Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry

. The ability of glucocorticoids to induce apoptosis or programmed cell death in mouse thymocytes is well‐established. Measurement of apoptosis‐associated internucleosomal DNA fragmentation through determination of the percentage of fragmented DNA by electrophoresis or centrifugation of whole cell l...

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Veröffentlicht in:	Cell proliferation 1991-09, Vol.24 (5), p.447-459
Hauptverfasser:	Telford, W. G., King, L. E., Fraker, P. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Death Corticosterone - pharmacology Densitometry Dexamethasone - pharmacology DNA - metabolism Dna, deoxyribonucleoproteins Electrophoresis, Agar Gel Endonucleases - antagonists & inhibitors Flow Cytometry Fundamental and applied biological sciences. Psychology Mice Nucleic acids Propidium Protein Biosynthesis Receptors, Glucocorticoid - antagonists & inhibitors Thymus Gland - cytology Thymus Gland - drug effects Thymus Gland - metabolism Transcription, Genetic Zinc - pharmacology
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