Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children
Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children. We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy f...
Gespeichert in:
| Veröffentlicht in: | The American journal of clinical nutrition 2000-12, Vol.72 (6), p.1469-1473 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1473 |
|---|---|
| container_issue | 6 |
| container_start_page | 1469 |
| container_title | The American journal of clinical nutrition |
| container_volume | 72 |
| creator | DELVIN, Edgard E ROZEN, Rima MEROUANI, Aicha GENEST, Jacques JR LAMBERT, Marie |
| description | Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children.
We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy fasting children and related these to MTHFR genotype.
After the subjects fasted, blood samples were collected into EDTA-containing tubes. Plasma, red blood cells, and the buffy coat were immediately stored at -80 degrees C for biochemical and molecular analyses. Plasma total homocysteine was determined by HPLC. Folate and vitamin B-12 were measured by a double-labeled radioimmunoassay, and the genotypic analysis was performed by polymerase chain reaction amplification of genomic DNA extracted from blood leukocytes.
Plasma homocysteine concentrations correlated negatively with folate and vitamin B-12(,) but positively with age (P: < 0. 0001). Whereas folate and vitamin B-12 accounted for 27% and 19% of the variation in homocysteine, respectively, age accounted for 48% of the variation. When the cohort was divided into older (>10 y) and younger (10 y.
Our data show that in a healthy pediatric population, MTHFR genotype played a significant role in determining homocysteine concentrations in older (>10 y), nutritionally stressed children. |
| doi_str_mv | 10.1093/ajcn/72.6.1469 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72440532</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>65269638</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-8f9e6198ceeaf0eddac70793c1b8de16841f380490c3c2a9a67ced3965d885cc3</originalsourceid><addsrcrecordid>eNpdkU1r3DAQhkVpaLZprz0W0UJP9UaytLJ0TEM_AoFckrOYSKOsF1vaSnLB1_7yeolpoZcZGJ73ZeAh5B1nW86MuISDi5ddu1VbLpV5QTbcCN2IlnUvyYYx1jaGq905eV3KgTHeSq1ekXPOOeOyExvy-yaGYcLokKZAR6z7ecCIFWuG_exzCmmAijSjn1yFgvQJY6rzET9TeFrGr77C2Ef6peHtcoqerolSoU6FpkiPA5QR6D6Nyc2lYh-RLgm37wefMb4hZwGGgm_XfUEevn29v_7R3N59v7m-um2c0Ko2OhhU3GiHCIGh9-A61hnh-KP2yJWWPAjNpGFOuBYMqM6hF0btvNY758QF-fTce8zp54Sl2rEvDocBIqap2K6Vku1Eu4Af_gMPacpx-c22ghuplDxB22fI5VRKxmCPuR8hz5Yze1JjT2qWUqvsSc0SeL-2To8j-n_46mIBPq4AFAdDyBBdX_5yWnRLi_gD-R6YxA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>231946642</pqid></control><display><type>article</type><title>Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>DELVIN, Edgard E ; ROZEN, Rima ; MEROUANI, Aicha ; GENEST, Jacques JR ; LAMBERT, Marie</creator><creatorcontrib>DELVIN, Edgard E ; ROZEN, Rima ; MEROUANI, Aicha ; GENEST, Jacques JR ; LAMBERT, Marie</creatorcontrib><description>Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children.
We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy fasting children and related these to MTHFR genotype.
After the subjects fasted, blood samples were collected into EDTA-containing tubes. Plasma, red blood cells, and the buffy coat were immediately stored at -80 degrees C for biochemical and molecular analyses. Plasma total homocysteine was determined by HPLC. Folate and vitamin B-12 were measured by a double-labeled radioimmunoassay, and the genotypic analysis was performed by polymerase chain reaction amplification of genomic DNA extracted from blood leukocytes.
Plasma homocysteine concentrations correlated negatively with folate and vitamin B-12(,) but positively with age (P: < 0. 0001). Whereas folate and vitamin B-12 accounted for 27% and 19% of the variation in homocysteine, respectively, age accounted for 48% of the variation. When the cohort was divided into older (>10 y) and younger (</=10 y) individuals, folate was significantly lower in the older individuals who were homozygous for the mutation (T/T) than in those who were homozygous for the wild-type allele (C/C). Homocysteine was higher in the T/T group than in both the C/C and C/T subgroups aged >10 y.
Our data show that in a healthy pediatric population, MTHFR genotype played a significant role in determining homocysteine concentrations in older (>10 y), nutritionally stressed children.</description><identifier>ISSN: 0002-9165</identifier><identifier>EISSN: 1938-3207</identifier><identifier>DOI: 10.1093/ajcn/72.6.1469</identifier><identifier>PMID: 11101473</identifier><identifier>CODEN: AJCNAC</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Clinical Nutrition</publisher><subject>Adolescent ; Aging - genetics ; Biological and medical sciences ; Chi-Square Distribution ; Child ; Child, Preschool ; Children & youth ; Chromatography, High Pressure Liquid ; Diet ; Fasting - metabolism ; Feeding. Feeding behavior ; Female ; Folic Acid - blood ; Fundamental and applied biological sciences. Psychology ; Genotype ; Homocysteine - blood ; Humans ; Male ; Methylenetetrahydrofolate Reductase (NADPH2) ; Nutrition ; Oxidoreductases Acting on CH-NH Group Donors - genetics ; Plasma ; Polymerase Chain Reaction ; Radioimmunoassay ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vitamin B ; Vitamin B 12 - blood</subject><ispartof>The American journal of clinical nutrition, 2000-12, Vol.72 (6), p.1469-1473</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright American Society for Clinical Nutrition, Inc. Dec 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-8f9e6198ceeaf0eddac70793c1b8de16841f380490c3c2a9a67ced3965d885cc3</citedby><cites>FETCH-LOGICAL-c386t-8f9e6198ceeaf0eddac70793c1b8de16841f380490c3c2a9a67ced3965d885cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=837693$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11101473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DELVIN, Edgard E</creatorcontrib><creatorcontrib>ROZEN, Rima</creatorcontrib><creatorcontrib>MEROUANI, Aicha</creatorcontrib><creatorcontrib>GENEST, Jacques JR</creatorcontrib><creatorcontrib>LAMBERT, Marie</creatorcontrib><title>Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children</title><title>The American journal of clinical nutrition</title><addtitle>Am J Clin Nutr</addtitle><description>Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children.
We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy fasting children and related these to MTHFR genotype.
After the subjects fasted, blood samples were collected into EDTA-containing tubes. Plasma, red blood cells, and the buffy coat were immediately stored at -80 degrees C for biochemical and molecular analyses. Plasma total homocysteine was determined by HPLC. Folate and vitamin B-12 were measured by a double-labeled radioimmunoassay, and the genotypic analysis was performed by polymerase chain reaction amplification of genomic DNA extracted from blood leukocytes.
Plasma homocysteine concentrations correlated negatively with folate and vitamin B-12(,) but positively with age (P: < 0. 0001). Whereas folate and vitamin B-12 accounted for 27% and 19% of the variation in homocysteine, respectively, age accounted for 48% of the variation. When the cohort was divided into older (>10 y) and younger (</=10 y) individuals, folate was significantly lower in the older individuals who were homozygous for the mutation (T/T) than in those who were homozygous for the wild-type allele (C/C). Homocysteine was higher in the T/T group than in both the C/C and C/T subgroups aged >10 y.
Our data show that in a healthy pediatric population, MTHFR genotype played a significant role in determining homocysteine concentrations in older (>10 y), nutritionally stressed children.</description><subject>Adolescent</subject><subject>Aging - genetics</subject><subject>Biological and medical sciences</subject><subject>Chi-Square Distribution</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children & youth</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diet</subject><subject>Fasting - metabolism</subject><subject>Feeding. Feeding behavior</subject><subject>Female</subject><subject>Folic Acid - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Homocysteine - blood</subject><subject>Humans</subject><subject>Male</subject><subject>Methylenetetrahydrofolate Reductase (NADPH2)</subject><subject>Nutrition</subject><subject>Oxidoreductases Acting on CH-NH Group Donors - genetics</subject><subject>Plasma</subject><subject>Polymerase Chain Reaction</subject><subject>Radioimmunoassay</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vitamin B</subject><subject>Vitamin B 12 - blood</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1r3DAQhkVpaLZprz0W0UJP9UaytLJ0TEM_AoFckrOYSKOsF1vaSnLB1_7yeolpoZcZGJ73ZeAh5B1nW86MuISDi5ddu1VbLpV5QTbcCN2IlnUvyYYx1jaGq905eV3KgTHeSq1ekXPOOeOyExvy-yaGYcLokKZAR6z7ecCIFWuG_exzCmmAijSjn1yFgvQJY6rzET9TeFrGr77C2Ef6peHtcoqerolSoU6FpkiPA5QR6D6Nyc2lYh-RLgm37wefMb4hZwGGgm_XfUEevn29v_7R3N59v7m-um2c0Ko2OhhU3GiHCIGh9-A61hnh-KP2yJWWPAjNpGFOuBYMqM6hF0btvNY758QF-fTce8zp54Sl2rEvDocBIqap2K6Vku1Eu4Af_gMPacpx-c22ghuplDxB22fI5VRKxmCPuR8hz5Yze1JjT2qWUqvsSc0SeL-2To8j-n_46mIBPq4AFAdDyBBdX_5yWnRLi_gD-R6YxA</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>DELVIN, Edgard E</creator><creator>ROZEN, Rima</creator><creator>MEROUANI, Aicha</creator><creator>GENEST, Jacques JR</creator><creator>LAMBERT, Marie</creator><general>American Society for Clinical Nutrition</general><general>American Society for Clinical Nutrition, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T7</scope><scope>7TS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20001201</creationdate><title>Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children</title><author>DELVIN, Edgard E ; ROZEN, Rima ; MEROUANI, Aicha ; GENEST, Jacques JR ; LAMBERT, Marie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-8f9e6198ceeaf0eddac70793c1b8de16841f380490c3c2a9a67ced3965d885cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adolescent</topic><topic>Aging - genetics</topic><topic>Biological and medical sciences</topic><topic>Chi-Square Distribution</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children & youth</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Diet</topic><topic>Fasting - metabolism</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Folic Acid - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Homocysteine - blood</topic><topic>Humans</topic><topic>Male</topic><topic>Methylenetetrahydrofolate Reductase (NADPH2)</topic><topic>Nutrition</topic><topic>Oxidoreductases Acting on CH-NH Group Donors - genetics</topic><topic>Plasma</topic><topic>Polymerase Chain Reaction</topic><topic>Radioimmunoassay</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vitamin B</topic><topic>Vitamin B 12 - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DELVIN, Edgard E</creatorcontrib><creatorcontrib>ROZEN, Rima</creatorcontrib><creatorcontrib>MEROUANI, Aicha</creatorcontrib><creatorcontrib>GENEST, Jacques JR</creatorcontrib><creatorcontrib>LAMBERT, Marie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Physical Education Index</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DELVIN, Edgard E</au><au>ROZEN, Rima</au><au>MEROUANI, Aicha</au><au>GENEST, Jacques JR</au><au>LAMBERT, Marie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>72</volume><issue>6</issue><spage>1469</spage><epage>1473</epage><pages>1469-1473</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children.
We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy fasting children and related these to MTHFR genotype.
After the subjects fasted, blood samples were collected into EDTA-containing tubes. Plasma, red blood cells, and the buffy coat were immediately stored at -80 degrees C for biochemical and molecular analyses. Plasma total homocysteine was determined by HPLC. Folate and vitamin B-12 were measured by a double-labeled radioimmunoassay, and the genotypic analysis was performed by polymerase chain reaction amplification of genomic DNA extracted from blood leukocytes.
Plasma homocysteine concentrations correlated negatively with folate and vitamin B-12(,) but positively with age (P: < 0. 0001). Whereas folate and vitamin B-12 accounted for 27% and 19% of the variation in homocysteine, respectively, age accounted for 48% of the variation. When the cohort was divided into older (>10 y) and younger (</=10 y) individuals, folate was significantly lower in the older individuals who were homozygous for the mutation (T/T) than in those who were homozygous for the wild-type allele (C/C). Homocysteine was higher in the T/T group than in both the C/C and C/T subgroups aged >10 y.
Our data show that in a healthy pediatric population, MTHFR genotype played a significant role in determining homocysteine concentrations in older (>10 y), nutritionally stressed children.</abstract><cop>Bethesda, MD</cop><pub>American Society for Clinical Nutrition</pub><pmid>11101473</pmid><doi>10.1093/ajcn/72.6.1469</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9165 |
| ispartof | The American journal of clinical nutrition, 2000-12, Vol.72 (6), p.1469-1473 |
| issn | 0002-9165 1938-3207 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72440532 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adolescent Aging - genetics Biological and medical sciences Chi-Square Distribution Child Child, Preschool Children & youth Chromatography, High Pressure Liquid Diet Fasting - metabolism Feeding. Feeding behavior Female Folic Acid - blood Fundamental and applied biological sciences. Psychology Genotype Homocysteine - blood Humans Male Methylenetetrahydrofolate Reductase (NADPH2) Nutrition Oxidoreductases Acting on CH-NH Group Donors - genetics Plasma Polymerase Chain Reaction Radioimmunoassay Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin B Vitamin B 12 - blood |
| title | Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T19%3A31%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20methylenetetrahydrofolate%20reductase%20genotype,%20age,%20vitamin%20B-12,%20and%20folate%20status%20on%20plasma%20homocysteine%20in%20children&rft.jtitle=The%20American%20journal%20of%20clinical%20nutrition&rft.au=DELVIN,%20Edgard%20E&rft.date=2000-12-01&rft.volume=72&rft.issue=6&rft.spage=1469&rft.epage=1473&rft.pages=1469-1473&rft.issn=0002-9165&rft.eissn=1938-3207&rft.coden=AJCNAC&rft_id=info:doi/10.1093/ajcn/72.6.1469&rft_dat=%3Cproquest_cross%3E65269638%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=231946642&rft_id=info:pmid/11101473&rfr_iscdi=true |