Isolation and nucleotide sequence of the Thiobacillus ferrooxidans genes for the small and large subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase
The genes encoding for the large (rbcL) and small (rbcS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisCO) were cloned from the obligate autotroph Thiobacillus ferrooxidans, a bacterium involved in the bioleaching of minerals. Nucleotide sequence analysis of the cloned DNA showed that the...
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| Veröffentlicht in: | FEBS letters 1991-11, Vol.292 (1-2), p.85-89 |
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| creator | PULGAR, V GAETE, L ALLENDE, J ORELLANA, O JORDANA, X JEDLICKI, E |
| description | The genes encoding for the large (rbcL) and small (rbcS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisCO) were cloned from the obligate autotroph Thiobacillus ferrooxidans, a bacterium involved in the bioleaching of minerals. Nucleotide sequence analysis of the cloned DNA showed that the two coding regions are separated by a 30-bp intergenic region, the smallest described for the RuBisCO genes. The rbcL and rbcS genes encode polypeptides of 473 and 118 amino acids, respectively. Comparison of the nucleotide and amino acid sequences with those of the genes for rbcL and rbcS found in other species demonstrated that the T. ferrooxidans genes have the closest degree of identity with those of Chromatium vinosum and of Alvinoconcha hessleri endosymbiont. Both T. ferrooxidans enzyme subunits contain all the conserved amino acids that are known to participate in the catalytic process or in holoenzyme assembly. |
| doi_str_mv | 10.1016/0014-5793(91)80840-Y |
| format | Article |
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Nucleotide sequence analysis of the cloned DNA showed that the two coding regions are separated by a 30-bp intergenic region, the smallest described for the RuBisCO genes. The rbcL and rbcS genes encode polypeptides of 473 and 118 amino acids, respectively. Comparison of the nucleotide and amino acid sequences with those of the genes for rbcL and rbcS found in other species demonstrated that the T. ferrooxidans genes have the closest degree of identity with those of Chromatium vinosum and of Alvinoconcha hessleri endosymbiont. Both T. ferrooxidans enzyme subunits contain all the conserved amino acids that are known to participate in the catalytic process or in holoenzyme assembly.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)80840-Y</identifier><identifier>PMID: 1959634</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Chromosomes, Bacterial ; Cloning, Molecular ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; genes ; Genes, Bacterial ; Genes. Genome ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequence ; predictions ; Restriction Mapping ; ribulose-bisphosphate carboxylase ; Ribulose-Bisphosphate Carboxylase - genetics ; Sequence Homology, Nucleic Acid ; subunits ; Thiobacillus - genetics ; Thiobacillus ferrooxidans</subject><ispartof>FEBS letters, 1991-11, Vol.292 (1-2), p.85-89</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5008821$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1959634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PULGAR, V</creatorcontrib><creatorcontrib>GAETE, L</creatorcontrib><creatorcontrib>ALLENDE, J</creatorcontrib><creatorcontrib>ORELLANA, O</creatorcontrib><creatorcontrib>JORDANA, X</creatorcontrib><creatorcontrib>JEDLICKI, E</creatorcontrib><title>Isolation and nucleotide sequence of the Thiobacillus ferrooxidans genes for the small and large subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The genes encoding for the large (rbcL) and small (rbcS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisCO) were cloned from the obligate autotroph Thiobacillus ferrooxidans, a bacterium involved in the bioleaching of minerals. Nucleotide sequence analysis of the cloned DNA showed that the two coding regions are separated by a 30-bp intergenic region, the smallest described for the RuBisCO genes. The rbcL and rbcS genes encode polypeptides of 473 and 118 amino acids, respectively. Comparison of the nucleotide and amino acid sequences with those of the genes for rbcL and rbcS found in other species demonstrated that the T. ferrooxidans genes have the closest degree of identity with those of Chromatium vinosum and of Alvinoconcha hessleri endosymbiont. Both T. ferrooxidans enzyme subunits contain all the conserved amino acids that are known to participate in the catalytic process or in holoenzyme assembly.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Bacterial</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>predictions</subject><subject>Restriction Mapping</subject><subject>ribulose-bisphosphate carboxylase</subject><subject>Ribulose-Bisphosphate Carboxylase - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>subunits</subject><subject>Thiobacillus - genetics</subject><subject>Thiobacillus ferrooxidans</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rVTEQhoMo9Vr9BwpZiCh4bL5OTrIsxY9CwU1duLpMcia9kdzkmpwD7X_xx5rWS7cuwuTNPPPAhJDXnH3ijOszxrgaxsnK95Z_MMwoNvx8QjbcTHKQSpunZPOIPCcvWvvFejbcnpATbkerpdqQP5etJFhiyRTyTPPqE5Ylzkgb_l4xe6Ql0GWH9HoXiwMfU1obDVhrKbdxhtzoDWbsT6U-cG0PKT3IEtSbnle35ri0e0-Nbk2lIeUfx8HFdtiVfmBB6qG6cnuXoOFZr13Zby_JswCp4atjPSU_vny-vvg2XH3_enlxfjUchNbLAKAMBG5mkMwJIwWfAtNcKTV6EWwQGIzxfkbttedm9FYK52ftZqn7kJCn5N0_76GWvnRbtvvYPKYEGcvatpNQ1go5_RfkWjAr7b3xzRFc3R7n7aHGPdS77fHfe__tsQ_NQwoVso_tERsZM0Zw-RejCJY2</recordid><startdate>19911104</startdate><enddate>19911104</enddate><creator>PULGAR, V</creator><creator>GAETE, L</creator><creator>ALLENDE, J</creator><creator>ORELLANA, O</creator><creator>JORDANA, X</creator><creator>JEDLICKI, E</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19911104</creationdate><title>Isolation and nucleotide sequence of the Thiobacillus ferrooxidans genes for the small and large subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase</title><author>PULGAR, V ; GAETE, L ; ALLENDE, J ; ORELLANA, O ; JORDANA, X ; JEDLICKI, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-aa48af18da30b283217f0614445c2f9f2ef88ccde6c6c185c932bcd6bd368da23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Bacterial</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Genes. Genome</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>predictions</topic><topic>Restriction Mapping</topic><topic>ribulose-bisphosphate carboxylase</topic><topic>Ribulose-Bisphosphate Carboxylase - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>subunits</topic><topic>Thiobacillus - genetics</topic><topic>Thiobacillus ferrooxidans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PULGAR, V</creatorcontrib><creatorcontrib>GAETE, L</creatorcontrib><creatorcontrib>ALLENDE, J</creatorcontrib><creatorcontrib>ORELLANA, O</creatorcontrib><creatorcontrib>JORDANA, X</creatorcontrib><creatorcontrib>JEDLICKI, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PULGAR, V</au><au>GAETE, L</au><au>ALLENDE, J</au><au>ORELLANA, O</au><au>JORDANA, X</au><au>JEDLICKI, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and nucleotide sequence of the Thiobacillus ferrooxidans genes for the small and large subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-11-04</date><risdate>1991</risdate><volume>292</volume><issue>1-2</issue><spage>85</spage><epage>89</epage><pages>85-89</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The genes encoding for the large (rbcL) and small (rbcS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisCO) were cloned from the obligate autotroph Thiobacillus ferrooxidans, a bacterium involved in the bioleaching of minerals. Nucleotide sequence analysis of the cloned DNA showed that the two coding regions are separated by a 30-bp intergenic region, the smallest described for the RuBisCO genes. The rbcL and rbcS genes encode polypeptides of 473 and 118 amino acids, respectively. Comparison of the nucleotide and amino acid sequences with those of the genes for rbcL and rbcS found in other species demonstrated that the T. ferrooxidans genes have the closest degree of identity with those of Chromatium vinosum and of Alvinoconcha hessleri endosymbiont. Both T. ferrooxidans enzyme subunits contain all the conserved amino acids that are known to participate in the catalytic process or in holoenzyme assembly.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>1959634</pmid><doi>10.1016/0014-5793(91)80840-Y</doi><tpages>5</tpages></addata></record> |
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| ispartof | FEBS letters, 1991-11, Vol.292 (1-2), p.85-89 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Chromosomes, Bacterial Cloning, Molecular DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology genes Genes, Bacterial Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequence predictions Restriction Mapping ribulose-bisphosphate carboxylase Ribulose-Bisphosphate Carboxylase - genetics Sequence Homology, Nucleic Acid subunits Thiobacillus - genetics Thiobacillus ferrooxidans |
| title | Isolation and nucleotide sequence of the Thiobacillus ferrooxidans genes for the small and large subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase |
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