Endogenous activity of cyclic nucleotide-dependent protein kinase in plasma membranes isolated from Strongylocentrotus purpuratus sea urchin sperm

Activity of cyclic nucleotide-dependent protein kinase was investigated in flagellar plasma membranes of sea urchin sperm ( S. purpuratus). Membranes incubated with [γ- 32P]ATP showed in the presence of 1 μM cAMP an increased phosphorylation in multiple polypeptides. Half maximal response was seen a...

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Veröffentlicht in:	Biochemical and biophysical research communications 1991-11, Vol.180 (3), p.1436-1445
Hauptverfasser:	Garcia-Soto, Jesus, Araiza, Luz M., Barrios, Miriam, Darszon, Alberto, Luna-Arias, Juan P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Fractionation Cell Membrane - enzymology Cyclic AMP - pharmacology Cyclic GMP - pharmacology Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Kinetics Male Membrane Proteins - isolation & purification Membrane Proteins - metabolism Molecular Weight Phosphorylation Protein Kinases - isolation & purification Protein Kinases - metabolism Sea Urchins Spermatozoa - metabolism Transferases
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