Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations
The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children...
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| Veröffentlicht in: | Circulation research 1991-12, Vol.69 (6), p.1601-1607 |
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| container_title | Circulation research |
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| creator | Shi, Qinwei Danilczyk, Ursula Wang, Jinxia See, Yew P Williams, William G Trusler, George A Beaulieu, R Rose, Vera Jackowski, George |
| description | The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children with different forms of congenital heart disease. Perimembranous ventricular septal defects and tetralogy of Fallot were associated with the presence of ventricular myosin light chains in 50% of patients. The presence of ventricular myosin light chains in these atria did not correlate with pressure or volume overload. Analysis of myosin heavy chain isotype in the same biopsies by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis indicated that there was no detectable expression of ventricular myosin heavy chain (β-subunit), suggesting that the genes for the myosin heavy chains and light chains are not expressed coordinately. |
| doi_str_mv | 10.1161/01.RES.69.6.1601 |
| format | Article |
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Ventricular myosin light chains were present in 27% of biopsies from 91 children with different forms of congenital heart disease. Perimembranous ventricular septal defects and tetralogy of Fallot were associated with the presence of ventricular myosin light chains in 50% of patients. The presence of ventricular myosin light chains in these atria did not correlate with pressure or volume overload. Analysis of myosin heavy chain isotype in the same biopsies by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis indicated that there was no detectable expression of ventricular myosin heavy chain (β-subunit), suggesting that the genes for the myosin heavy chains and light chains are not expressed coordinately.</description><identifier>ISSN: 0009-7330</identifier><identifier>EISSN: 1524-4571</identifier><identifier>DOI: 10.1161/01.RES.69.6.1601</identifier><identifier>PMID: 1954679</identifier><identifier>CODEN: CIRUAL</identifier><language>eng</language><publisher>Hagerstown, MD: American Heart Association, Inc</publisher><subject>Biological and medical sciences ; Blood Pressure ; Blotting, Western ; Cardiology. Vascular system ; Congenital heart diseases. Malformations of the aorta, pulmonary vessels and vena cava ; Electrophoresis, Gel, Two-Dimensional ; Heart ; Heart Atria - chemistry ; Heart Defects, Congenital - physiopathology ; Heart Ventricles - chemistry ; Humans ; Medical sciences ; Myosins - chemistry ; Peptide Mapping</subject><ispartof>Circulation research, 1991-12, Vol.69 (6), p.1601-1607</ispartof><rights>1991 American Heart Association, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4478-b7103233b4b21dbae0f5fe5273fae58dc4aefd1faf812e6b37977730db4bbcc93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3674,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5595322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1954679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Qinwei</creatorcontrib><creatorcontrib>Danilczyk, Ursula</creatorcontrib><creatorcontrib>Wang, Jinxia</creatorcontrib><creatorcontrib>See, Yew P</creatorcontrib><creatorcontrib>Williams, William G</creatorcontrib><creatorcontrib>Trusler, George A</creatorcontrib><creatorcontrib>Beaulieu, R</creatorcontrib><creatorcontrib>Rose, Vera</creatorcontrib><creatorcontrib>Jackowski, George</creatorcontrib><title>Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations</title><title>Circulation research</title><addtitle>Circ Res</addtitle><description>The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children with different forms of congenital heart disease. Perimembranous ventricular septal defects and tetralogy of Fallot were associated with the presence of ventricular myosin light chains in 50% of patients. The presence of ventricular myosin light chains in these atria did not correlate with pressure or volume overload. Analysis of myosin heavy chain isotype in the same biopsies by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis indicated that there was no detectable expression of ventricular myosin heavy chain (β-subunit), suggesting that the genes for the myosin heavy chains and light chains are not expressed coordinately.</description><subject>Biological and medical sciences</subject><subject>Blood Pressure</subject><subject>Blotting, Western</subject><subject>Cardiology. Vascular system</subject><subject>Congenital heart diseases. Malformations of the aorta, pulmonary vessels and vena cava</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Heart</subject><subject>Heart Atria - chemistry</subject><subject>Heart Defects, Congenital - physiopathology</subject><subject>Heart Ventricles - chemistry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Myosins - chemistry</subject><subject>Peptide Mapping</subject><issn>0009-7330</issn><issn>1524-4571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1v1DAQxS0EKkvhzgXJB8QtweOPeH2sVluK1AqJlvZoOcmYGLzJYidq-9_j1a7gYNmj-b03mmdC3gOrARr4zKD-vr2tG1M3NTQMXpAVKC4rqTS8JCvGmKm0EOw1eZPzL8ZACm7OyBkYJRttVsRvn_YJcw7TSCdP73GcU-iW6BK9eZ5yGOnt0i5jmDMt73lAelEAd2A3Q4h9wpE-hHmgm2n8iYVzkV6hSzO9cdFPaefmYp3fklfexYzvTvc5-XG5vdtcVdffvnzdXFxXnZR6XbUamOBCtLLl0LcOmVceFdfCO1TrvpMOfQ_e-TVwbFqhjdZasL4I2q4z4px8Ovru0_RnwTzbXcgdxuhGnJZsNZemDOIFZEewS1POCb3dp7Bz6dkCs4doLQNborWNsY09RFskH07eS7vD_r_gmGXpfzz1Xe7K8smNXcj_MKWMEvwwWR6xxynOmPLvuDxisgO6OA-2_BgTDHgFxgDwUlXlwFr8BdAeklg</recordid><startdate>199112</startdate><enddate>199112</enddate><creator>Shi, Qinwei</creator><creator>Danilczyk, Ursula</creator><creator>Wang, Jinxia</creator><creator>See, Yew P</creator><creator>Williams, William G</creator><creator>Trusler, George A</creator><creator>Beaulieu, R</creator><creator>Rose, Vera</creator><creator>Jackowski, George</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199112</creationdate><title>Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations</title><author>Shi, Qinwei ; Danilczyk, Ursula ; Wang, Jinxia ; See, Yew P ; Williams, William G ; Trusler, George A ; Beaulieu, R ; Rose, Vera ; Jackowski, George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4478-b7103233b4b21dbae0f5fe5273fae58dc4aefd1faf812e6b37977730db4bbcc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>Blood Pressure</topic><topic>Blotting, Western</topic><topic>Cardiology. Vascular system</topic><topic>Congenital heart diseases. Malformations of the aorta, pulmonary vessels and vena cava</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Heart</topic><topic>Heart Atria - chemistry</topic><topic>Heart Defects, Congenital - physiopathology</topic><topic>Heart Ventricles - chemistry</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Myosins - chemistry</topic><topic>Peptide Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Qinwei</creatorcontrib><creatorcontrib>Danilczyk, Ursula</creatorcontrib><creatorcontrib>Wang, Jinxia</creatorcontrib><creatorcontrib>See, Yew P</creatorcontrib><creatorcontrib>Williams, William G</creatorcontrib><creatorcontrib>Trusler, George A</creatorcontrib><creatorcontrib>Beaulieu, R</creatorcontrib><creatorcontrib>Rose, Vera</creatorcontrib><creatorcontrib>Jackowski, George</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Qinwei</au><au>Danilczyk, Ursula</au><au>Wang, Jinxia</au><au>See, Yew P</au><au>Williams, William G</au><au>Trusler, George A</au><au>Beaulieu, R</au><au>Rose, Vera</au><au>Jackowski, George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations</atitle><jtitle>Circulation research</jtitle><addtitle>Circ Res</addtitle><date>1991-12</date><risdate>1991</risdate><volume>69</volume><issue>6</issue><spage>1601</spage><epage>1607</epage><pages>1601-1607</pages><issn>0009-7330</issn><eissn>1524-4571</eissn><coden>CIRUAL</coden><abstract>The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children with different forms of congenital heart disease. Perimembranous ventricular septal defects and tetralogy of Fallot were associated with the presence of ventricular myosin light chains in 50% of patients. The presence of ventricular myosin light chains in these atria did not correlate with pressure or volume overload. Analysis of myosin heavy chain isotype in the same biopsies by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis indicated that there was no detectable expression of ventricular myosin heavy chain (β-subunit), suggesting that the genes for the myosin heavy chains and light chains are not expressed coordinately.</abstract><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>1954679</pmid><doi>10.1161/01.RES.69.6.1601</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-7330 |
| ispartof | Circulation research, 1991-12, Vol.69 (6), p.1601-1607 |
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| language | eng |
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| source | MEDLINE; American Heart Association Journals; EZB-FREE-00999 freely available EZB journals; Journals@Ovid Complete |
| subjects | Biological and medical sciences Blood Pressure Blotting, Western Cardiology. Vascular system Congenital heart diseases. Malformations of the aorta, pulmonary vessels and vena cava Electrophoresis, Gel, Two-Dimensional Heart Heart Atria - chemistry Heart Defects, Congenital - physiopathology Heart Ventricles - chemistry Humans Medical sciences Myosins - chemistry Peptide Mapping |
| title | Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations |
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