MEKK1 Binds Raf-1 and the ERK2 Cascade Components
Mitogen-activated protein (MAP) kinase cascades are involved in transmitting signals that are generated at the cell surface into the cytosol and nucleus and consist of three sequentially acting enzymes: a MAP kinase, an upstream MAP/extracellular signal-regulated protein kinase (ERK) kinase (MEK), a...
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| Veröffentlicht in: | The Journal of biological chemistry 2000-12, Vol.275 (51), p.40120-40127 |
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| container_issue | 51 |
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| container_title | The Journal of biological chemistry |
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| creator | Karandikar, Mahesh Xu, Shuichan Cobb, Melanie H. |
| description | Mitogen-activated protein (MAP) kinase cascades are involved in transmitting signals that are generated at the cell surface into the cytosol and nucleus and consist of three sequentially acting enzymes: a MAP kinase, an upstream MAP/extracellular signal-regulated protein kinase (ERK) kinase (MEK), and a MEK kinase (MEKK). Protein-protein interactions within these cascades provide a mechanism to control the localization and function of the proteins. MEKK1 is implicated in activation of the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and ERK1/2 MAP kinase pathways. We showed previously that MEKK1 binds directly to JNK/SAPK. In this study we demonstrate that endogenous MEKK1 binds to endogenous ERK2, MEK1, and another MEKK level kinase, Raf-1, suggesting that it can assemble all three proteins of the ERK2 MAP kinase module. |
| doi_str_mv | 10.1074/jbc.M005926200 |
| format | Article |
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Protein-protein interactions within these cascades provide a mechanism to control the localization and function of the proteins. MEKK1 is implicated in activation of the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and ERK1/2 MAP kinase pathways. We showed previously that MEKK1 binds directly to JNK/SAPK. 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| ispartof | The Journal of biological chemistry, 2000-12, Vol.275 (51), p.40120-40127 |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Cell Line Humans MAP Kinase Kinase Kinase 1 MAP Kinase Signaling System Mitogen-Activated Protein Kinase 1 - metabolism Protein Binding Protein-Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins c-raf - metabolism Substrate Specificity |
| title | MEKK1 Binds Raf-1 and the ERK2 Cascade Components |
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