The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH(2)) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus)
Abstract Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. The present study was conducted to determine whether the GHRP, KP-102, specifically stimulates GH release in a teleost. Tilapia (...
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| Veröffentlicht in: | Journal of endocrinology 2000-12, Vol.167 (3), p.R7-10 |
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| creator | Shepherd, BS Eckert, SM Parhar, IS Vijayan, MM Wakabayashi, I Hirano, T Grau, EG Chen, TT |
| description | Abstract Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. The present study was conducted to determine whether the GHRP, KP-102, specifically stimulates GH release in a teleost. Tilapia (Oreochromis mossambicus) were given a single intraperitoneal injection of KP-102 (D-Ala-D-beta;-Nal-Ala-Trp-D-Phe-Lys-NH(2)) or bovine GHRH(1-29)-amide or vehicle and blood was sampled at 1, 6 and 12 h after injection. KP-102 was administered at two doses of 1 ng/g and 10 ng/g body weight, whereas GHRH (positive control) was administered at a single dose of 10 ng/g body weight. Plasma levels of tilapia GH and prolactins (tPRL(177) and tPRL(188)) were determined by radioimmunoassay. As expected, GHRH injection significantly (P |
| doi_str_mv | 10.1677/joe.0.167r007 |
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The present study was conducted to determine whether the GHRP, KP-102, specifically stimulates GH release in a teleost. Tilapia (Oreochromis mossambicus) were given a single intraperitoneal injection of KP-102 (D-Ala-D-beta;-Nal-Ala-Trp-D-Phe-Lys-NH(2)) or bovine GHRH(1-29)-amide or vehicle and blood was sampled at 1, 6 and 12 h after injection. KP-102 was administered at two doses of 1 ng/g and 10 ng/g body weight, whereas GHRH (positive control) was administered at a single dose of 10 ng/g body weight. Plasma levels of tilapia GH and prolactins (tPRL(177) and tPRL(188)) were determined by radioimmunoassay. As expected, GHRH injection significantly (P<0.001) elevated plasma GH levels (ng/ml) in tilapia at 6 h post-injection. KP-102 also significantly elevated GH levels (at the low dose) at 6 (P<0.05) and 12 (P<0.01) hours post-injection. There were no significant effects on plasma PRL(s) levels, although mean levels of both PRLs were elevated at 6 h post-injection. These results show for the first time that GHRPs stimulate GH release in teleosts and suggest that the GHRP receptor and possibly a "Ghrelin-like" ligand are also present in lower vertebrates.</description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/joe.0.167r007</identifier><identifier>PMID: 11115782</identifier><identifier>CODEN: JOENAK</identifier><language>eng</language><publisher>Colchester: BioScientifica</publisher><subject>Analysis of Variance ; Animals ; Biological and medical sciences ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Growth Hormone - blood ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Oligopeptides - pharmacology ; Prolactin - blood ; Receptors, Somatotropin - metabolism ; Sermorelin - pharmacology ; Stimulation, Chemical ; Tilapia - physiology ; Time Factors ; Vertebrates: endocrinology</subject><ispartof>Journal of endocrinology, 2000-12, Vol.167 (3), p.R7-10</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b555t-a5c8bd9ecbe3bbea139f65fff3052d94045cf9cd76e85e2d6b8ce723661401f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=890997$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11115782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shepherd, BS</creatorcontrib><creatorcontrib>Eckert, SM</creatorcontrib><creatorcontrib>Parhar, IS</creatorcontrib><creatorcontrib>Vijayan, MM</creatorcontrib><creatorcontrib>Wakabayashi, I</creatorcontrib><creatorcontrib>Hirano, T</creatorcontrib><creatorcontrib>Grau, EG</creatorcontrib><creatorcontrib>Chen, TT</creatorcontrib><title>The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH(2)) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus)</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description>Abstract Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. The present study was conducted to determine whether the GHRP, KP-102, specifically stimulates GH release in a teleost. Tilapia (Oreochromis mossambicus) were given a single intraperitoneal injection of KP-102 (D-Ala-D-beta;-Nal-Ala-Trp-D-Phe-Lys-NH(2)) or bovine GHRH(1-29)-amide or vehicle and blood was sampled at 1, 6 and 12 h after injection. KP-102 was administered at two doses of 1 ng/g and 10 ng/g body weight, whereas GHRH (positive control) was administered at a single dose of 10 ng/g body weight. Plasma levels of tilapia GH and prolactins (tPRL(177) and tPRL(188)) were determined by radioimmunoassay. As expected, GHRH injection significantly (P<0.001) elevated plasma GH levels (ng/ml) in tilapia at 6 h post-injection. KP-102 also significantly elevated GH levels (at the low dose) at 6 (P<0.05) and 12 (P<0.01) hours post-injection. There were no significant effects on plasma PRL(s) levels, although mean levels of both PRLs were elevated at 6 h post-injection. These results show for the first time that GHRPs stimulate GH release in teleosts and suggest that the GHRP receptor and possibly a "Ghrelin-like" ligand are also present in lower vertebrates.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth Hormone - blood</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Oligopeptides - pharmacology</subject><subject>Prolactin - blood</subject><subject>Receptors, Somatotropin - metabolism</subject><subject>Sermorelin - pharmacology</subject><subject>Stimulation, Chemical</subject><subject>Tilapia - physiology</subject><subject>Time Factors</subject><subject>Vertebrates: endocrinology</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS0EokvhyBVZQkK7Bxc7juP4iFqgiKpFqJwjxxnXrpx1sBOV_ov-5GY3K5BAwpcZjz6_efJD6DWjJ6yS8v1thJN9myiVT9CKlVKRqqbiKVpRWhSESiWO0Iucbyllgkn-HB2x-QhZFyv0cO0AO_ilBxhG3wH--o0wWuD1GdFBkzPSwqjJpQ7765iGeTQ4IOE-k8vzdbHZ4Dz6fgp6hIxvUrwbHXYx9XELOEEAnQH7LdbYeOOC77D12eH1VUwQjUux9xn3MWfdt95MefMSPbM6ZHh1qMfox6eP16fn5OLq85fTDxekFUKMRAtTt50C0wJvW9CMK1sJay2nouhUSUthrDKdrKAWUHRVWxuQBa8qVlJmOT9G7xbdIcWfE-SxmZ0YCEFvIU65kUUpORdqBskCmjTbTGCbIflep_uG0WYXQTNH0OzbXQQz_-YgPLU9dH_ow5_PwNsDoLPRwSa9NT7_5mpFldrJlAvl_I278wma1sdsPGxHb73R_27_vmzny7O_6P97fgQs5rGX</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Shepherd, BS</creator><creator>Eckert, SM</creator><creator>Parhar, IS</creator><creator>Vijayan, MM</creator><creator>Wakabayashi, I</creator><creator>Hirano, T</creator><creator>Grau, EG</creator><creator>Chen, TT</creator><general>BioScientifica</general><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001201</creationdate><title>The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH(2)) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus)</title><author>Shepherd, BS ; Eckert, SM ; Parhar, IS ; Vijayan, MM ; Wakabayashi, I ; Hirano, T ; Grau, EG ; Chen, TT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b555t-a5c8bd9ecbe3bbea139f65fff3052d94045cf9cd76e85e2d6b8ce723661401f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth Hormone - blood</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Oligopeptides - pharmacology</topic><topic>Prolactin - blood</topic><topic>Receptors, Somatotropin - metabolism</topic><topic>Sermorelin - pharmacology</topic><topic>Stimulation, Chemical</topic><topic>Tilapia - physiology</topic><topic>Time Factors</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shepherd, BS</creatorcontrib><creatorcontrib>Eckert, SM</creatorcontrib><creatorcontrib>Parhar, IS</creatorcontrib><creatorcontrib>Vijayan, MM</creatorcontrib><creatorcontrib>Wakabayashi, I</creatorcontrib><creatorcontrib>Hirano, T</creatorcontrib><creatorcontrib>Grau, EG</creatorcontrib><creatorcontrib>Chen, TT</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shepherd, BS</au><au>Eckert, SM</au><au>Parhar, IS</au><au>Vijayan, MM</au><au>Wakabayashi, I</au><au>Hirano, T</au><au>Grau, EG</au><au>Chen, TT</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH(2)) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus)</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>167</volume><issue>3</issue><spage>R7</spage><epage>10</epage><pages>R7-10</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><coden>JOENAK</coden><abstract>Abstract Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. The present study was conducted to determine whether the GHRP, KP-102, specifically stimulates GH release in a teleost. Tilapia (Oreochromis mossambicus) were given a single intraperitoneal injection of KP-102 (D-Ala-D-beta;-Nal-Ala-Trp-D-Phe-Lys-NH(2)) or bovine GHRH(1-29)-amide or vehicle and blood was sampled at 1, 6 and 12 h after injection. KP-102 was administered at two doses of 1 ng/g and 10 ng/g body weight, whereas GHRH (positive control) was administered at a single dose of 10 ng/g body weight. Plasma levels of tilapia GH and prolactins (tPRL(177) and tPRL(188)) were determined by radioimmunoassay. As expected, GHRH injection significantly (P<0.001) elevated plasma GH levels (ng/ml) in tilapia at 6 h post-injection. KP-102 also significantly elevated GH levels (at the low dose) at 6 (P<0.05) and 12 (P<0.01) hours post-injection. There were no significant effects on plasma PRL(s) levels, although mean levels of both PRLs were elevated at 6 h post-injection. These results show for the first time that GHRPs stimulate GH release in teleosts and suggest that the GHRP receptor and possibly a "Ghrelin-like" ligand are also present in lower vertebrates.</abstract><cop>Colchester</cop><pub>BioScientifica</pub><pmid>11115782</pmid><doi>10.1677/joe.0.167r007</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis of Variance Animals Biological and medical sciences Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Growth Hormone - blood Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Oligopeptides - pharmacology Prolactin - blood Receptors, Somatotropin - metabolism Sermorelin - pharmacology Stimulation, Chemical Tilapia - physiology Time Factors Vertebrates: endocrinology |
| title | The hexapeptide KP-102 (D-ala-D-beta-Nal-ala-trp-D-phe-lys-NH(2)) stimulates growth hormone release in a cichlid fish (Ooreochromis mossambicus) |
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