A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors
The expression of the nicotinic acetylcholine receptor (AChR) in vertebrate striated muscle is regulated both during development and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the mouse alpha-subunit AChR gene, we have isolate...
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| Veröffentlicht in: | The Journal of biological chemistry 1991-11, Vol.266 (33), p.22588-22596 |
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| creator | PRODY, C. A MERLIE, J. P |
| description | The expression of the nicotinic acetylcholine receptor (AChR) in vertebrate striated muscle is regulated both during development
and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the
mouse alpha-subunit AChR gene, we have isolated and analyzed the alpha-gene 5'-flanking region. Primer extension and RNase
protection analysis showed that transcription initiates at 2 major and 12 minor sites close to the translational initiation
site. Using a series of plasmids in which segments of the 5'-flanking region were linked to the bacterial chloramphenicol
acetyltransferase (CAT) gene, we have defined an 86-base pair enhancer sequence that is active in C2 myotubes but not in C2
myoblasts or NIH3T3 fibroblasts. This enhancer contains three putative binding sites for myoD1, and the 5'-upstream regions
linked to CAT were transactivated by the muscle regulatory factors, myoD1, and myogenin. Transactivation by MRF4 differed
with the specific alpha-subunit construct tested. Whereas the alpha-subunit CAT constructs containing both the homologous
as well as the heterologous myosin light chain 1 promoter were transactivated by myoD1 and myogenin, only the constructs containing
their homologous promoter were transactivated by MRF4. Thus, an 86-base pair sequence of the alpha-subunit gene contains the
information necessary for developmental specificity and responsiveness to myogenic factors. |
| doi_str_mv | 10.1016/S0021-9258(18)54612-3 |
| format | Article |
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and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the
mouse alpha-subunit AChR gene, we have isolated and analyzed the alpha-gene 5'-flanking region. Primer extension and RNase
protection analysis showed that transcription initiates at 2 major and 12 minor sites close to the translational initiation
site. Using a series of plasmids in which segments of the 5'-flanking region were linked to the bacterial chloramphenicol
acetyltransferase (CAT) gene, we have defined an 86-base pair enhancer sequence that is active in C2 myotubes but not in C2
myoblasts or NIH3T3 fibroblasts. This enhancer contains three putative binding sites for myoD1, and the 5'-upstream regions
linked to CAT were transactivated by the muscle regulatory factors, myoD1, and myogenin. Transactivation by MRF4 differed
with the specific alpha-subunit construct tested. Whereas the alpha-subunit CAT constructs containing both the homologous
as well as the heterologous myosin light chain 1 promoter were transactivated by myoD1 and myogenin, only the constructs containing
their homologous promoter were transactivated by MRF4. Thus, an 86-base pair sequence of the alpha-subunit gene contains the
information necessary for developmental specificity and responsiveness to myogenic factors.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)54612-3</identifier><identifier>PMID: 1658001</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>3T3 Cells ; Animals ; Base Sequence ; Biological and medical sciences ; Cell Line ; Chickens ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Macromolecular Substances ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Muscle Proteins - physiology ; Muscles - physiology ; MyoD Protein ; Myogenin ; Nuclear Proteins - physiology ; Phosphoproteins - physiology ; Receptors, Cholinergic - genetics ; Recombinant Fusion Proteins - metabolism ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Space life sciences ; Trans-Activators - physiology ; Transcription. Transcription factor. Splicing. Rna processing ; Transcriptional Activation ; Transfection</subject><ispartof>The Journal of biological chemistry, 1991-11, Vol.266 (33), p.22588-22596</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-5a8bd9b99cc8b514e35de06c757e82688c2410f97acf399064deae2502a9da503</citedby><cites>FETCH-LOGICAL-c409t-5a8bd9b99cc8b514e35de06c757e82688c2410f97acf399064deae2502a9da503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5093499$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1658001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PRODY, C. A</creatorcontrib><creatorcontrib>MERLIE, J. P</creatorcontrib><title>A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The expression of the nicotinic acetylcholine receptor (AChR) in vertebrate striated muscle is regulated both during development
and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the
mouse alpha-subunit AChR gene, we have isolated and analyzed the alpha-gene 5'-flanking region. Primer extension and RNase
protection analysis showed that transcription initiates at 2 major and 12 minor sites close to the translational initiation
site. Using a series of plasmids in which segments of the 5'-flanking region were linked to the bacterial chloramphenicol
acetyltransferase (CAT) gene, we have defined an 86-base pair enhancer sequence that is active in C2 myotubes but not in C2
myoblasts or NIH3T3 fibroblasts. This enhancer contains three putative binding sites for myoD1, and the 5'-upstream regions
linked to CAT were transactivated by the muscle regulatory factors, myoD1, and myogenin. Transactivation by MRF4 differed
with the specific alpha-subunit construct tested. Whereas the alpha-subunit CAT constructs containing both the homologous
as well as the heterologous myosin light chain 1 promoter were transactivated by myoD1 and myogenin, only the constructs containing
their homologous promoter were transactivated by MRF4. Thus, an 86-base pair sequence of the alpha-subunit gene contains the
information necessary for developmental specificity and responsiveness to myogenic factors.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chickens</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Macromolecular Substances</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Muscle Proteins - physiology</subject><subject>Muscles - physiology</subject><subject>MyoD Protein</subject><subject>Myogenin</subject><subject>Nuclear Proteins - physiology</subject><subject>Phosphoproteins - physiology</subject><subject>Receptors, Cholinergic - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Space life sciences</subject><subject>Trans-Activators - physiology</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkctu3SAURVHVKL1J-wmRGFRVM3AD2NgwjKK-pEgdNJU6Q_j4-JoWPwq41f2M_HG5DyVMkNhrs2EfQq44-8AZr2--MyZ4oYVU77m6llXNRVG-IBvOVFmUkv98STZPyCtyEeMvllel-Tk557VUjPENebylHf5FPy8jTsl6aqeOJhfjikVcEFzvgOI02AkwUDfRNCAd5zUijb_R494yrhE8UguYdh6G2bsJaUDAJc2BWr8Mtohru04u0S0etO3qbcKOtjs67uZ8mFN6C5mPr8lZb33EN6f9kvz49PHh7ktx_-3z17vb-wIqplMhrWo73WoNoFrJKyxlh6yGRjaoRK0UiIqzXjcW-lJrVlcdWhSSCas7K1l5Sd4d713C_GfFmMzoIqD3dsL8P9OIqtJaNhmURxDCHGPA3izBjTbsDGdmPwpzGIXZ92y4ModRmDL7rk4Bazti9-w6dp_1tyfdRrC-D7ljF58wyXSZH_CMDW47_HMBTetmGHA0oq5zjBE5V5X_AWsDoIg</recordid><startdate>19911125</startdate><enddate>19911125</enddate><creator>PRODY, C. A</creator><creator>MERLIE, J. P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911125</creationdate><title>A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors</title><author>PRODY, C. A ; MERLIE, J. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-5a8bd9b99cc8b514e35de06c757e82688c2410f97acf399064deae2502a9da503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chickens</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>Enhancer Elements, Genetic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Macromolecular Substances</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Muscle Proteins - physiology</topic><topic>Muscles - physiology</topic><topic>MyoD Protein</topic><topic>Myogenin</topic><topic>Nuclear Proteins - physiology</topic><topic>Phosphoproteins - physiology</topic><topic>Receptors, Cholinergic - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Space life sciences</topic><topic>Trans-Activators - physiology</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transcriptional Activation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PRODY, C. A</creatorcontrib><creatorcontrib>MERLIE, J. P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PRODY, C. A</au><au>MERLIE, J. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-11-25</date><risdate>1991</risdate><volume>266</volume><issue>33</issue><spage>22588</spage><epage>22596</epage><pages>22588-22596</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The expression of the nicotinic acetylcholine receptor (AChR) in vertebrate striated muscle is regulated both during development
and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the
mouse alpha-subunit AChR gene, we have isolated and analyzed the alpha-gene 5'-flanking region. Primer extension and RNase
protection analysis showed that transcription initiates at 2 major and 12 minor sites close to the translational initiation
site. Using a series of plasmids in which segments of the 5'-flanking region were linked to the bacterial chloramphenicol
acetyltransferase (CAT) gene, we have defined an 86-base pair enhancer sequence that is active in C2 myotubes but not in C2
myoblasts or NIH3T3 fibroblasts. This enhancer contains three putative binding sites for myoD1, and the 5'-upstream regions
linked to CAT were transactivated by the muscle regulatory factors, myoD1, and myogenin. Transactivation by MRF4 differed
with the specific alpha-subunit construct tested. Whereas the alpha-subunit CAT constructs containing both the homologous
as well as the heterologous myosin light chain 1 promoter were transactivated by myoD1 and myogenin, only the constructs containing
their homologous promoter were transactivated by MRF4. Thus, an 86-base pair sequence of the alpha-subunit gene contains the
information necessary for developmental specificity and responsiveness to myogenic factors.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1658001</pmid><doi>10.1016/S0021-9258(18)54612-3</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1991-11, Vol.266 (33), p.22588-22596 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72449957 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3T3 Cells Animals Base Sequence Biological and medical sciences Cell Line Chickens Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene Expression Regulation Macromolecular Substances Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Muscle Proteins - physiology Muscles - physiology MyoD Protein Myogenin Nuclear Proteins - physiology Phosphoproteins - physiology Receptors, Cholinergic - genetics Recombinant Fusion Proteins - metabolism Restriction Mapping Sequence Homology, Nucleic Acid Space life sciences Trans-Activators - physiology Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation Transfection |
| title | A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors |
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