMI, Hiroshi ; YAMAMOTO, Hiroshi ; NAKAMATA, Takeharu ; TSUBOYAMA, Tadao ; OKA, Masanori ; KASAI, Masataka ; SASAKI, Masao S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-5948ad9cb7b828ac82e63cb4a7ffbfb0f31cf9d7ee1b93bc7d7cbdc653908b4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Bone surgery</topic><topic>Breakpoints</topic><topic>CCAAT-Enhancer-Binding Proteins - genetics</topic><topic>Cell interactions, adhesion</topic><topic>CHOP gene</topic><topic>chromosome 12</topic><topic>chromosome 16</topic><topic>Chromosome Breakage</topic><topic>Chromosome translocations</topic><topic>Chromosomes</topic><topic>Chromosomes, Human, Pair 12</topic><topic>Chromosomes, Human, Pair 16</topic><topic>Consensus Sequence</topic><topic>DNA binding proteins</topic><topic>DNA, Neoplasm - genetics</topic><topic>DNA, Neoplasm - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoretic mobility</topic><topic>fibrosarcoma</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusion protein</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Leukemia</topic><topic>liposarcoma</topic><topic>Liposarcoma, Myxoid - genetics</topic><topic>Liposarcoma, Myxoid - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Oligonucleotides</topic><topic>Oligonucleotides - genetics</topic><topic>Oligonucleotides - metabolism</topic><topic>Oncogene Proteins, Fusion - genetics</topic><topic>Orthopedics</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Risk factors</topic><topic>RNA-Binding Protein FUS</topic><topic>Sarcoma</topic><topic>Substrate Specificity</topic><topic>TLS gene</topic><topic>Transcription Factor CHOP</topic><topic>translin</topic><topic>Translocation (Genetics)</topic><topic>Translocation, Genetic</topic><topic>TSN protein</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOSAKA, Taisuke</creatorcontrib><creatorcontrib>KANOE, Hiroshi</creatorcontrib><creatorcontrib>NAKAMURA, Takashi</creatorcontrib><creatorcontrib>TOGUCHIDA, Junya</creatorcontrib><creatorcontrib>NAKAYAMA, Tomitaka</creatorcontrib><creatorcontrib>MURAKAMI, Hiroshi</creatorcontrib><creatorcontrib>YAMAMOTO, Hiroshi</creatorcontrib><creatorcontrib>NAKAMATA, Takeharu</creatorcontrib><creatorcontrib>TSUBOYAMA, Tadao</creatorcontrib><creatorcontrib>OKA, Masanori</creatorcontrib><creatorcontrib>KASAI, Masataka</creatorcontrib><creatorcontrib>SASAKI, Masao S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOSAKA, Taisuke</au><au>KANOE, Hiroshi</au><au>NAKAMURA, Takashi</au><au>TOGUCHIDA, Junya</au><au>NAKAYAMA, Tomitaka</au><au>MURAKAMI, Hiroshi</au><au>YAMAMOTO, Hiroshi</au><au>NAKAMATA, Takeharu</au><au>TSUBOYAMA, Tadao</au><au>OKA, Masanori</au><au>KASAI, Masataka</au><au>SASAKI, Masao S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;16)</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>2000-11-23</date><risdate>2000</risdate><volume>19</volume><issue>50</issue><spage>5821</spage><epage>5825</epage><pages>5821-5825</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><coden>ONCNES</coden><abstract>Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our search for Translin binding motifs in sequences adjacent to breakpoints and investigated whether Translin binds to these sequences in vitro by mobility-shift assay. Computer-assisted search found sequences highly homologous (>70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas with the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding affinity of each motif showed considerable differences. The DNA-protein complex formation was inhibited by non-labeled competitor or anti-Translin antibody, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin binding motif may be one of the important determinants for the location of breakpoints in the TLS and CHOP genes in liposarcomas.</abstract><cop>Basingstoke</cop><pub>Nature Publishing</pub><pmid>11126370</pmid><doi>10.1038/sj.onc.1203943</doi><tpages>5</tpages></addata></record> |
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| ispartof | Oncogene, 2000-11, Vol.19 (50), p.5821-5825 |
| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | MEDLINE; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Binding Sites Biological and medical sciences Bone surgery Breakpoints CCAAT-Enhancer-Binding Proteins - genetics Cell interactions, adhesion CHOP gene chromosome 12 chromosome 16 Chromosome Breakage Chromosome translocations Chromosomes Chromosomes, Human, Pair 12 Chromosomes, Human, Pair 16 Consensus Sequence DNA binding proteins DNA, Neoplasm - genetics DNA, Neoplasm - metabolism DNA-Binding Proteins - metabolism Electrophoretic mobility fibrosarcoma Fundamental and applied biological sciences. Psychology Fusion protein Genes Genetic aspects HeLa Cells Humans Leukemia liposarcoma Liposarcoma, Myxoid - genetics Liposarcoma, Myxoid - metabolism Molecular and cellular biology Oligonucleotides Oligonucleotides - genetics Oligonucleotides - metabolism Oncogene Proteins, Fusion - genetics Orthopedics Physiological aspects Proteins Risk factors RNA-Binding Protein FUS Sarcoma Substrate Specificity TLS gene Transcription Factor CHOP translin Translocation (Genetics) Translocation, Genetic TSN protein Tumors |
| title | Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;16) |
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