Genomic Exploration of the Hemiascomycetous Yeasts: 2. Data generation and processing
The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasm...
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| Veröffentlicht in: | FEBS letters 2000-12, Vol.487 (1), p.13-16 |
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| container_title | FEBS letters |
| container_volume | 487 |
| creator | Artiguenave, François Wincker, Patrick Brottier, Phillippe Duprat, Simone Jovelin, Fabien Scarpelli, Claude Verdier, Jean Vico, Virginie Weissenbach, Jean Saurin, William |
| description | The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length. |
| doi_str_mv | 10.1016/S0014-5793(00)02273-0 |
| format | Article |
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| ispartof | FEBS letters, 2000-12, Vol.487 (1), p.13-16 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | Wiley Free Content; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Ascomycota - genetics Computational Biology - methods Genome Genome, Fungal Hemiascomycete High-throughput sequencing Pichia - genetics Sequence analysis Sequence Analysis, DNA - methods |
| title | Genomic Exploration of the Hemiascomycetous Yeasts: 2. Data generation and processing |
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