Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin

Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were...

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Veröffentlicht in:Biochemical and biophysical research communications 1991-10, Vol.180 (2), p.481-488
Hauptverfasser: Johnson, Gibbes R., Saeki, Toshiaki, Auersperg, Nelly, Gordon, Alfred W., Shoyab, Mohammed, Salomon, David S., Stromberg, Kurt
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container_end_page 488
container_issue 2
container_start_page 481
container_title Biochemical and biophysical research communications
container_volume 180
creator Johnson, Gibbes R.
Saeki, Toshiaki
Auersperg, Nelly
Gordon, Alfred W.
Shoyab, Mohammed
Salomon, David S.
Stromberg, Kurt
description Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. These results suggest that AR may play a growth regulatory role in the nucleus of cells and this role may be different in normal and malignant epithelial cells.
doi_str_mv 10.1016/S0006-291X(05)81090-3
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Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. 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Psychology</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - pharmacology</subject><subject>Growth Substances - analysis</subject><subject>Growth Substances - pharmacology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Molecular and cellular biology</subject><subject>Ovarian Neoplasms</subject><subject>Ovary</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUd1qFTEYDGKpp0cfoZALEXuxmuwm2V1vRIraQlHwB7wL3ybftpHdZE12i_VZfFhzfjjFq-YmgZnJDDOEnHL2ijOuXn9ljKmibPmPl0yeNZy1rKgekdX2UXImHpPVgfKEnKT0kzHOhWqPyTFvZVXzdkX-fsE0BZ-QzoGCtxR_TxFTcsHT0FMYpxsX8XoZnKfdHQ23EB14aiAa58MIW40PcYThAKYl9mCQ4uTmGxxchgwOQ3pDPy1mQIh0CAYG9wfmvQ16G67RhyX95_iUHPUwJHy2v9fk-4f3384viqvPHy_P310VRjRqLrDrsSsVACtZLWtRC2HqVijWcKytai0DgbbnsqlqabmQhres6lBCbcpKQbUmL3b_TjH8WjDNenRpExk85ky6LkWbm5MPErniSlT5rIncEU0MKUXs9RTdCPFOc6Y38-ntfHqzjWZSb-fTG93p3mDpRrT3qt1eGX--xyHlCvsI3rh0oEnelEI1mfZ2R8Pc2q3DqJNx6A3aXK2ZtQ3ugSD_ADDKum8</recordid><startdate>19911031</startdate><enddate>19911031</enddate><creator>Johnson, Gibbes R.</creator><creator>Saeki, Toshiaki</creator><creator>Auersperg, Nelly</creator><creator>Gordon, Alfred W.</creator><creator>Shoyab, Mohammed</creator><creator>Salomon, David S.</creator><creator>Stromberg, Kurt</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911031</creationdate><title>Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin</title><author>Johnson, Gibbes R. ; Saeki, Toshiaki ; Auersperg, Nelly ; Gordon, Alfred W. ; Shoyab, Mohammed ; Salomon, David S. ; Stromberg, Kurt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-ebfeb26aa0207574744c7946081e7d69d0a4edf158375d145c1903be5a7c236a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amphiregulin</topic><topic>Biological and medical sciences</topic><topic>carcinoma</topic><topic>Cell Division - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell physiology</topic><topic>EGF Family of Proteins</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. 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ispartof Biochemical and biophysical research communications, 1991-10, Vol.180 (2), p.481-488
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subjects Amphiregulin
Biological and medical sciences
carcinoma
Cell Division - drug effects
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Cell physiology
EGF Family of Proteins
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Epithelium - drug effects
Epithelium - metabolism
Female
Fundamental and applied biological sciences. Psychology
Glycoproteins - analysis
Glycoproteins - pharmacology
Growth Substances - analysis
Growth Substances - pharmacology
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins
Molecular and cellular biology
Ovarian Neoplasms
Ovary
Responses to growth factors, tumor promotors, other factors
title Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin
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