Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene....
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| Veröffentlicht in: | Research in microbiology 1991-06, Vol.142 (5), p.565-572 |
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| creator | Debue, M Gautier, P Hackel, C Van Elsen, A Herzog, A Bigaignon, G Bollen, A |
| description | Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the
Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the
OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the
OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of
B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Des oligonucléotides ont été utilisés pour l'amplification de régions d'ADN spécifiques du plasmide linéaire porté par le spirochète
Borrelia burgdorferi. Une paire d'amorces, OspApc3/pc4, identifie un fragment d'ADN de 442 pb situé dans le gène
OspA; une seconde paire d'amorces, Ospbpc3/pc4, entraîne l'amplification d'un fragment d'ADN de 176 pb localisé dans le gène
OspB. Les amorces basées sur ce dernier gène se révèlent supérieures aux premières pour la discrimination des souches de
B. burgdorfert américaines ou européennes, par rapport aux espèces apparentées. Le système d'amplification détecte jusqu'à 4 spirochètes par réaction et convient pour l'identification de l'agent pathogène dans des fluides biologiques tels que les liquides synoviaux ou cérébrospinaux. |
| doi_str_mv | 10.1016/0923-2508(91)90189-H |
| format | Article |
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Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the
OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the
OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of
B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Des oligonucléotides ont été utilisés pour l'amplification de régions d'ADN spécifiques du plasmide linéaire porté par le spirochète
Borrelia burgdorferi. Une paire d'amorces, OspApc3/pc4, identifie un fragment d'ADN de 442 pb situé dans le gène
OspA; une seconde paire d'amorces, Ospbpc3/pc4, entraîne l'amplification d'un fragment d'ADN de 176 pb localisé dans le gène
OspB. Les amorces basées sur ce dernier gène se révèlent supérieures aux premières pour la discrimination des souches de
B. burgdorfert américaines ou européennes, par rapport aux espèces apparentées. Le système d'amplification détecte jusqu'à 4 spirochètes par réaction et convient pour l'identification de l'agent pathogène dans des fluides biologiques tels que les liquides synoviaux ou cérébrospinaux.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/0923-2508(91)90189-H</identifier><identifier>PMID: 1947428</identifier><language>eng</language><publisher>France: Elsevier SAS</publisher><subject>Borrelia burgdorferi Group - isolation & purification ; Borrelia burgdorferi, Lyme disease, PCR ; Borrelia burgdorferi, Maladie de Lyme, PCR ; borreliosis ; Diagnosis, Carebrospinal and Synovial fluids ; Diagnostic, Liquides synoviaux et céphalorachidiens ; Electrophoresis, Agar Gel ; Humans ; In Vitro Techniques ; Lyme Disease - diagnosis ; Lyme Disease - microbiology ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Synovial Fluid - microbiology</subject><ispartof>Research in microbiology, 1991-06, Vol.142 (5), p.565-572</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-559d7add8d4af813b808c9bf2d54cc7da5fdfc5c390b89d35cdf5616fee920703</citedby><cites>FETCH-LOGICAL-c434t-559d7add8d4af813b808c9bf2d54cc7da5fdfc5c390b89d35cdf5616fee920703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0923-2508(91)90189-H$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1947428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debue, M</creatorcontrib><creatorcontrib>Gautier, P</creatorcontrib><creatorcontrib>Hackel, C</creatorcontrib><creatorcontrib>Van Elsen, A</creatorcontrib><creatorcontrib>Herzog, A</creatorcontrib><creatorcontrib>Bigaignon, G</creatorcontrib><creatorcontrib>Bollen, A</creatorcontrib><title>Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the
Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the
OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the
OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of
B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Des oligonucléotides ont été utilisés pour l'amplification de régions d'ADN spécifiques du plasmide linéaire porté par le spirochète
Borrelia burgdorferi. Une paire d'amorces, OspApc3/pc4, identifie un fragment d'ADN de 442 pb situé dans le gène
OspA; une seconde paire d'amorces, Ospbpc3/pc4, entraîne l'amplification d'un fragment d'ADN de 176 pb localisé dans le gène
OspB. Les amorces basées sur ce dernier gène se révèlent supérieures aux premières pour la discrimination des souches de
B. burgdorfert américaines ou européennes, par rapport aux espèces apparentées. Le système d'amplification détecte jusqu'à 4 spirochètes par réaction et convient pour l'identification de l'agent pathogène dans des fluides biologiques tels que les liquides synoviaux ou cérébrospinaux.</description><subject>Borrelia burgdorferi Group - isolation & purification</subject><subject>Borrelia burgdorferi, Lyme disease, PCR</subject><subject>Borrelia burgdorferi, Maladie de Lyme, PCR</subject><subject>borreliosis</subject><subject>Diagnosis, Carebrospinal and Synovial fluids</subject><subject>Diagnostic, Liquides synoviaux et céphalorachidiens</subject><subject>Electrophoresis, Agar Gel</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Lyme Disease - diagnosis</subject><subject>Lyme Disease - microbiology</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Synovial Fluid - microbiology</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9P3DAUxK0KRBfab1Akn1B7CPhvHF-QgFIWCYlLe7Yc-3lxlcRbO0Hab99sg-gNTu8wv5knzSD0hZJzSmh9QTTjFZOk-arpN01oo6v1B7SiqtaVoowfoNUr8hEdl_KbECqVEkfoiGqhBGtWyH6HEdwY04BTwNcpZ-iixe2UNz7lADniOOA2pi5torMdLrbfdlDwVOKwweMT4G3qdj1kWwC7JzvTGeySaEuxu0_oMNiuwOeXe4J-_bj9ebOuHh7v7m-uHionuBgrKbVX1vvGCxsaytuGNE63gXkpnFPeyuCDk45r0jbac-l8kDWtA4BmRBF-gs6W3G1OfyYoo-ljcdB1doA0FaOYqGtd83dBWlNOJWMzKBbQ5VRKhmC2OfY27wwlZj-B2fdr9v0aTc2_Ccx6tp2-5E9tD_6_ael81i8XHeY2niNkU1yEwYGPeZ7C-BTffvAXZOOXmA</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Debue, M</creator><creator>Gautier, P</creator><creator>Hackel, C</creator><creator>Van Elsen, A</creator><creator>Herzog, A</creator><creator>Bigaignon, G</creator><creator>Bollen, A</creator><general>Elsevier SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910601</creationdate><title>Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay</title><author>Debue, M ; Gautier, P ; Hackel, C ; Van Elsen, A ; Herzog, A ; Bigaignon, G ; Bollen, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-559d7add8d4af813b808c9bf2d54cc7da5fdfc5c390b89d35cdf5616fee920703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Borrelia burgdorferi Group - isolation & purification</topic><topic>Borrelia burgdorferi, Lyme disease, PCR</topic><topic>Borrelia burgdorferi, Maladie de Lyme, PCR</topic><topic>borreliosis</topic><topic>Diagnosis, Carebrospinal and Synovial fluids</topic><topic>Diagnostic, Liquides synoviaux et céphalorachidiens</topic><topic>Electrophoresis, Agar Gel</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Lyme Disease - diagnosis</topic><topic>Lyme Disease - microbiology</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Synovial Fluid - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debue, M</creatorcontrib><creatorcontrib>Gautier, P</creatorcontrib><creatorcontrib>Hackel, C</creatorcontrib><creatorcontrib>Van Elsen, A</creatorcontrib><creatorcontrib>Herzog, A</creatorcontrib><creatorcontrib>Bigaignon, G</creatorcontrib><creatorcontrib>Bollen, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debue, M</au><au>Gautier, P</au><au>Hackel, C</au><au>Van Elsen, A</au><au>Herzog, A</au><au>Bigaignon, G</au><au>Bollen, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>142</volume><issue>5</issue><spage>565</spage><epage>572</epage><pages>565-572</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the
Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the
OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the
OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of
B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Des oligonucléotides ont été utilisés pour l'amplification de régions d'ADN spécifiques du plasmide linéaire porté par le spirochète
Borrelia burgdorferi. Une paire d'amorces, OspApc3/pc4, identifie un fragment d'ADN de 442 pb situé dans le gène
OspA; une seconde paire d'amorces, Ospbpc3/pc4, entraîne l'amplification d'un fragment d'ADN de 176 pb localisé dans le gène
OspB. Les amorces basées sur ce dernier gène se révèlent supérieures aux premières pour la discrimination des souches de
B. burgdorfert américaines ou européennes, par rapport aux espèces apparentées. Le système d'amplification détecte jusqu'à 4 spirochètes par réaction et convient pour l'identification de l'agent pathogène dans des fluides biologiques tels que les liquides synoviaux ou cérébrospinaux.</abstract><cop>France</cop><pub>Elsevier SAS</pub><pmid>1947428</pmid><doi>10.1016/0923-2508(91)90189-H</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-2508 |
| ispartof | Research in microbiology, 1991-06, Vol.142 (5), p.565-572 |
| issn | 0923-2508 1769-7123 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72466963 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Borrelia burgdorferi Group - isolation & purification Borrelia burgdorferi, Lyme disease, PCR Borrelia burgdorferi, Maladie de Lyme, PCR borreliosis Diagnosis, Carebrospinal and Synovial fluids Diagnostic, Liquides synoviaux et céphalorachidiens Electrophoresis, Agar Gel Humans In Vitro Techniques Lyme Disease - diagnosis Lyme Disease - microbiology polymerase chain reaction Polymerase Chain Reaction - methods Synovial Fluid - microbiology |
| title | Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay |
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