Recovery of Developmentally Defined Gene Sets from High-Density cDNA Macroarrays

New technologies for isolating differentially expressed genes from large arrayed cDNA libraries are reported. These methods can be used to identify genes that lie downstream of developmentally important transcription factors and genes that are expressed in specific tissues, processes, or stages of e...

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Veröffentlicht in:	Developmental biology 2000-12, Vol.228 (2), p.270-286
Hauptverfasser:	Rast, Jonathan P, Amore, Gabriele, Calestani, Cristina, Livi, Carolina B, Ransick, Andrew, Davidson, Eric H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified arrayed cDNA library DNA Probes DNA, Complementary Embryo, Nonmammalian - physiology Gene Expression Regulation, Developmental Gene Library Genes, Reporter Green Fluorescent Proteins Luminescent Proteins - analysis Luminescent Proteins - genetics macroarray Oligonucleotide Array Sequence Analysis RNA, Messenger - genetics sea urchin Sea Urchins - embryology Sea Urchins - genetics Strongylocentrotus purpuratus subtractive hybridization Transcription Factors - metabolism
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