Regulation of cellular water and ionic content in lungs of fetal and adult rats
Slices of lungs from late-fetal (1 day pre-partum) and adult rats lost K+ and gained Na+, Cl-, water and Ca2+ during pre-incubation at 1 degrees C. These changes were reversed upon restoration to 37 degrees C. The recovery of composition at 37 degrees C was completely dependent on cell respiration i...
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| Veröffentlicht in: | Experimental physiology 1991-09, Vol.76 (5), p.745-763 |
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| container_title | Experimental physiology |
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| creator | Mariani, MF Thomas, L Russo, MA van Rossum, GD |
| description | Slices of lungs from late-fetal (1 day pre-partum) and adult rats lost K+ and gained Na+, Cl-, water and Ca2+ during pre-incubation
at 1 degrees C. These changes were reversed upon restoration to 37 degrees C. The recovery of composition at 37 degrees C
was completely dependent on cell respiration in adult slices; by contrast, glycolysis could support partial recovery in the
fetal slices. Ouabain completely inhibited K+ reaccumulation at both ages but inhibited net extrusion of water by no more
than 50%. Replacement of medium Cl- with NO3- prevented the extrusion of water in the presence of ouabain in adult but not
fetal slices. Transmission electron microscopy of type II epithelial cells in slices of both ages showed that ouabain induced
the formation of many cytoplasmic vesicles, apparently derived from the Golgi apparatus. Regulation of cell ionic and water
content is thus generally similar in late-fetal and adult lung tissue, but there are differences in the source of ATP and
in some features of ouabain-resistant volume regulation. |
| doi_str_mv | 10.1113/expphysiol.1991.sp003541 |
| format | Article |
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at 1 degrees C. These changes were reversed upon restoration to 37 degrees C. The recovery of composition at 37 degrees C
was completely dependent on cell respiration in adult slices; by contrast, glycolysis could support partial recovery in the
fetal slices. Ouabain completely inhibited K+ reaccumulation at both ages but inhibited net extrusion of water by no more
than 50%. Replacement of medium Cl- with NO3- prevented the extrusion of water in the presence of ouabain in adult but not
fetal slices. Transmission electron microscopy of type II epithelial cells in slices of both ages showed that ouabain induced
the formation of many cytoplasmic vesicles, apparently derived from the Golgi apparatus. Regulation of cell ionic and water
content is thus generally similar in late-fetal and adult lung tissue, but there are differences in the source of ATP and
in some features of ouabain-resistant volume regulation.</description><identifier>ISSN: 0958-0670</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1113/expphysiol.1991.sp003541</identifier><identifier>PMID: 1742014</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Biological Transport, Active ; Body Water - metabolism ; Energy Metabolism ; Female ; Lung - embryology ; Lung - metabolism ; Lung - ultrastructure ; Male ; Ouabain - pharmacology ; Potassium - metabolism ; Pregnancy ; Rats ; Rats, Inbred Strains</subject><ispartof>Experimental physiology, 1991-09, Vol.76 (5), p.745-763</ispartof><rights>1991 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4515-fa02ce7a9289c4d161e01061b53b1f19def7fca7c984100d81102fd2dcbbdd6a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1113%2Fexpphysiol.1991.sp003541$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1113%2Fexpphysiol.1991.sp003541$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1742014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mariani, MF</creatorcontrib><creatorcontrib>Thomas, L</creatorcontrib><creatorcontrib>Russo, MA</creatorcontrib><creatorcontrib>van Rossum, GD</creatorcontrib><title>Regulation of cellular water and ionic content in lungs of fetal and adult rats</title><title>Experimental physiology</title><addtitle>Exp Physiol</addtitle><description>Slices of lungs from late-fetal (1 day pre-partum) and adult rats lost K+ and gained Na+, Cl-, water and Ca2+ during pre-incubation
at 1 degrees C. These changes were reversed upon restoration to 37 degrees C. The recovery of composition at 37 degrees C
was completely dependent on cell respiration in adult slices; by contrast, glycolysis could support partial recovery in the
fetal slices. Ouabain completely inhibited K+ reaccumulation at both ages but inhibited net extrusion of water by no more
than 50%. Replacement of medium Cl- with NO3- prevented the extrusion of water in the presence of ouabain in adult but not
fetal slices. Transmission electron microscopy of type II epithelial cells in slices of both ages showed that ouabain induced
the formation of many cytoplasmic vesicles, apparently derived from the Golgi apparatus. Regulation of cell ionic and water
content is thus generally similar in late-fetal and adult lung tissue, but there are differences in the source of ATP and
in some features of ouabain-resistant volume regulation.</description><subject>Animals</subject><subject>Biological Transport, Active</subject><subject>Body Water - metabolism</subject><subject>Energy Metabolism</subject><subject>Female</subject><subject>Lung - embryology</subject><subject>Lung - metabolism</subject><subject>Lung - ultrastructure</subject><subject>Male</subject><subject>Ouabain - pharmacology</subject><subject>Potassium - metabolism</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0958-0670</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9v2yAYh9G0qs3afYRJnHZzxmuDMccq6ppKlTpNnbQbwvxJmIjtAVaab1-77pTrTgh-z-8FHoQwkDUAVN_syzDsT8n3YQ1CwDoNhFSMwge0AlqLglL2-yNaEcGagtScXKFPKf0hBCrS0Et0CZyWBOgKPf20uzGo7PsO9w5rG8K0jfioso1YdQZPiddY9122Xca-w2HsdmmGnc0qvDHKjCHjqHK6QRdOhWQ_v6_X6Nf3u-fNtnh8un_Y3D4WmjJghVOk1JYrUTZCUwM1WAKkhpZVLTgQxjrutOJaNBQIMQ0AKZ0pjW5bY2pVXaOvy9wh9n9Hm7I8-DS_XnW2H5PkJRWCiXoCmwXUsU8pWieH6A8qniQQObuUZ5dydin_uZyqX97vGNuDNefiIm_KN0t-9MGe_nuuvPuxnQ94zThl55_s_W5_9NHKpZV67W0-SV5LJmfwFdZfl8o</recordid><startdate>19910901</startdate><enddate>19910901</enddate><creator>Mariani, MF</creator><creator>Thomas, L</creator><creator>Russo, MA</creator><creator>van Rossum, GD</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910901</creationdate><title>Regulation of cellular water and ionic content in lungs of fetal and adult rats</title><author>Mariani, MF ; Thomas, L ; Russo, MA ; van Rossum, GD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4515-fa02ce7a9289c4d161e01061b53b1f19def7fca7c984100d81102fd2dcbbdd6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological Transport, Active</topic><topic>Body Water - metabolism</topic><topic>Energy Metabolism</topic><topic>Female</topic><topic>Lung - embryology</topic><topic>Lung - metabolism</topic><topic>Lung - ultrastructure</topic><topic>Male</topic><topic>Ouabain - pharmacology</topic><topic>Potassium - metabolism</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mariani, MF</creatorcontrib><creatorcontrib>Thomas, L</creatorcontrib><creatorcontrib>Russo, MA</creatorcontrib><creatorcontrib>van Rossum, GD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mariani, MF</au><au>Thomas, L</au><au>Russo, MA</au><au>van Rossum, GD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of cellular water and ionic content in lungs of fetal and adult rats</atitle><jtitle>Experimental physiology</jtitle><addtitle>Exp Physiol</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>76</volume><issue>5</issue><spage>745</spage><epage>763</epage><pages>745-763</pages><issn>0958-0670</issn><eissn>1469-445X</eissn><abstract>Slices of lungs from late-fetal (1 day pre-partum) and adult rats lost K+ and gained Na+, Cl-, water and Ca2+ during pre-incubation
at 1 degrees C. These changes were reversed upon restoration to 37 degrees C. The recovery of composition at 37 degrees C
was completely dependent on cell respiration in adult slices; by contrast, glycolysis could support partial recovery in the
fetal slices. Ouabain completely inhibited K+ reaccumulation at both ages but inhibited net extrusion of water by no more
than 50%. Replacement of medium Cl- with NO3- prevented the extrusion of water in the presence of ouabain in adult but not
fetal slices. Transmission electron microscopy of type II epithelial cells in slices of both ages showed that ouabain induced
the formation of many cytoplasmic vesicles, apparently derived from the Golgi apparatus. Regulation of cell ionic and water
content is thus generally similar in late-fetal and adult lung tissue, but there are differences in the source of ATP and
in some features of ouabain-resistant volume regulation.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>1742014</pmid><doi>10.1113/expphysiol.1991.sp003541</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0958-0670 |
| ispartof | Experimental physiology, 1991-09, Vol.76 (5), p.745-763 |
| issn | 0958-0670 1469-445X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72499596 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Biological Transport, Active Body Water - metabolism Energy Metabolism Female Lung - embryology Lung - metabolism Lung - ultrastructure Male Ouabain - pharmacology Potassium - metabolism Pregnancy Rats Rats, Inbred Strains |
| title | Regulation of cellular water and ionic content in lungs of fetal and adult rats |
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