Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography
Enantiomeric determination of L‐ and D‐lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L‐ and D‐lactate in the extract were sepa...
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| Veröffentlicht in: | Biomedical chromatography 2000-11, Vol.14 (7), p.474-477 |
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| creator | Okubo, Shigeo Mashige, Fumiko Omori, Mie Hashimoto, Yoshiaki Nakahara, Kazuhiko Kanazawa, Hideko Matsushima, Yoshikazu |
| description | Enantiomeric determination of L‐ and D‐lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L‐ and D‐lactate in the extract were separated by Shodex ORpac CRX‐453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L‐ and D‐lactate were determined in 25 min. Intra‐assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L‐lactate and 10.1% (mean 4.96 µmol/L) for D‐lactate (n = 5). Detection limits were between 1.0 (L‐lactate) and 1.5 (D‐lactate) pmol. The mean values (n = 3) of analytical recovery for L‐ and D‐lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L‐ and D‐lactate in CSF (n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 µmol/L, respectively. Copyright © 2000 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/1099-0801(200011)14:7<474::AID-BMC995>3.0.CO;2-3 |
| format | Article |
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Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L‐ and D‐lactate in the extract were separated by Shodex ORpac CRX‐453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L‐ and D‐lactate were determined in 25 min. Intra‐assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L‐lactate and 10.1% (mean 4.96 µmol/L) for D‐lactate (n = 5). Detection limits were between 1.0 (L‐lactate) and 1.5 (D‐lactate) pmol. The mean values (n = 3) of analytical recovery for L‐ and D‐lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L‐ and D‐lactate in CSF (n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 µmol/L, respectively. Copyright © 2000 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/1099-0801(200011)14:7<474::AID-BMC995>3.0.CO;2-3</identifier><identifier>PMID: 11113927</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Calibration ; Chromatography, High Pressure Liquid - methods ; Humans ; Lactic Acid - cerebrospinal fluid ; Ligands ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrophotometry, Ultraviolet ; Stereoisomerism</subject><ispartof>Biomedical chromatography, 2000-11, Vol.14 (7), p.474-477</ispartof><rights>Copyright © 2000 John Wiley & Sons, Ltd.</rights><rights>Copyright 2000 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1099-0801%28200011%2914%3A7%3C474%3A%3AAID-BMC995%3E3.0.CO%3B2-3$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1099-0801%28200011%2914%3A7%3C474%3A%3AAID-BMC995%3E3.0.CO%3B2-3$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11113927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okubo, Shigeo</creatorcontrib><creatorcontrib>Mashige, Fumiko</creatorcontrib><creatorcontrib>Omori, Mie</creatorcontrib><creatorcontrib>Hashimoto, Yoshiaki</creatorcontrib><creatorcontrib>Nakahara, Kazuhiko</creatorcontrib><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Matsushima, Yoshikazu</creatorcontrib><title>Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>Enantiomeric determination of L‐ and D‐lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L‐ and D‐lactate in the extract were separated by Shodex ORpac CRX‐453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L‐ and D‐lactate were determined in 25 min. Intra‐assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L‐lactate and 10.1% (mean 4.96 µmol/L) for D‐lactate (n = 5). Detection limits were between 1.0 (L‐lactate) and 1.5 (D‐lactate) pmol. The mean values (n = 3) of analytical recovery for L‐ and D‐lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L‐ and D‐lactate in CSF (n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 µmol/L, respectively. Copyright © 2000 John Wiley & Sons, Ltd.</description><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Humans</subject><subject>Lactic Acid - cerebrospinal fluid</subject><subject>Ligands</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Stereoisomerism</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd1u1DAQhS0EokvhFZCvEFx4seNkbS8IqWxLabVlLyg_4mbkOPbGkL86iei-BY-Mo5QyNx7PnHMu5kNIMrpklCavGVWKUEnZy4RSytgrlq7F21Sk6_XJxSl5f7VRKnvHl3S52b1JCH-AFveWh2hBk5UiXAp1hJ70_c8YoVaJeIyOWCyuErFAf84a3Qy-rW3wBhd2sKH2jY6TBrcObwnWTYFPSaXNEAXa-AL7BpdjrRtsbLB5aPsuOirsqjEu8wM2pQ_xX_n95LW3ptTN3uLS70vS2eDaEM3GRsHN5DBlaGs9tPugu_LwFD1yuurts7v3GH35cHa9-Ui2u_OLzcmWeJ6wjBhqlLV6xTInpWEqLbjlzvGskC7j8SqqUAnPcpE7w10qrJGucEbk0qhcKcmP0Ys5twvtzWj7AWrfG1tVurHt2INIUiFplkbh8zvhmNe2gC74WocD_LthFHyeBb99ZQ__9xQmhjABgQkIzAyBpSAgMoSIEGaEwIHCZgdJbOZJTCVzqu8He3ufqsMvWAkuMvj26Ryu-ZW8_PH9K1zyvxm0pQw</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Okubo, Shigeo</creator><creator>Mashige, Fumiko</creator><creator>Omori, Mie</creator><creator>Hashimoto, Yoshiaki</creator><creator>Nakahara, Kazuhiko</creator><creator>Kanazawa, Hideko</creator><creator>Matsushima, Yoshikazu</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200011</creationdate><title>Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography</title><author>Okubo, Shigeo ; Mashige, Fumiko ; Omori, Mie ; Hashimoto, Yoshiaki ; Nakahara, Kazuhiko ; Kanazawa, Hideko ; Matsushima, Yoshikazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3215-c0c9eea615f88c194d3e3ff35d8f539959d9235b7bfc3f47ec8fdfc7b8c9b9983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Humans</topic><topic>Lactic Acid - cerebrospinal fluid</topic><topic>Ligands</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okubo, Shigeo</creatorcontrib><creatorcontrib>Mashige, Fumiko</creatorcontrib><creatorcontrib>Omori, Mie</creatorcontrib><creatorcontrib>Hashimoto, Yoshiaki</creatorcontrib><creatorcontrib>Nakahara, Kazuhiko</creatorcontrib><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Matsushima, Yoshikazu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okubo, Shigeo</au><au>Mashige, Fumiko</au><au>Omori, Mie</au><au>Hashimoto, Yoshiaki</au><au>Nakahara, Kazuhiko</au><au>Kanazawa, Hideko</au><au>Matsushima, Yoshikazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2000-11</date><risdate>2000</risdate><volume>14</volume><issue>7</issue><spage>474</spage><epage>477</epage><pages>474-477</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>Enantiomeric determination of L‐ and D‐lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L‐ and D‐lactate in the extract were separated by Shodex ORpac CRX‐453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L‐ and D‐lactate were determined in 25 min. Intra‐assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L‐lactate and 10.1% (mean 4.96 µmol/L) for D‐lactate (n = 5). Detection limits were between 1.0 (L‐lactate) and 1.5 (D‐lactate) pmol. The mean values (n = 3) of analytical recovery for L‐ and D‐lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L‐ and D‐lactate in CSF (n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 µmol/L, respectively. Copyright © 2000 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11113927</pmid><doi>10.1002/1099-0801(200011)14:7<474::AID-BMC995>3.0.CO;2-3</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Calibration Chromatography, High Pressure Liquid - methods Humans Lactic Acid - cerebrospinal fluid Ligands Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Stereoisomerism |
| title | Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography |
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