Phylogeny and Host Specificity of Psoroptic Mange Mites (Acarina: Psoroptidae) as Indicated by ITS Sequence Data
We used molecular phylogenetic techniques to study the systematic relationships and host specificity of Psoroptes mange mites, which are pests of numerous domestic and wild ungulates. Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal...
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| Veröffentlicht in: | Journal of medical entomology 2000-11, Vol.37 (6), p.791-796 |
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| container_title | Journal of medical entomology |
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| creator | Ramey, Rob Roy Kelley, Scott Theodore Boyce, Walter Miles Dorsey Farrell, Brian |
| description | We used molecular phylogenetic techniques to study the systematic relationships and host specificity of Psoroptes mange mites, which are pests of numerous domestic and wild ungulates. Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal DNA indicated that populations of Psoroptes are not host specific. Furthermore, the currently used taxonomy of Psoroptes is not concordant with the phylogeny derived from ITS1. During the course of the study, we discovered apparent paralogous ITS sequences within individual mites as a result of varying polymerase chain reaction reaction conditions. This finding concords with other studies of ITS and suggests a cautious approach when interpreting data from ITS sequences. Host DNA contamination was also found to be a significant problem in data collection, and we report on the development of methods to overcome the problems of contamination in parasitic mites. |
| doi_str_mv | 10.1603/0022-2585-37.6.791 |
| format | Article |
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Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal DNA indicated that populations of Psoroptes are not host specific. Furthermore, the currently used taxonomy of Psoroptes is not concordant with the phylogeny derived from ITS1. During the course of the study, we discovered apparent paralogous ITS sequences within individual mites as a result of varying polymerase chain reaction reaction conditions. This finding concords with other studies of ITS and suggests a cautious approach when interpreting data from ITS sequences. Host DNA contamination was also found to be a significant problem in data collection, and we report on the development of methods to overcome the problems of contamination in parasitic mites.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1603/0022-2585-37.6.791</identifier><identifier>PMID: 11126531</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Analysis ; Animals ; Arachnida ; bighorn sheep ; Biological and medical sciences ; DNA sequencing ; DNA, Ribosomal Spacer ; Fundamental and applied biological sciences. Psychology ; internal transcriber spacer region ; Invertebrates ; Mites - classification ; Mites - genetics ; Nucleotide sequencing ; parasitism ; Phylogeny ; Psoroptes ; Systematics. 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Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal DNA indicated that populations of Psoroptes are not host specific. Furthermore, the currently used taxonomy of Psoroptes is not concordant with the phylogeny derived from ITS1. During the course of the study, we discovered apparent paralogous ITS sequences within individual mites as a result of varying polymerase chain reaction reaction conditions. This finding concords with other studies of ITS and suggests a cautious approach when interpreting data from ITS sequences. Host DNA contamination was also found to be a significant problem in data collection, and we report on the development of methods to overcome the problems of contamination in parasitic mites.</description><subject>Analysis</subject><subject>Animals</subject><subject>Arachnida</subject><subject>bighorn sheep</subject><subject>Biological and medical sciences</subject><subject>DNA sequencing</subject><subject>DNA, Ribosomal Spacer</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>internal transcriber spacer region</subject><subject>Invertebrates</subject><subject>Mites - classification</subject><subject>Mites - genetics</subject><subject>Nucleotide sequencing</subject><subject>parasitism</subject><subject>Phylogeny</subject><subject>Psoroptes</subject><subject>Systematics. 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Psychology</topic><topic>internal transcriber spacer region</topic><topic>Invertebrates</topic><topic>Mites - classification</topic><topic>Mites - genetics</topic><topic>Nucleotide sequencing</topic><topic>parasitism</topic><topic>Phylogeny</topic><topic>Psoroptes</topic><topic>Systematics. Geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramey, Rob Roy</creatorcontrib><creatorcontrib>Kelley, Scott Theodore</creatorcontrib><creatorcontrib>Boyce, Walter Miles</creatorcontrib><creatorcontrib>Dorsey Farrell, Brian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramey, Rob Roy</au><au>Kelley, Scott Theodore</au><au>Boyce, Walter Miles</au><au>Dorsey Farrell, Brian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogeny and Host Specificity of Psoroptic Mange Mites (Acarina: Psoroptidae) as Indicated by ITS Sequence Data</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2000-11</date><risdate>2000</risdate><volume>37</volume><issue>6</issue><spage>791</spage><epage>796</epage><pages>791-796</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>We used molecular phylogenetic techniques to study the systematic relationships and host specificity of Psoroptes mange mites, which are pests of numerous domestic and wild ungulates. Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal DNA indicated that populations of Psoroptes are not host specific. Furthermore, the currently used taxonomy of Psoroptes is not concordant with the phylogeny derived from ITS1. During the course of the study, we discovered apparent paralogous ITS sequences within individual mites as a result of varying polymerase chain reaction reaction conditions. This finding concords with other studies of ITS and suggests a cautious approach when interpreting data from ITS sequences. Host DNA contamination was also found to be a significant problem in data collection, and we report on the development of methods to overcome the problems of contamination in parasitic mites.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>11126531</pmid><doi>10.1603/0022-2585-37.6.791</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of medical entomology, 2000-11, Vol.37 (6), p.791-796 |
| issn | 0022-2585 1938-2928 |
| language | eng |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Analysis Animals Arachnida bighorn sheep Biological and medical sciences DNA sequencing DNA, Ribosomal Spacer Fundamental and applied biological sciences. Psychology internal transcriber spacer region Invertebrates Mites - classification Mites - genetics Nucleotide sequencing parasitism Phylogeny Psoroptes Systematics. Geographical distribution |
| title | Phylogeny and Host Specificity of Psoroptic Mange Mites (Acarina: Psoroptidae) as Indicated by ITS Sequence Data |
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