Expression of antibody Fab domains on bacteriophage surfaces. Potential use for antibody selection

We describe a method for expressing an antibody Fab fragment on the surface of M13 filamentous bacteriophage. The L chain gene, preceded by an Escherichia coli signal sequence, is linked to the 5' end of the gene for the M13 major coat protein. A partial H chain gene, preceded by an E. coli sig...

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Veröffentlicht in:	The Journal of immunology (1950) 1991-11, Vol.147 (10), p.3610-3614
Hauptverfasser:	Chang, CN, Landolfi, NF, Queen, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chromatography, Affinity Coliphages - genetics Escherichia coli - immunology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Immunoglobulin Fab Fragments - genetics Membrane Proteins - immunology Methods. Procedures. Technologies phage M13 Plasmids Polymerase Chain Reaction Receptors, Interleukin-2 - immunology Recombinant Proteins - immunology Vectors (cloning, transfer, expression). Insertion sequences and transposons
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