The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation
The influence of estrogen (E) and antiestrogen (AES) on the in vitro growth of BG-1 ovarian carcinoma cells, which express steroid receptors was examined (K. R. Geisinger, T. E. Kute, M. J. Pettenati, C. E. Welander, Y. Dennard, L. A. Collins, and M. E. Berens, Characterization of a human ovarian ca...
Gespeichert in:
| Veröffentlicht in: | Gynecologic oncology 1991-09, Vol.42 (3), p.245-249 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 249 |
|---|---|
| container_issue | 3 |
| container_start_page | 245 |
| container_title | Gynecologic oncology |
| container_volume | 42 |
| creator | Pavlik, Edward J. Nelson, Katherine van Nagell, John R. Gallion, Holly S. Donaldson, Elvis S. DePriest, P. Meares, Katherine van Nagell, John R. |
| description | The influence of estrogen (E) and antiestrogen (AES) on the
in vitro growth of BG-1 ovarian carcinoma cells, which express steroid receptors was examined (K. R. Geisinger, T. E. Kute, M. J. Pettenati, C. E. Welander, Y. Dennard, L. A. Collins, and M. E. Berens, Characterization of a human ovarian carcinoma cell line with estrogen and progesterone receptors,
Cancer 63, 280–288, 1989). All determinations were simultaneously referenced under similar conditions to MCF-7 cells, a well-established cell line for modeling hormonal responses in breast cancer. In “complete” media containing fetal calf serum (FCS, 10%), MCF-7 cell numbers increased ~7× in 7 days, remaining at this level Days 8–15. In contrast, BG-1 cells achieved similar numbers by Day 7, but showed apparent exponential growth over Days 8–15 to 15–20 ×. Phenol red-free media containing 10% FCS ( |
| doi_str_mv | 10.1016/0090-8258(91)90353-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72489442</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0090825891903537</els_id><sourcerecordid>72489442</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-8e3bc133dab270af2abdef64effb9a14e7930ad9c830c72aacc643a054e29c3b3</originalsourceid><addsrcrecordid>eNp9UctOHDEQtBAR2ZD8AZF8QkRiEr9mZ8wBKUEEIiFxIWerx26D0Y692N5N8hP55sxkEbnl1GpVdXV3FSFHnH3kjC8_MaZZ04u2P9H8g2aylU23Rxac6bZZ9q3eJ4sXymvyppRHxphkXByQA67blvfdgvy-e0B6n9OP-kAzlnWKBWny9MtVw2naQg4QqYVsQ0wjUIurVaE1USw1gwtpdUrV7XVTYUw_g8d4SiE6-tKe0cttcBgtUp8yDbHmEEuwE6uGWSPdY6Rga9hCDSm-Ja88rAq-e66H5PvXy7uL6-bm9urbxeebxopO1KZHOVgupYNBdAy8gMGhXyr0ftDAFXZaMnDa9pLZTgBYu1QSWKtQaCsHeUiOd7rrnJ420yFmDGV-DiKmTTGdUL1WSkxEtSPanErJ6M06hxHyL8OZmWMws8dm9thobv7GYLpp7P2z_mYY0f0b2vk-4ec7HKcntwGzKTbMNrmQ0VbjUvj_gj95lZna</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72489442</pqid></control><display><type>article</type><title>The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Pavlik, Edward J. ; Nelson, Katherine ; van Nagell, John R. ; Gallion, Holly S. ; Donaldson, Elvis S. ; DePriest, P. ; Meares, Katherine ; van Nagell, John R.</creator><creatorcontrib>Pavlik, Edward J. ; Nelson, Katherine ; van Nagell, John R. ; Gallion, Holly S. ; Donaldson, Elvis S. ; DePriest, P. ; Meares, Katherine ; van Nagell, John R.</creatorcontrib><description>The influence of estrogen (E) and antiestrogen (AES) on the
in vitro growth of BG-1 ovarian carcinoma cells, which express steroid receptors was examined (K. R. Geisinger, T. E. Kute, M. J. Pettenati, C. E. Welander, Y. Dennard, L. A. Collins, and M. E. Berens, Characterization of a human ovarian carcinoma cell line with estrogen and progesterone receptors,
Cancer 63, 280–288, 1989). All determinations were simultaneously referenced under similar conditions to MCF-7 cells, a well-established cell line for modeling hormonal responses in breast cancer. In “complete” media containing fetal calf serum (FCS, 10%), MCF-7 cell numbers increased ~7× in 7 days, remaining at this level Days 8–15. In contrast, BG-1 cells achieved similar numbers by Day 7, but showed apparent exponential growth over Days 8–15 to 15–20 ×. Phenol red-free media containing 10% FCS (<20 pg estradiol (E
2)/ml by RIA) was used to assess responses to E and AES. Growth of both MCF-7 and BG-1 cells slowed in E-free media. E
2 (10 n
M) stimulated the growth of both cell lines, yet was responsible for exponential increases during Days 8–15 only in BG-1 cell numbers (50–70 ×). The metabolically active AES (4OH-tamoxifen, 50 n
M) reduced E
2-stimulated MCF-7 growth to 3–4 ×, while tamoxifen (50 n
M) had no effect. Rescue with 10
μM E
2 fully overcame the AES inhibition of MCF-7 proliferation. In contrast, BG-1 cells experienced significant E
2-stimulated growth reductions in the presence of either 4OH-tamoxifen or tamoxifen. E
2 was observed to rescue BG-1 cells from
both of these antagonists. We conclude that BG-1 ovarian carcinoma cells respond
in vitro to E and AES. Moreover, by virtue of responses to tamoxifen, BG-1 cells may have an intrinsic capacity to hydroxylate tamoxifen to its active metabolite. This property of ovarian carcinoma cells might be worth exploiting in the design of more effective combination chemotherapy regimens.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1016/0090-8258(91)90353-7</identifier><identifier>PMID: 1955187</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Carcinoma - pathology ; Cell Division - drug effects ; Culture Media ; Estradiol - pharmacology ; Estrogen Antagonists - pharmacology ; Estrogens - pharmacology ; Female ; Humans ; Ovarian Neoplasms - pathology ; Tamoxifen - analogs & derivatives ; Tamoxifen - pharmacology ; Tumor Cells, Cultured</subject><ispartof>Gynecologic oncology, 1991-09, Vol.42 (3), p.245-249</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-8e3bc133dab270af2abdef64effb9a14e7930ad9c830c72aacc643a054e29c3b3</citedby><cites>FETCH-LOGICAL-c272t-8e3bc133dab270af2abdef64effb9a14e7930ad9c830c72aacc643a054e29c3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0090-8258(91)90353-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1955187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pavlik, Edward J.</creatorcontrib><creatorcontrib>Nelson, Katherine</creatorcontrib><creatorcontrib>van Nagell, John R.</creatorcontrib><creatorcontrib>Gallion, Holly S.</creatorcontrib><creatorcontrib>Donaldson, Elvis S.</creatorcontrib><creatorcontrib>DePriest, P.</creatorcontrib><creatorcontrib>Meares, Katherine</creatorcontrib><creatorcontrib>van Nagell, John R.</creatorcontrib><title>The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>The influence of estrogen (E) and antiestrogen (AES) on the
in vitro growth of BG-1 ovarian carcinoma cells, which express steroid receptors was examined (K. R. Geisinger, T. E. Kute, M. J. Pettenati, C. E. Welander, Y. Dennard, L. A. Collins, and M. E. Berens, Characterization of a human ovarian carcinoma cell line with estrogen and progesterone receptors,
Cancer 63, 280–288, 1989). All determinations were simultaneously referenced under similar conditions to MCF-7 cells, a well-established cell line for modeling hormonal responses in breast cancer. In “complete” media containing fetal calf serum (FCS, 10%), MCF-7 cell numbers increased ~7× in 7 days, remaining at this level Days 8–15. In contrast, BG-1 cells achieved similar numbers by Day 7, but showed apparent exponential growth over Days 8–15 to 15–20 ×. Phenol red-free media containing 10% FCS (<20 pg estradiol (E
2)/ml by RIA) was used to assess responses to E and AES. Growth of both MCF-7 and BG-1 cells slowed in E-free media. E
2 (10 n
M) stimulated the growth of both cell lines, yet was responsible for exponential increases during Days 8–15 only in BG-1 cell numbers (50–70 ×). The metabolically active AES (4OH-tamoxifen, 50 n
M) reduced E
2-stimulated MCF-7 growth to 3–4 ×, while tamoxifen (50 n
M) had no effect. Rescue with 10
μM E
2 fully overcame the AES inhibition of MCF-7 proliferation. In contrast, BG-1 cells experienced significant E
2-stimulated growth reductions in the presence of either 4OH-tamoxifen or tamoxifen. E
2 was observed to rescue BG-1 cells from
both of these antagonists. We conclude that BG-1 ovarian carcinoma cells respond
in vitro to E and AES. Moreover, by virtue of responses to tamoxifen, BG-1 cells may have an intrinsic capacity to hydroxylate tamoxifen to its active metabolite. This property of ovarian carcinoma cells might be worth exploiting in the design of more effective combination chemotherapy regimens.</description><subject>Carcinoma - pathology</subject><subject>Cell Division - drug effects</subject><subject>Culture Media</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Estrogens - pharmacology</subject><subject>Female</subject><subject>Humans</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Tamoxifen - analogs & derivatives</subject><subject>Tamoxifen - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UctOHDEQtBAR2ZD8AZF8QkRiEr9mZ8wBKUEEIiFxIWerx26D0Y692N5N8hP55sxkEbnl1GpVdXV3FSFHnH3kjC8_MaZZ04u2P9H8g2aylU23Rxac6bZZ9q3eJ4sXymvyppRHxphkXByQA67blvfdgvy-e0B6n9OP-kAzlnWKBWny9MtVw2naQg4QqYVsQ0wjUIurVaE1USw1gwtpdUrV7XVTYUw_g8d4SiE6-tKe0cttcBgtUp8yDbHmEEuwE6uGWSPdY6Rga9hCDSm-Ja88rAq-e66H5PvXy7uL6-bm9urbxeebxopO1KZHOVgupYNBdAy8gMGhXyr0ftDAFXZaMnDa9pLZTgBYu1QSWKtQaCsHeUiOd7rrnJ420yFmDGV-DiKmTTGdUL1WSkxEtSPanErJ6M06hxHyL8OZmWMws8dm9thobv7GYLpp7P2z_mYY0f0b2vk-4ec7HKcntwGzKTbMNrmQ0VbjUvj_gj95lZna</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>Pavlik, Edward J.</creator><creator>Nelson, Katherine</creator><creator>van Nagell, John R.</creator><creator>Gallion, Holly S.</creator><creator>Donaldson, Elvis S.</creator><creator>DePriest, P.</creator><creator>Meares, Katherine</creator><creator>van Nagell, John R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199109</creationdate><title>The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation</title><author>Pavlik, Edward J. ; Nelson, Katherine ; van Nagell, John R. ; Gallion, Holly S. ; Donaldson, Elvis S. ; DePriest, P. ; Meares, Katherine ; van Nagell, John R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-8e3bc133dab270af2abdef64effb9a14e7930ad9c830c72aacc643a054e29c3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Carcinoma - pathology</topic><topic>Cell Division - drug effects</topic><topic>Culture Media</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Estrogens - pharmacology</topic><topic>Female</topic><topic>Humans</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Tamoxifen - analogs & derivatives</topic><topic>Tamoxifen - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pavlik, Edward J.</creatorcontrib><creatorcontrib>Nelson, Katherine</creatorcontrib><creatorcontrib>van Nagell, John R.</creatorcontrib><creatorcontrib>Gallion, Holly S.</creatorcontrib><creatorcontrib>Donaldson, Elvis S.</creatorcontrib><creatorcontrib>DePriest, P.</creatorcontrib><creatorcontrib>Meares, Katherine</creatorcontrib><creatorcontrib>van Nagell, John R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pavlik, Edward J.</au><au>Nelson, Katherine</au><au>van Nagell, John R.</au><au>Gallion, Holly S.</au><au>Donaldson, Elvis S.</au><au>DePriest, P.</au><au>Meares, Katherine</au><au>van Nagell, John R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>1991-09</date><risdate>1991</risdate><volume>42</volume><issue>3</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><abstract>The influence of estrogen (E) and antiestrogen (AES) on the
in vitro growth of BG-1 ovarian carcinoma cells, which express steroid receptors was examined (K. R. Geisinger, T. E. Kute, M. J. Pettenati, C. E. Welander, Y. Dennard, L. A. Collins, and M. E. Berens, Characterization of a human ovarian carcinoma cell line with estrogen and progesterone receptors,
Cancer 63, 280–288, 1989). All determinations were simultaneously referenced under similar conditions to MCF-7 cells, a well-established cell line for modeling hormonal responses in breast cancer. In “complete” media containing fetal calf serum (FCS, 10%), MCF-7 cell numbers increased ~7× in 7 days, remaining at this level Days 8–15. In contrast, BG-1 cells achieved similar numbers by Day 7, but showed apparent exponential growth over Days 8–15 to 15–20 ×. Phenol red-free media containing 10% FCS (<20 pg estradiol (E
2)/ml by RIA) was used to assess responses to E and AES. Growth of both MCF-7 and BG-1 cells slowed in E-free media. E
2 (10 n
M) stimulated the growth of both cell lines, yet was responsible for exponential increases during Days 8–15 only in BG-1 cell numbers (50–70 ×). The metabolically active AES (4OH-tamoxifen, 50 n
M) reduced E
2-stimulated MCF-7 growth to 3–4 ×, while tamoxifen (50 n
M) had no effect. Rescue with 10
μM E
2 fully overcame the AES inhibition of MCF-7 proliferation. In contrast, BG-1 cells experienced significant E
2-stimulated growth reductions in the presence of either 4OH-tamoxifen or tamoxifen. E
2 was observed to rescue BG-1 cells from
both of these antagonists. We conclude that BG-1 ovarian carcinoma cells respond
in vitro to E and AES. Moreover, by virtue of responses to tamoxifen, BG-1 cells may have an intrinsic capacity to hydroxylate tamoxifen to its active metabolite. This property of ovarian carcinoma cells might be worth exploiting in the design of more effective combination chemotherapy regimens.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1955187</pmid><doi>10.1016/0090-8258(91)90353-7</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-8258 |
| ispartof | Gynecologic oncology, 1991-09, Vol.42 (3), p.245-249 |
| issn | 0090-8258 1095-6859 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72489442 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Carcinoma - pathology Cell Division - drug effects Culture Media Estradiol - pharmacology Estrogen Antagonists - pharmacology Estrogens - pharmacology Female Humans Ovarian Neoplasms - pathology Tamoxifen - analogs & derivatives Tamoxifen - pharmacology Tumor Cells, Cultured |
| title | The growth response of BG-1 ovarian carcinoma cells to estradiol, 4OH-tamoxifen, and tamoxifen: Evidence for intrinsic antiestrogen activation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T09%3A42%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20growth%20response%20of%20BG-1%20ovarian%20carcinoma%20cells%20to%20estradiol,%204OH-tamoxifen,%20and%20tamoxifen:%20Evidence%20for%20intrinsic%20antiestrogen%20activation&rft.jtitle=Gynecologic%20oncology&rft.au=Pavlik,%20Edward%20J.&rft.date=1991-09&rft.volume=42&rft.issue=3&rft.spage=245&rft.epage=249&rft.pages=245-249&rft.issn=0090-8258&rft.eissn=1095-6859&rft_id=info:doi/10.1016/0090-8258(91)90353-7&rft_dat=%3Cproquest_cross%3E72489442%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72489442&rft_id=info:pmid/1955187&rft_els_id=0090825891903537&rfr_iscdi=true |