Modulation by pentobarbital of neutrophil responses to inhaled E. coli endotoxin in sheep: role of lung epithelium
Neutrophils (PMNs) are implicated in the pathogenesis of acute respiratory distress syndrome (ARDS). The role of the epithelium in the modulation of PMN migration within the lungs was examined. Epithelial integrity and PMN concentrations in the lung air spaces and lymph were measured in sheep anaest...
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description | Neutrophils (PMNs) are implicated in the pathogenesis of acute respiratory distress syndrome (ARDS). The role of the epithelium in the modulation of PMN migration within the lungs was examined. Epithelial integrity and PMN concentrations in the lung air spaces and lymph were measured in sheep anaesthetized with either halothane (1-2.5%) or intravenous pentobarbital (12+/-4 mg x kg(-1) x h(-1)). Ventilation with an aerosol containing 25 mg Escherichia Coli endotoxin (lipopolysaccharide; LPS) effected neutrophil recruitment to the air spaces. Lymphatic clearance of aerosolized 99mTc-DTPA provided an index of epithelial integrity. Three hours after the deposition of LPS, the lung lining fluid of sheep anaesthetized with halothane (n=7) had 4.9+/-3.2x10(6) PMN.mL(-1), but the lung lymph had almost no PMNs (3+/-8%). Sheep anaesthetized with pentobarbital (n=6) had fewer PMNs in the air spaces (2.4+/-1.2X10(6) mL(-1)) and more PMNs in the lung lymph (30+/-20%). Control sheep (n=5) that received no LPS had almost no PMNs in the airspaces or lung lymph, regardless of the anaesthesia. Three additional sheep that remained awake after receiving LPS also had no PMNs in the lung lymph. The PMN fraction in the lung lymph correlated well with the extra-alveolar epithelial permeability measured by lymphatic clearance of aerosolized diethylenetriamine penta-acetic acid (r=0.81, p |
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The role of the epithelium in the modulation of PMN migration within the lungs was examined. Epithelial integrity and PMN concentrations in the lung air spaces and lymph were measured in sheep anaesthetized with either halothane (1-2.5%) or intravenous pentobarbital (12+/-4 mg x kg(-1) x h(-1)). Ventilation with an aerosol containing 25 mg Escherichia Coli endotoxin (lipopolysaccharide; LPS) effected neutrophil recruitment to the air spaces. Lymphatic clearance of aerosolized 99mTc-DTPA provided an index of epithelial integrity. Three hours after the deposition of LPS, the lung lining fluid of sheep anaesthetized with halothane (n=7) had 4.9+/-3.2x10(6) PMN.mL(-1), but the lung lymph had almost no PMNs (3+/-8%). Sheep anaesthetized with pentobarbital (n=6) had fewer PMNs in the air spaces (2.4+/-1.2X10(6) mL(-1)) and more PMNs in the lung lymph (30+/-20%). Control sheep (n=5) that received no LPS had almost no PMNs in the airspaces or lung lymph, regardless of the anaesthesia. Three additional sheep that remained awake after receiving LPS also had no PMNs in the lung lymph. The PMN fraction in the lung lymph correlated well with the extra-alveolar epithelial permeability measured by lymphatic clearance of aerosolized diethylenetriamine penta-acetic acid (r=0.81, p<0.001). Aerosolized lipopolysaccharide recruits neutrophils into the lungs of sheep, but they appear to remain in the airspaces unless extra-alveolar permeability is increased by agents such as pentobarbital.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1034/j.1399-3003.2000.16d22.x</identifier><identifier>PMID: 11106215</identifier><language>eng</language><publisher>Leeds: Eur Respiratory Soc</publisher><subject>Analysis of Variance ; Animals ; Bacterial diseases ; Bacterial diseases of the respiratory system ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid - chemistry ; Endotoxins - administration & dosage ; Epithelium - drug effects ; Escherichia coli ; Human bacterial diseases ; Hypnotics and Sedatives - pharmacology ; Infectious diseases ; Interleukin-8 - analysis ; Lung - cytology ; Lung - drug effects ; Lymph - chemistry ; Medical sciences ; Neutrophils - drug effects ; Neutrophils - metabolism ; Pentobarbital - pharmacology ; Sheep ; Statistics, Nonparametric</subject><ispartof>The European respiratory journal, 2000-10, Vol.16 (4), p.697-703</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-bf87ab8183ff75fd4864b23439146c36cc2b2331cb2979f21cb456661fe73843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1531731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11106215$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peterson, BT</creatorcontrib><creatorcontrib>Miller, EJ</creatorcontrib><creatorcontrib>Griffith, DE</creatorcontrib><creatorcontrib>Rowjee, R</creatorcontrib><creatorcontrib>McWaters, P</creatorcontrib><title>Modulation by pentobarbital of neutrophil responses to inhaled E. coli endotoxin in sheep: role of lung epithelium</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>Neutrophils (PMNs) are implicated in the pathogenesis of acute respiratory distress syndrome (ARDS). The role of the epithelium in the modulation of PMN migration within the lungs was examined. Epithelial integrity and PMN concentrations in the lung air spaces and lymph were measured in sheep anaesthetized with either halothane (1-2.5%) or intravenous pentobarbital (12+/-4 mg x kg(-1) x h(-1)). Ventilation with an aerosol containing 25 mg Escherichia Coli endotoxin (lipopolysaccharide; LPS) effected neutrophil recruitment to the air spaces. Lymphatic clearance of aerosolized 99mTc-DTPA provided an index of epithelial integrity. Three hours after the deposition of LPS, the lung lining fluid of sheep anaesthetized with halothane (n=7) had 4.9+/-3.2x10(6) PMN.mL(-1), but the lung lymph had almost no PMNs (3+/-8%). Sheep anaesthetized with pentobarbital (n=6) had fewer PMNs in the air spaces (2.4+/-1.2X10(6) mL(-1)) and more PMNs in the lung lymph (30+/-20%). Control sheep (n=5) that received no LPS had almost no PMNs in the airspaces or lung lymph, regardless of the anaesthesia. Three additional sheep that remained awake after receiving LPS also had no PMNs in the lung lymph. The PMN fraction in the lung lymph correlated well with the extra-alveolar epithelial permeability measured by lymphatic clearance of aerosolized diethylenetriamine penta-acetic acid (r=0.81, p<0.001). Aerosolized lipopolysaccharide recruits neutrophils into the lungs of sheep, but they appear to remain in the airspaces unless extra-alveolar permeability is increased by agents such as pentobarbital.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the respiratory system</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Endotoxins - administration & dosage</subject><subject>Epithelium - drug effects</subject><subject>Escherichia coli</subject><subject>Human bacterial diseases</subject><subject>Hypnotics and Sedatives - pharmacology</subject><subject>Infectious diseases</subject><subject>Interleukin-8 - analysis</subject><subject>Lung - cytology</subject><subject>Lung - drug effects</subject><subject>Lymph - chemistry</subject><subject>Medical sciences</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - metabolism</subject><subject>Pentobarbital - pharmacology</subject><subject>Sheep</subject><subject>Statistics, Nonparametric</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1v1DAQhi0EokvhLyAfgFuCx3achBuqyodUxKV3y3EmjVeOHexEbP99E3ZFT-PxPPOO9BBCgZXAhPx8LEG0bSEYEyVnbPtVPefl6QU5_B-8JAfWMlFAK9QVeZPzkTFQUsBrcgUATHGoDiT9iv3qzeJioN0jnTEssTOpc4vxNA404LqkOI_O04R5jiFjpkukLozGY09vS2qjdxRDH5d4cmGb0Dwizl9oih73DL-GB4qzW0b0bp3ekleD8RnfXeo1uf92e3_zo7j7_f3nzde7woqaLUU3NLXpGmjEMNTV0MtGyY4LKVqQygplLd9aAbbjbd0OfHvISikFA9aikeKafDrHzin-WTEvenLZovcmYFyzrrmsJHC-gc0ZtCnmnHDQc3KTSY8amN5166Pererdqt5163-69WlbfX-5sXYT9s-LF78b8OECmGyNH5IJ1uVnrhJQC9iwj2dsdA_jX5dQ58l4v6WCxnQEpaVWbS2eAK_Alr8</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Peterson, BT</creator><creator>Miller, EJ</creator><creator>Griffith, DE</creator><creator>Rowjee, R</creator><creator>McWaters, P</creator><general>Eur Respiratory Soc</general><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Modulation by pentobarbital of neutrophil responses to inhaled E. coli endotoxin in sheep: role of lung epithelium</title><author>Peterson, BT ; Miller, EJ ; Griffith, DE ; Rowjee, R ; McWaters, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-bf87ab8183ff75fd4864b23439146c36cc2b2331cb2979f21cb456661fe73843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the respiratory system</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Endotoxins - administration & dosage</topic><topic>Epithelium - drug effects</topic><topic>Escherichia coli</topic><topic>Human bacterial diseases</topic><topic>Hypnotics and Sedatives - pharmacology</topic><topic>Infectious diseases</topic><topic>Interleukin-8 - analysis</topic><topic>Lung - cytology</topic><topic>Lung - drug effects</topic><topic>Lymph - chemistry</topic><topic>Medical sciences</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - metabolism</topic><topic>Pentobarbital - pharmacology</topic><topic>Sheep</topic><topic>Statistics, Nonparametric</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peterson, BT</creatorcontrib><creatorcontrib>Miller, EJ</creatorcontrib><creatorcontrib>Griffith, DE</creatorcontrib><creatorcontrib>Rowjee, R</creatorcontrib><creatorcontrib>McWaters, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peterson, BT</au><au>Miller, EJ</au><au>Griffith, DE</au><au>Rowjee, R</au><au>McWaters, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation by pentobarbital of neutrophil responses to inhaled E. coli endotoxin in sheep: role of lung epithelium</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>16</volume><issue>4</issue><spage>697</spage><epage>703</epage><pages>697-703</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>Neutrophils (PMNs) are implicated in the pathogenesis of acute respiratory distress syndrome (ARDS). The role of the epithelium in the modulation of PMN migration within the lungs was examined. Epithelial integrity and PMN concentrations in the lung air spaces and lymph were measured in sheep anaesthetized with either halothane (1-2.5%) or intravenous pentobarbital (12+/-4 mg x kg(-1) x h(-1)). Ventilation with an aerosol containing 25 mg Escherichia Coli endotoxin (lipopolysaccharide; LPS) effected neutrophil recruitment to the air spaces. Lymphatic clearance of aerosolized 99mTc-DTPA provided an index of epithelial integrity. Three hours after the deposition of LPS, the lung lining fluid of sheep anaesthetized with halothane (n=7) had 4.9+/-3.2x10(6) PMN.mL(-1), but the lung lymph had almost no PMNs (3+/-8%). Sheep anaesthetized with pentobarbital (n=6) had fewer PMNs in the air spaces (2.4+/-1.2X10(6) mL(-1)) and more PMNs in the lung lymph (30+/-20%). Control sheep (n=5) that received no LPS had almost no PMNs in the airspaces or lung lymph, regardless of the anaesthesia. Three additional sheep that remained awake after receiving LPS also had no PMNs in the lung lymph. The PMN fraction in the lung lymph correlated well with the extra-alveolar epithelial permeability measured by lymphatic clearance of aerosolized diethylenetriamine penta-acetic acid (r=0.81, p<0.001). Aerosolized lipopolysaccharide recruits neutrophils into the lungs of sheep, but they appear to remain in the airspaces unless extra-alveolar permeability is increased by agents such as pentobarbital.</abstract><cop>Leeds</cop><pub>Eur Respiratory Soc</pub><pmid>11106215</pmid><doi>10.1034/j.1399-3003.2000.16d22.x</doi><tpages>7</tpages></addata></record> |
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subjects | Analysis of Variance Animals Bacterial diseases Bacterial diseases of the respiratory system Biological and medical sciences Bronchoalveolar Lavage Fluid - chemistry Endotoxins - administration & dosage Epithelium - drug effects Escherichia coli Human bacterial diseases Hypnotics and Sedatives - pharmacology Infectious diseases Interleukin-8 - analysis Lung - cytology Lung - drug effects Lymph - chemistry Medical sciences Neutrophils - drug effects Neutrophils - metabolism Pentobarbital - pharmacology Sheep Statistics, Nonparametric |
title | Modulation by pentobarbital of neutrophil responses to inhaled E. coli endotoxin in sheep: role of lung epithelium |
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