Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin
Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were...
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Veröffentlicht in: | Biochemical and biophysical research communications 1991-10, Vol.180 (2), p.481-488 |
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creator | Johnson, Gibbes R. Saeki, Toshiaki Auersperg, Nelly Gordon, Alfred W. Shoyab, Mohammed Salomon, David S. Stromberg, Kurt |
description | Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. These results suggest that AR may play a growth regulatory role in the nucleus of cells and this role may be different in normal and malignant epithelial cells. |
doi_str_mv | 10.1016/S0006-291X(05)81090-3 |
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Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. These results suggest that AR may play a growth regulatory role in the nucleus of cells and this role may be different in normal and malignant epithelial cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(05)81090-3</identifier><identifier>PMID: 1953719</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amphiregulin ; Biological and medical sciences ; carcinoma ; Cell Division - drug effects ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cell physiology ; EGF Family of Proteins ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells ; Epithelium - drug effects ; Epithelium - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - analysis ; Glycoproteins - pharmacology ; Growth Substances - analysis ; Growth Substances - pharmacology ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; Molecular and cellular biology ; Ovarian Neoplasms ; Ovary ; Responses to growth factors, tumor promotors, other factors</subject><ispartof>Biochemical and biophysical research communications, 1991-10, Vol.180 (2), p.481-488</ispartof><rights>1991 Academic Press, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-ebfeb26aa0207574744c7946081e7d69d0a4edf158375d145c1903be5a7c236a3</citedby><cites>FETCH-LOGICAL-c486t-ebfeb26aa0207574744c7946081e7d69d0a4edf158375d145c1903be5a7c236a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(05)81090-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5182468$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1953719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, Gibbes R.</creatorcontrib><creatorcontrib>Saeki, Toshiaki</creatorcontrib><creatorcontrib>Auersperg, Nelly</creatorcontrib><creatorcontrib>Gordon, Alfred W.</creatorcontrib><creatorcontrib>Shoyab, Mohammed</creatorcontrib><creatorcontrib>Salomon, David S.</creatorcontrib><creatorcontrib>Stromberg, Kurt</creatorcontrib><title>Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. These results suggest that AR may play a growth regulatory role in the nucleus of cells and this role may be different in normal and malignant epithelial cells.</description><subject>Amphiregulin</subject><subject>Biological and medical sciences</subject><subject>carcinoma</subject><subject>Cell Division - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell physiology</subject><subject>EGF Family of Proteins</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epithelial Cells</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - pharmacology</subject><subject>Growth Substances - analysis</subject><subject>Growth Substances - pharmacology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Molecular and cellular biology</subject><subject>Ovarian Neoplasms</subject><subject>Ovary</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUd1qFTEYDGKpp0cfoZALEXuxmuwm2V1vRIraQlHwB7wL3ybftpHdZE12i_VZfFhzfjjFq-YmgZnJDDOEnHL2ijOuXn9ljKmibPmPl0yeNZy1rKgekdX2UXImHpPVgfKEnKT0kzHOhWqPyTFvZVXzdkX-fsE0BZ-QzoGCtxR_TxFTcsHT0FMYpxsX8XoZnKfdHQ23EB14aiAa58MIW40PcYThAKYl9mCQ4uTmGxxchgwOQ3pDPy1mQIh0CAYG9wfmvQ16G67RhyX95_iUHPUwJHy2v9fk-4f3384viqvPHy_P310VRjRqLrDrsSsVACtZLWtRC2HqVijWcKytai0DgbbnsqlqabmQhres6lBCbcpKQbUmL3b_TjH8WjDNenRpExk85ky6LkWbm5MPErniSlT5rIncEU0MKUXs9RTdCPFOc6Y38-ntfHqzjWZSb-fTG93p3mDpRrT3qt1eGX--xyHlCvsI3rh0oEnelEI1mfZ2R8Pc2q3DqJNx6A3aXK2ZtQ3ugSD_ADDKum8</recordid><startdate>19911031</startdate><enddate>19911031</enddate><creator>Johnson, Gibbes R.</creator><creator>Saeki, Toshiaki</creator><creator>Auersperg, Nelly</creator><creator>Gordon, Alfred W.</creator><creator>Shoyab, Mohammed</creator><creator>Salomon, David S.</creator><creator>Stromberg, Kurt</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911031</creationdate><title>Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin</title><author>Johnson, Gibbes R. ; Saeki, Toshiaki ; Auersperg, Nelly ; Gordon, Alfred W. ; Shoyab, Mohammed ; Salomon, David S. ; Stromberg, Kurt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-ebfeb26aa0207574744c7946081e7d69d0a4edf158375d145c1903be5a7c236a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amphiregulin</topic><topic>Biological and medical sciences</topic><topic>carcinoma</topic><topic>Cell Division - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell physiology</topic><topic>EGF Family of Proteins</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - pharmacology</topic><topic>Growth Substances - analysis</topic><topic>Growth Substances - pharmacology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Molecular and cellular biology</topic><topic>Ovarian Neoplasms</topic><topic>Ovary</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, Gibbes R.</creatorcontrib><creatorcontrib>Saeki, Toshiaki</creatorcontrib><creatorcontrib>Auersperg, Nelly</creatorcontrib><creatorcontrib>Gordon, Alfred W.</creatorcontrib><creatorcontrib>Shoyab, Mohammed</creatorcontrib><creatorcontrib>Salomon, David S.</creatorcontrib><creatorcontrib>Stromberg, Kurt</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnson, Gibbes R.</au><au>Saeki, Toshiaki</au><au>Auersperg, Nelly</au><au>Gordon, Alfred W.</au><au>Shoyab, Mohammed</au><au>Salomon, David S.</au><au>Stromberg, Kurt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-10-31</date><risdate>1991</risdate><volume>180</volume><issue>2</issue><spage>481</spage><epage>488</epage><pages>481-488</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Amphiregulin (AR) is a polypeptide growth regulator which has sequence homology to the epidermal growth factor-related family of ligands and contains putative nuclear targeting sequences. Human ovarian carcinoma cell lines and their normal counterparts, ovarian surface epithelial cells (OSEs), were assessed for their ability to respond to and express AR. Addition of exogenous AR (8–200 pM) inhibited the growth of 2 of 3 OSE specimens and 3 of the 6 carcinoma cell lines indicating that AR has the potential to inhibit the growth of normal cells, in addition to carcinoma cells. In contrast, concentrations of AR ranging from 1–5 nM stimulated the growth of all 3 of the OSEs and 4 of the 6 carcinoma cell lines. Immunocytochemical staining of the cells using antipeptide antibodies directed against residues 8–26 of AR indicated that all cells expressed AR and that the staining was localized to the nucleus. The nuclear staining of AR was concentrated in the nucleolus of the carcinoma cells, whereas the staining was diffuse in the nucleus of the OSEs. These results suggest that AR may play a growth regulatory role in the nucleus of cells and this role may be different in normal and malignant epithelial cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1953719</pmid><doi>10.1016/S0006-291X(05)81090-3</doi><tpages>8</tpages></addata></record> |
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subjects | Amphiregulin Biological and medical sciences carcinoma Cell Division - drug effects Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cell physiology EGF Family of Proteins Enzyme-Linked Immunosorbent Assay Epithelial Cells Epithelium - drug effects Epithelium - metabolism Female Fundamental and applied biological sciences. Psychology Glycoproteins - analysis Glycoproteins - pharmacology Growth Substances - analysis Growth Substances - pharmacology Humans Immunohistochemistry Intercellular Signaling Peptides and Proteins Molecular and cellular biology Ovarian Neoplasms Ovary Responses to growth factors, tumor promotors, other factors |
title | Response to and expression of amphiregulin by ovarian carcinoma and normal ovarian surface epithelial cells: Nuclear localization of endogenous amphiregulin |
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