Calcium-Induced Refolding of the Calmodulin V136G Mutant Studied by NMR Spectroscopy:  Evidence for Interaction between the Two Globular Domains

The Ca2+ titration of the 15N-labeled mutant V136G calmodulin has been monitored using 1H−15N HSQC NMR spectra. Up to a [Ca2+]/[CaM] ratio of 2, the Ca2+ ions bind predominantly to sites I and II on the N-domain in contrast with the behavior of the wild-type calmodulin where the C-terminal domain ha...

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