DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication
Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for Autographa califomica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Wes...
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| Veröffentlicht in: | Virus research 1991-07, Vol.20 (2), p.133-145 |
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| creator | McClintock, J.Thomas Guzo, Dave Guthrie, Kim P. Dougherty, Edward M. |
| description | Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for
Autographa califomica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots containing uninfected and infected cell proteins with AcMNPV or host DNA probes. In the AcMNPV-infected TN-368 permissive cell system, 8 virus-induced DNA-binding proteins with molecular weights ranging from 67.5K to 18.75K were observed under the highest conditions of stringency. When these DNA-binding proteins were compared to structural proteins of AcMNPV, several appeared to be similar to those observed in SDS-PAGE protein profiles of nonoccluded virus (NOV) and occlusion body (PIBs) preparations. Using an AcMNPV occlusion negative mutant (L1GP-
gal3) and an anti-AcMNPV-polyhedrin monoclonal antibody, a major DNA-binding protein (33.0K), observed in the permissive system, corresponded to polyhedrin and to a comigrating virus-induced DNA-binding protein. In the AcMNPV-infected IPLB-LD-652Y semipermissive cell system, no virus-induced DNA-binding proteins were detected. However, several host DNA-binding proteins were present but their ability to bind DNA decreased significantly following infection. |
| doi_str_mv | 10.1016/0168-1702(91)90105-5 |
| format | Article |
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Autographa califomica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots containing uninfected and infected cell proteins with AcMNPV or host DNA probes. In the AcMNPV-infected TN-368 permissive cell system, 8 virus-induced DNA-binding proteins with molecular weights ranging from 67.5K to 18.75K were observed under the highest conditions of stringency. When these DNA-binding proteins were compared to structural proteins of AcMNPV, several appeared to be similar to those observed in SDS-PAGE protein profiles of nonoccluded virus (NOV) and occlusion body (PIBs) preparations. Using an AcMNPV occlusion negative mutant (L1GP-
gal3) and an anti-AcMNPV-polyhedrin monoclonal antibody, a major DNA-binding protein (33.0K), observed in the permissive system, corresponded to polyhedrin and to a comigrating virus-induced DNA-binding protein. In the AcMNPV-infected IPLB-LD-652Y semipermissive cell system, no virus-induced DNA-binding proteins were detected. However, several host DNA-binding proteins were present but their ability to bind DNA decreased significantly following infection.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/0168-1702(91)90105-5</identifier><identifier>PMID: 1950170</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Autographa californica ; Baculoviridae - metabolism ; Baculovirus ; Cell Line ; DNA-binding proteins ; DNA-Binding Proteins - isolation & purification ; DNA-Binding Proteins - metabolism ; Electrophoresis, Polyacrylamide Gel ; nuclear polyhedrosis virus ; Permissive ; Semipermissive ; Viral Proteins - isolation & purification ; Viral Proteins - metabolism ; Virus Replication</subject><ispartof>Virus research, 1991-07, Vol.20 (2), p.133-145</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c337t-7dd127c475f8054b006235ab76853a64d4f3215d450bb0273bd2b003939a358d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1702(91)90105-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1950170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McClintock, J.Thomas</creatorcontrib><creatorcontrib>Guzo, Dave</creatorcontrib><creatorcontrib>Guthrie, Kim P.</creatorcontrib><creatorcontrib>Dougherty, Edward M.</creatorcontrib><title>DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for
Autographa califomica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots containing uninfected and infected cell proteins with AcMNPV or host DNA probes. In the AcMNPV-infected TN-368 permissive cell system, 8 virus-induced DNA-binding proteins with molecular weights ranging from 67.5K to 18.75K were observed under the highest conditions of stringency. When these DNA-binding proteins were compared to structural proteins of AcMNPV, several appeared to be similar to those observed in SDS-PAGE protein profiles of nonoccluded virus (NOV) and occlusion body (PIBs) preparations. Using an AcMNPV occlusion negative mutant (L1GP-
gal3) and an anti-AcMNPV-polyhedrin monoclonal antibody, a major DNA-binding protein (33.0K), observed in the permissive system, corresponded to polyhedrin and to a comigrating virus-induced DNA-binding protein. In the AcMNPV-infected IPLB-LD-652Y semipermissive cell system, no virus-induced DNA-binding proteins were detected. However, several host DNA-binding proteins were present but their ability to bind DNA decreased significantly following infection.</description><subject>Animals</subject><subject>Autographa californica</subject><subject>Baculoviridae - metabolism</subject><subject>Baculovirus</subject><subject>Cell Line</subject><subject>DNA-binding proteins</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>nuclear polyhedrosis virus</subject><subject>Permissive</subject><subject>Semipermissive</subject><subject>Viral Proteins - isolation & purification</subject><subject>Viral Proteins - metabolism</subject><subject>Virus Replication</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LxDAQhoMo67r6DxR6Ej1U89E07UVY1k9Y9KLnkCZTiXTTNWkX_PemW1FPehgGZp6ZvHkHoWOCLwgm-WWMIiUC07OSnJeYYJ7yHTQlhaCpyEq6i6bfyD46COENY5wzkU_QhJQcx_oU6evHeVpZZ6x7Tda-7cC6kLR1UindN-3G-j6k1tWgOzCJhqYJien9lga_siHYDSTKmSTAyv4qeVg3VqvOtu4Q7dWqCXD0lWfo5fbmeXGfLp_uHhbzZaoZE10qjCFU6EzwusA8q6JYyriqRF5wpvLMZDWjhJuM46rCVLDK0AixkpWK8cKwGTod98Z_vPcQOhnFDJKVg7YPUtCsYIKzf0GSM8ZwjiOYjaD2bQgearn2dqX8hyRYDkeQg8NycFiWRG6PIHkcO_na31crMD9Do-uxfzX2IbqxseBl0BacBmN9NFqa1v79wCeorpYc</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>McClintock, J.Thomas</creator><creator>Guzo, Dave</creator><creator>Guthrie, Kim P.</creator><creator>Dougherty, Edward M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910701</creationdate><title>DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication</title><author>McClintock, J.Thomas ; Guzo, Dave ; Guthrie, Kim P. ; Dougherty, Edward M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-7dd127c475f8054b006235ab76853a64d4f3215d450bb0273bd2b003939a358d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Autographa californica</topic><topic>Baculoviridae - metabolism</topic><topic>Baculovirus</topic><topic>Cell Line</topic><topic>DNA-binding proteins</topic><topic>DNA-Binding Proteins - isolation & purification</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>nuclear polyhedrosis virus</topic><topic>Permissive</topic><topic>Semipermissive</topic><topic>Viral Proteins - isolation & purification</topic><topic>Viral Proteins - metabolism</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McClintock, J.Thomas</creatorcontrib><creatorcontrib>Guzo, Dave</creatorcontrib><creatorcontrib>Guthrie, Kim P.</creatorcontrib><creatorcontrib>Dougherty, Edward M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McClintock, J.Thomas</au><au>Guzo, Dave</au><au>Guthrie, Kim P.</au><au>Dougherty, Edward M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>20</volume><issue>2</issue><spage>133</spage><epage>145</epage><pages>133-145</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for
Autographa califomica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots containing uninfected and infected cell proteins with AcMNPV or host DNA probes. In the AcMNPV-infected TN-368 permissive cell system, 8 virus-induced DNA-binding proteins with molecular weights ranging from 67.5K to 18.75K were observed under the highest conditions of stringency. When these DNA-binding proteins were compared to structural proteins of AcMNPV, several appeared to be similar to those observed in SDS-PAGE protein profiles of nonoccluded virus (NOV) and occlusion body (PIBs) preparations. Using an AcMNPV occlusion negative mutant (L1GP-
gal3) and an anti-AcMNPV-polyhedrin monoclonal antibody, a major DNA-binding protein (33.0K), observed in the permissive system, corresponded to polyhedrin and to a comigrating virus-induced DNA-binding protein. In the AcMNPV-infected IPLB-LD-652Y semipermissive cell system, no virus-induced DNA-binding proteins were detected. However, several host DNA-binding proteins were present but their ability to bind DNA decreased significantly following infection.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>1950170</pmid><doi>10.1016/0168-1702(91)90105-5</doi><tpages>13</tpages></addata></record> |
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| ispartof | Virus research, 1991-07, Vol.20 (2), p.133-145 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Autographa californica Baculoviridae - metabolism Baculovirus Cell Line DNA-binding proteins DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Electrophoresis, Polyacrylamide Gel nuclear polyhedrosis virus Permissive Semipermissive Viral Proteins - isolation & purification Viral Proteins - metabolism Virus Replication |
| title | DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication |
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